{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T01:44:47Z","timestamp":1790905487361,"version":"4.1.0"},"reference-count":46,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2007,12,1]],"date-time":"2007-12-01T00:00:00Z","timestamp":1196467200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2007,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Human herpesvirus 6 (HHV-6) was detected in specimens from patients hospitalized with symptoms of encephalitis or meningitis. A real-time PCR assay was developed which has a linear dynamic range of 5 to 5 \u00d7 10\n            <jats:sup>6<\/jats:sup>\n            copies of HHV-6 and a sensitivity of five gene copies per reaction. While the assay detects both subtypes, HHV-6A and HHV-6B, it is specific and does not cross-react with a selected specificity panel. A total of 1,482 patient specimens, which were collected between 2003 and 2007, were tested; 26 specimens from 24 patients were found to be positive for HHV-6 by real-time PCR. The HHV-6 detection rate in this population was therefore 1.75%. The majority of the specimens tested (&gt;95%) were cerebrospinal fluid (CSF) specimens. We were able to type 20 of the 26 positive specimens by conventional PCR and sequence analysis; all were HHV-6B. Forty-two percent of the patients were 3 years of age or younger, which may indicate a primary infection in these patients. Given the ages of the remaining patients (from 4 to 81 years), their infections were most probably due to virus reactivations. Where information was available, symptoms of patients included fever (71%), altered mental status (67%), and abnormal CSF profile (75%). Fifty percent of patients of 3 years of age or younger suffered from seizures. The detection of HHV-6 in specimens from patients diagnosed with encephalitis or meningitis, in the absence of a positive PCR result for other agents, strongly suggests a role for HHV-6 in the pathogenesis of these central nervous system diseases.\n          <\/jats:p>","DOI":"10.1128\/jcm.01692-07","type":"journal-article","created":{"date-parts":[[2007,10,18]],"date-time":"2007-10-18T01:25:01Z","timestamp":1192670701000},"page":"3972-3978","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":50,"title":["Detection and Typing of Human Herpesvirus 6 by Molecular Methods in Specimens from Patients Diagnosed with Encephalitis or Meningitis"],"prefix":"10.1128","volume":"45","author":[{"given":"Norma P.","family":"Tavakoli","sequence":"first","affiliation":[{"name":"Wadsworth Center, New York State Department of Health, Albany, New York"},{"name":"School of Public Health, SUNY Albany, Albany, New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seela","family":"Nattanmai","sequence":"additional","affiliation":[{"name":"Wadsworth Center, New York State Department of Health, Albany, New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rene","family":"Hull","sequence":"additional","affiliation":[{"name":"Wadsworth Center, New York State Department of Health, Albany, New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heather","family":"Fusco","sequence":"additional","affiliation":[{"name":"Wadsworth Center, New York State Department of Health, Albany, New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lela","family":"Dzigua","sequence":"additional","affiliation":[{"name":"Wadsworth Center, New York State Department of Health, Albany, New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heng","family":"Wang","sequence":"additional","affiliation":[{"name":"Wadsworth Center, New York State Department of Health, Albany, New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michelle","family":"Dupuis","sequence":"additional","affiliation":[{"name":"Wadsworth Center, New York State Department of Health, Albany, New York"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1542\/peds.105.2.431"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bmt.1703266"},{"key":"e_1_3_2_4_2","first-page":"271","volume":"5","year":"1991","unstructured":"Bertram, G., N. 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