{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,7]],"date-time":"2026-01-07T07:28:35Z","timestamp":1767770915200},"reference-count":25,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2013,12,1]],"date-time":"2013-12-01T00:00:00Z","timestamp":1385856000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2013,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            In our jurisdiction, the Aptima Combo 2 assay (Gen-Probe, Inc.) is used to detect\n            <jats:named-content content-type=\"genus-species\">Neisseria gonorrhoeae<\/jats:named-content>\n            from specimens collected at clinics for sexually transmitted infections (STI) and from select community patients. In addition, swabs are also collected for\n            <jats:named-content content-type=\"genus-species\">N. gonorrhoeae<\/jats:named-content>\n            culture, susceptibility testing, and sequence typing (ST). Since only a small proportion of samples from provincial cases undergo culture, the available trends in antimicrobial susceptibility and predominant strain types may not be representative of all\n            <jats:named-content content-type=\"genus-species\">N. gonorrhoeae<\/jats:named-content>\n            infections. Due to the limitations facing the use of\n            <jats:named-content content-type=\"genus-species\">N. gonorrhoeae<\/jats:named-content>\n            culture to understand these trends in the general community, we performed a molecular analysis for markers of cephalosporin resistance and ST determination by using nucleic acid extracts of specimens sent for Aptima testing. Thirty-four samples submitted for both Aptima testing and\n            <jats:named-content content-type=\"genus-species\">N. gonorrhoeae<\/jats:named-content>\n            culture from the same anatomic location (within 24 h) were included in the study. Sequence type was determined based on the sequence of the\n            <jats:italic>por<\/jats:italic>\n            and\n            <jats:italic>tbpB<\/jats:italic>\n            genes, and amino acid changes in the PBP 2 protein, encoded by the\n            <jats:italic>penA<\/jats:italic>\n            gene, were considered representative for the assessment of antimicrobial susceptibility. Sequence identity of 100% was observed between the sequences obtained from Aptima-analyzed samples and culture samples. Sequencing results showed an association between decreased susceptibility to extended-spectrum cephalosporins (ESC\n            <jats:sup>ds<\/jats:sup>\n            ),\n            <jats:italic>tbp<\/jats:italic>\n            allele 110, ST 1407, and amino acid changes (G545S, I312M, and V316T) in the PBP 2 protein. Our data, generated based on a few representative genes, suggest that gonococcal samples positive by Aptima testing can be used to determine single nucleotide polymorphisms associated with ESC\n            <jats:sup>ds<\/jats:sup>\n            and the sequence type based on molecular strain typing. Confirmation of these findings may obviate the need for gonorrhea culture in the future.\n          <\/jats:p>","DOI":"10.1128\/jcm.01694-13","type":"journal-article","created":{"date-parts":[[2013,10,10]],"date-time":"2013-10-10T14:56:44Z","timestamp":1381417004000},"page":"4156-4160","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":6,"title":["Utility of Specimens Positive for Neisseria gonorrhoeae by the Aptima Combo 2 Assay for Assessment of Strain Diversity and Antibiotic Resistance"],"prefix":"10.1128","volume":"51","author":[{"given":"Kanti","family":"Pabbaraju","sequence":"first","affiliation":[{"name":"Provincial Laboratory for Public Health, Calgary, Alberta, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sallene","family":"Wong","sequence":"additional","affiliation":[{"name":"Provincial Laboratory for Public Health, Calgary, Alberta, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joanna J.","family":"Song","sequence":"additional","affiliation":[{"name":"Provincial Laboratory for Public Health, Calgary, Alberta, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ameeta E.","family":"Singh","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Alberta, Alberta, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ron","family":"Read","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology and Infectious Diseases, University of Calgary, Alberta, Canada"},{"name":"Department of Medicine, University of Calgary, Alberta, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Steven J.","family":"Drews","sequence":"additional","affiliation":[{"name":"Provincial Laboratory for Public Health, Calgary, Alberta, Canada"},{"name":"Department of Microbiology, Immunology and Infectious Diseases, University of Calgary, Alberta, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_1_2_2","first-page":"627","volume-title":"Sexually transmitted diseases","author":"Hook EW","year":"2008","unstructured":"HookEWIIIHandsfieldHH. 2008. Gonococcal infections in the adult, p 627\u2013645. In HolmesKSparlingPStammWPiotPWasserheitJCoreyLCohenM (ed), Sexually transmitted diseases. 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Molecular epidemiological typing within the European Gonococcal Antimicrobial Resistance Surveillance Programme reveals predominance of a multidrug-resistant clone. 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