{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T17:25:08Z","timestamp":1791307508778,"version":"4.3.1"},"reference-count":40,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2016,1,1]],"date-time":"2016-01-01T00:00:00Z","timestamp":1451606400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2016,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:named-content content-type=\"genus-species\">Staphylococcus caprae<\/jats:named-content>\n            is an emerging microorganism in human bone and joint infections (BJI). The aim of this study is to describe the features of\n            <jats:named-content content-type=\"genus-species\">S. caprae<\/jats:named-content>\n            isolates involved in BJI (H for human) compared with those of isolates recovered in goat mastitis (A for animal). Fourteen isolates of each origin were included. Identifications were performed using a Vitek 2 GP ID card,\n            <jats:italic>tuf<\/jats:italic>\n            gene sequencing, and matrix-assisted laser desorption ionization\u2013time of flight (MALDI-TOF) Vitek MS. Molecular typing was carried out using pulsed-field gel electrophoresis (PFGE) and DiversiLab technology. The crystal violet method was used to determine biofilm-forming ability. Virulence factors were searched by PCR. Vitek MS technology provides an accurate identification for the two types of isolates compared to that of gold-standard sequencing (sensitivity, 96.4%), whereas the Vitek 2 GP ID card was more effective for H isolates. Molecular typing methods revealed two distinct lineages corresponding to the origin despite few overlaps: H and A. In our experimental conditions, no significant difference was observed in biofilm production ability between H and A isolates. Nine isolates (5 H isolates and 4 A isolates) behaved as weak producers while one A isolate was a strong producer. Concerning virulence factors, the autolysin\n            <jats:italic>atlC<\/jats:italic>\n            and the serine aspartate adhesin (\n            <jats:italic>sdrZ<\/jats:italic>\n            ) genes were detected in 24 isolates (86%), whereas the lipase gene was always detected, except in one H isolate (96%). The\n            <jats:italic>ica<\/jats:italic>\n            operon was present in 23 isolates (82%). Fibrinogen-binding (\n            <jats:italic>fbe<\/jats:italic>\n            ) or collagen-binding (\n            <jats:italic>cna<\/jats:italic>\n            ) genes were not detected by using primers designed for\n            <jats:named-content content-type=\"genus-species\">Staphylococcus aureus<\/jats:named-content>\n            or\n            <jats:named-content content-type=\"genus-species\">Staphylococcus epidermidis<\/jats:named-content>\n            , even in low stringency conditions. Although\n            <jats:named-content content-type=\"genus-species\">S. caprae<\/jats:named-content>\n            probably remains underestimated in human infections, further studies are needed to better understand the evolution and the adaptation of this species to its host.\n          <\/jats:p>","DOI":"10.1128\/jcm.01696-15","type":"journal-article","created":{"date-parts":[[2015,10,29]],"date-time":"2015-10-29T02:39:06Z","timestamp":1446086346000},"page":"106-113","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":35,"title":["Characterization of Staphylococcus caprae Clinical Isolates Involved in Human Bone and Joint Infections, Compared with Goat Mastitis Isolates"],"prefix":"10.1128","volume":"54","author":[{"given":"J.","family":"d'Ersu","sequence":"first","affiliation":[{"name":"Service de Bact\u00e9riologie-Hygi\u00e8ne hospitali\u00e8re, CHU de Nantes, Nantes, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G. 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