{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T15:14:28Z","timestamp":1790781268015,"version":"4.1.0"},"reference-count":23,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2010,1,1]],"date-time":"2010-01-01T00:00:00Z","timestamp":1262304000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2010,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Chordopoxviruses of the subfamily\n            <jats:italic>Chordopoxvirinae<\/jats:italic>\n            , family\n            <jats:italic>Poxviridae<\/jats:italic>\n            , infect vertebrates and consist of at least eight genera with broad host ranges. For most chordopoxviruses, the number of viral genes and their relative order are highly conserved in the central region. The GC content of chordopoxvirus genomes, however, evolved into two distinct types: those with genome GC content of more than 60% and those with a content of less than 40% GC. Two standard PCR assays were developed to identify chordopoxviruses based on whether the target virus has a low or high GC content. In design of the assays, the genus\n            <jats:italic>Avipoxvirus<\/jats:italic>\n            , which encodes major rearrangements of gene clusters, was excluded. These pan-pox assays amplify DNA from more than 150 different isolates and strains, including from primary clinical materials, from all seven targeted genera of chordopoxviruses and four unclassified new poxvirus species. The pan-pox assays represent an important advance for the screening and diagnosis of human and animal poxvirus infections, and the technology used is accessible to many laboratories worldwide.\n          <\/jats:p>","DOI":"10.1128\/jcm.01697-09","type":"journal-article","created":{"date-parts":[[2009,11,12]],"date-time":"2009-11-12T03:17:17Z","timestamp":1257995837000},"page":"268-276","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":109,"title":["GC Content-Based Pan-Pox Universal PCR Assays for Poxvirus Detection"],"prefix":"10.1128","volume":"48","author":[{"given":"Yu","family":"Li","sequence":"first","affiliation":[{"name":"Centers for Disease Control and Prevention, National Center for Zoonotic, Vector-Borne, and Enteric Diseases, Division of Viral and Rickettsial Diseases, Poxvirus and Rabies Branch, Atlanta, Georgia 30329"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hermann","family":"Meyer","sequence":"additional","affiliation":[{"name":"Bundeswehr Institute of Microbiology, Neuherbergstrasse 11, 80937 Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Zhao","sequence":"additional","affiliation":[{"name":"Centers for Disease Control and Prevention, National Center for Zoonotic, Vector-Borne, and Enteric Diseases, Division of Viral and Rickettsial Diseases, Poxvirus and Rabies Branch, Atlanta, Georgia 30329"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Inger K.","family":"Damon","sequence":"additional","affiliation":[{"name":"Centers for Disease Control and Prevention, National Center for Zoonotic, Vector-Borne, and Enteric Diseases, Division of Viral and Rickettsial Diseases, Poxvirus and Rabies Branch, Atlanta, Georgia 30329"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"401","volume":"57","year":"2008","unstructured":"Centers for Disease Control and Prevention. 2008. 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