{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T02:47:38Z","timestamp":1771642058171,"version":"3.50.1"},"reference-count":37,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2013,11,1]],"date-time":"2013-11-01T00:00:00Z","timestamp":1383264000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2013,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>The complete 154-kbp linear double-stranded genomic DNA sequence of herpes simplex virus 2 (HSV-2), consisting of two extended regions of unique sequences bounded by a pair of inverted repeat elements, was published in 1998 and since then has been widely employed in a wide range of studies. Throughout the HSV-2 genome are scattered 150 microsatellites (also referred to as short tandem repeats) of 1- to 6-nucleotide motifs, mainly distributed in noncoding regions. Microsatellites are considered reliable markers for genetic mapping to differentiate herpesvirus strains, as shown for cytomegalovirus and HSV-1. The aim of this work was to characterize 12 polymorphic microsatellites within the HSV-2 genome by use of 3 multiplex PCR assays in combination with length polymorphism analysis for the rapid genetic differentiation of 56 HSV-2 clinical isolates and 2 HSV-2 laboratory strains (gHSV-2 and MS). This new system was applied to a specific new HSV-2 variant recently identified in HIV-1-infected patients originating from West Africa. Our results confirm that microsatellite polymorphism analysis is an accurate tool for studying the epidemiology of HSV-2 infections.<\/jats:p>","DOI":"10.1128\/jcm.01714-13","type":"journal-article","created":{"date-parts":[[2013,8,22]],"date-time":"2013-08-22T04:05:55Z","timestamp":1377144355000},"page":"3616-3623","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":20,"title":["Molecular Characterization of Herpes Simplex Virus 2 Strains by Analysis of Microsatellite Polymorphism"],"prefix":"10.1128","volume":"51","author":[{"given":"Sonia","family":"Burrel","sequence":"first","affiliation":[{"name":"UPMC University of Paris, Paris, France"},{"name":"Virology Department, Piti\u00e9-Salp\u00eatri\u00e8re University Hospital, AP-HP, Paris, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Za\u00efna","family":"Ait-Arkoub","sequence":"additional","affiliation":[{"name":"Virology Department, Piti\u00e9-Salp\u00eatri\u00e8re University Hospital, AP-HP, Paris, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Delphine","family":"Voujon","sequence":"additional","affiliation":[{"name":"Virology Department, Piti\u00e9-Salp\u00eatri\u00e8re University Hospital, AP-HP, Paris, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claire","family":"Deback","sequence":"additional","affiliation":[{"name":"INSERM UMR-S996, Clamart, France"},{"name":"Virology Department, Paul Brousse University Hospital, AP-HP, Villejuif, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emiliana P.","family":"Abrao","sequence":"additional","affiliation":[{"name":"UPMC University of Paris, Paris, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Henri","family":"Agut","sequence":"additional","affiliation":[{"name":"UPMC University of Paris, Paris, France"},{"name":"Virology Department, Piti\u00e9-Salp\u00eatri\u00e8re University Hospital, AP-HP, Paris, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Boutolleau","sequence":"additional","affiliation":[{"name":"UPMC University of Paris, Paris, France"},{"name":"Virology Department, Piti\u00e9-Salp\u00eatri\u00e8re University Hospital, AP-HP, Paris, France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1136\/sti.2006.020966"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(07)61908-4"},{"key":"e_1_3_2_4_2","volume-title":"Human herpesviruses: biology, therapy, and immunoprophylaxis","author":"Whitley R","year":"2007","unstructured":"WhitleyR KimberlinDW ProberCG. 2007. Pathogenesis and disease, chapter 32. In ArvinA Campadelli-FiumeG MocarskiE MoorePS RoizmanB WhitleyR YamanishiK (ed), Human herpesviruses: biology, therapy, and immunoprophylaxis. Cambridge University Press, Cambridge, United Kingdom. http:\/\/www.ncbi.nlm.nih.gov\/books\/NBK47449\/."},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.72.11.4243"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-68-1-19"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/140.3.295"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.38.3.834-842.1982"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/149.4.645"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2004.01.013"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-0934(02)00264-1"},{"key":"e_1_3_2_12_2","volume-title":"Human herpesviruses: biology, therapy, and immunoprophylaxis","author":"Baines J","year":"2007","unstructured":"BainesJ PellettP. 2007. Genetic comparison of human alphaherpesvirus genomes, chapter 5. In ArvinA Campadelli-FiumeG MocarskiE MoorePS RoizmanB WhitleyR YamanishiK (ed), Human herpesviruses: biology, therapy, and immunoprophylaxis. Cambridge University Press, Cambridge, United Kingdom. http:\/\/www.ncbi.nlm.nih.gov\/books\/NBK47393\/."},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002282"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1007\/s004120000089"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1093\/bib\/bbn028"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrg1348"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/131.2.471"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2009.08.004"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01565-08"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.6.2219-2226.2001"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2013.03.008"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00669-10"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.antiviral.2005.11.004"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.73.8.6265-6270.1999"},{"key":"e_1_3_2_25_2","first-page":"365","article-title":"Primer3 on the WWW for general users and for biologist programmers","volume":"132","author":"Rozen S","year":"2000","unstructured":"RozenS SkaletskyH. 2000. Primer3 on the WWW for general users and for biologist programmers. Methods Mol. Biol. 132:365\u2013386.","journal-title":"Methods Mol. Biol."},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2334-2-7"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1386-6532(02)00263-9"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1038\/74238"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.69.4.2697-2699.1995"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-1702(97)01468-8"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002282"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2010.01.019"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0913586107"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20101402"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00615-10"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.73.12.9796-9802.1999"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00054-06"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02300753"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.01714-13","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.01714-13","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T11:31:49Z","timestamp":1646479909000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.01714-13"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,11]]},"references-count":37,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2013,11]]}},"alternative-id":["10.1128\/JCM.01714-13"],"URL":"https:\/\/doi.org\/10.1128\/jcm.01714-13","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,11]]},"assertion":[{"value":"2013-07-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-08-16","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-10-16","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}