{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T06:59:54Z","timestamp":1791356394168,"version":"4.3.1"},"reference-count":30,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2015,11,1]],"date-time":"2015-11-01T00:00:00Z","timestamp":1446336000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2015,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Members of the\n            <jats:named-content content-type=\"genus-species\">Mycobacterium abscessus<\/jats:named-content>\n            group (MAG) cause lung, soft tissue, and disseminated infections. The oral macrolides clarithromycin and azithromycin are commonly used for treatment. MAG can display clarithromycin resistance through the inducible\n            <jats:italic>erm<\/jats:italic>\n            (41) gene or via acquired mutations in the\n            <jats:italic>rrl<\/jats:italic>\n            (23S rRNA) gene. Strains harboring a truncation or a T28C substitution in\n            <jats:italic>erm<\/jats:italic>\n            (41) lose the inducible resistance trait. Phenotypic detection of clarithromycin resistance requires extended incubation (14 days), highlighting the need for faster methods to detect resistance. Two real-time PCR-based assays were developed to assess inducible and acquired clarithromycin resistance and tested on a total of 90 clinical and reference strains. A SYBR green assay was designed to distinguish between a full-length and truncated\n            <jats:italic>erm<\/jats:italic>\n            (41) gene by temperature shift in melting curve analysis. Single nucleotide polymorphism (SNP) allele discrimination assays were developed to distinguish T or C at position 28 of\n            <jats:italic>erm<\/jats:italic>\n            (41) and 23S rRNA\n            <jats:italic>rrl<\/jats:italic>\n            gene mutations at position 2058 and\/or 2059. Truncated and full-size\n            <jats:italic>erm<\/jats:italic>\n            (41) genes were detected in 21\/90 and 69\/90 strains, respectively, with 64\/69 displaying T at nucleotide position 28 and 5\/69 containing C at that position. Fifteen isolates showed\n            <jats:italic>rrl<\/jats:italic>\n            mutations conferring clarithromycin resistance, including A2058G (11 isolates), A2058C (3 isolates), and A2059G (1 isolate). Targeted sequencing and phenotypic assessment of resistance concurred with molecular assay results. Interestingly, we also noted cooccurring strains harboring an active\n            <jats:italic>erm<\/jats:italic>\n            (41), inactive\n            <jats:italic>erm<\/jats:italic>\n            (41), and\/or acquired mutational resistance, as well as slowly growing MAG strains and also strains displaying an inducible resistance phenotype within 5 days, long before the recommended 14-day extended incubation.\n          <\/jats:p>","DOI":"10.1128\/jcm.01714-15","type":"journal-article","created":{"date-parts":[[2015,8,13]],"date-time":"2015-08-13T02:29:43Z","timestamp":1439432983000},"page":"3430-3437","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":31,"title":["New Real-Time PCR Assays for Detection of Inducible and Acquired Clarithromycin Resistance in the Mycobacterium abscessus Group"],"prefix":"10.1128","volume":"53","author":[{"given":"Shamira J.","family":"Shallom","sequence":"first","affiliation":[{"name":"Microbiology Service, Department of Laboratory Medicine, Clinical Center, National Institutes of Health, Bethesda, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalia S.","family":"Moura","sequence":"additional","affiliation":[{"name":"Microbiology Service, Department of Laboratory Medicine, Clinical Center, National Institutes of Health, Bethesda, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenneth N.","family":"Olivier","sequence":"additional","affiliation":[{"name":"Cardiovascular and Pulmonary Branch, NHLBI, National Institutes of Health, Bethesda, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth P.","family":"Sampaio","sequence":"additional","affiliation":[{"name":"Laboratory of Clinical Infectious Diseases, NIAID, National Institutes of Health, Bethesda, Maryland, USA"},{"name":"Leprosy Laboratory, Oswaldo Cruz Institute, FIOCRUZ, Rio de Janeiro, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven M.","family":"Holland","sequence":"additional","affiliation":[{"name":"Laboratory of Clinical Infectious Diseases, NIAID, National Institutes of Health, Bethesda, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adrian M.","family":"Zelazny","sequence":"additional","affiliation":[{"name":"Microbiology Service, Department of Laboratory Medicine, Clinical Center, National Institutes of Health, Bethesda, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.023770-0"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(13)60632-7"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid2003.131106"},{"key":"e_1_3_3_5_2","first-page":"835","article-title":"Nontuberculous mycobacteria. II","volume":"167","author":"Olivier KN","year":"2003","unstructured":"Olivier KN, Weber DJ, Lee JH, Handler A, Tudor G, Molina PL, Tomashefski J, Knowles MR. 2003. Nontuberculous mycobacteria. II. Nested-cohort study of impact on cystic fibrosis lung disease. Am J Respir Crit Care Med 167:835\u2013840.","journal-title":"Nested-cohort study of impact on cystic fibrosis lung disease. Am J Respir Crit Care Med"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.2217\/fmb.10.49"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1097\/QCO.0000000000000104"},{"key":"e_1_3_3_8_2","doi-asserted-by":"crossref","unstructured":"Brown BA Wallace RJ Jr Onyi GO De Rosas V Wallace RJ III. 1992. Activities of four macrolides including clarithromycin against Mycobacterium fortuitum Mycobacterium chelonae and M. chelonae-like organisms Antimicrob Agents Chemother 36:180\u2013184.","DOI":"10.1128\/AAC.36.1.180"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.200604-571ST"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01275-08"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1348-0421.2010.00221.x"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00861-10"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1997.5811946.x"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2004.07.095"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0501598102"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01398-10"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dku007"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0038420"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-011-1510-9"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.3.1051-1058.2005"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-13-3"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01274-10"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01688-08"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01132-13"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-14-436"},{"key":"e_1_3_3_26_2","first-page":"14","volume-title":"CLSI document M24-A2","author":"CLSI","year":"2011","unstructured":"CLSI. 2011. Susceptibility testing of mycobacteria, nocardiae, and other aerobic actinomycetes; approved standard, 2nd ed, p 14. CLSI document M24-A2. Clinical and Laboratory Standards Institute, Wayne, PA.","edition":"2"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/20.5.1441"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.40.7.1676"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02950-14"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dks279"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcla.21702"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.01714-15","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.01714-15","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T08:45:05Z","timestamp":1646469905000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.01714-15"}},"subtitle":[],"editor":[{"given":"G. A.","family":"Land","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2015,11]]},"references-count":30,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2015,11]]}},"alternative-id":["10.1128\/JCM.01714-15"],"URL":"https:\/\/doi.org\/10.1128\/jcm.01714-15","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,11]]},"assertion":[{"value":"2015-06-29","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-08-01","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-10-16","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}