{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T01:44:45Z","timestamp":1778809485294,"version":"3.51.4"},"reference-count":27,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2012,12,1]],"date-time":"2012-12-01T00:00:00Z","timestamp":1354320000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2012,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The Sensititre YeastOne (SYO) method is a widely used method to determine the susceptibility of\n            <jats:named-content content-type=\"genus-species\">Candida<\/jats:named-content>\n            spp. to antifungal agents. CLSI clinical breakpoints (CBP) have been reported for antifungals, but not using this method. In the absence of CBP, epidemiological cutoff values (ECVs) are useful to separate wild-type (WT) isolates (those without mechanisms of resistance) from non-WT isolates (those that can harbor some resistance mechanisms), which is the goal of any susceptibility test. The ECVs for five agents, obtained using the MIC distributions determined by the SYO test, were calculated and contrasted with those for three statistical methods and the MIC\n            <jats:sub>50<\/jats:sub>\n            and modal MIC, both plus 2-fold dilutions. The median ECVs (in mg\/liter) (% of isolates inhibited by MICs equal to or less than the ECV; number of isolates tested) of the five methods for anidulafungin, micafungin, caspofungin, amphotericin B, and flucytosine, respectively, were as follows: 0.25 (98.5%; 656), 0.06 (95.1%; 659), 0.25 (98.7%; 747), 2 (100%; 923), and 1 (98.5%; 915) for\n            <jats:named-content content-type=\"genus-species\">Candida albicans<\/jats:named-content>\n            ; 8 (100%; 352), 4 (99.2%; 392), 2 (99.2%; 480), 1 (99.8%; 603), and 0.5 (97.9%; 635) for\n            <jats:named-content content-type=\"genus-species\">C. parapsilosis<\/jats:named-content>\n            ; 1 (99.2%; 123), 0.12 (99.2%; 121), 0.25 (99.2%; 138), 2 (100%; 171), and 0.5 (97.2%; 175) for\n            <jats:named-content content-type=\"genus-species\">C. tropicalis<\/jats:named-content>\n            ; 0.12 (96.6%; 174), 0.06 (96%; 176), 0.25 (98.4%; 188), 2 (100%; 209), and 0.25 (97.6%; 208) for\n            <jats:named-content content-type=\"genus-species\">C. glabrata<\/jats:named-content>\n            ; 0.25 (97%; 33), 0.5 (93.9%; 33), 1 (91.9%; 37), 4 (100%; 51), and 32 (100%; 53) for\n            <jats:named-content content-type=\"genus-species\">C. krusei<\/jats:named-content>\n            ; and 4 (100%; 33), 2 (100%; 33), 2 (100%; 54), 1 (100%; 90), and 0.25 (93.4%; 91) for\n            <jats:named-content content-type=\"genus-species\">C. orthopsilosis<\/jats:named-content>\n            . The three statistical methods gave similar ECVs (within one dilution) and included \u226595% of isolates. These tentative ECVs would be useful for monitoring the emergence of isolates with reduced susceptibility by use of the SYO method.\n          <\/jats:p>","DOI":"10.1128\/jcm.01730-12","type":"journal-article","created":{"date-parts":[[2012,9,27]],"date-time":"2012-09-27T12:07:52Z","timestamp":1348747672000},"page":"3921-3926","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":46,"title":["Comparison of Three Statistical Methods for Establishing Tentative Wild-Type Population and Epidemiological Cutoff Values for Echinocandins, Amphotericin B, Flucytosine, and Six Candida Species as Determined by the Colorimetric Sensititre YeastOne Method"],"prefix":"10.1128","volume":"50","author":[{"given":"Emilia","family":"Cant\u00f3n","sequence":"first","affiliation":[{"name":"Unidad de Microbiolog\u00eda Experimental, Centro de Investigaci\u00f3n, Hospital La Fe, Valencia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Javier","family":"Pem\u00e1n","sequence":"additional","affiliation":[{"name":"Servicio de Microbiolog\u00eda, Hospital La Fe, Valencia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Herv\u00e1s","sequence":"additional","affiliation":[{"name":"Unidad Bioestadistica, IIS La Fe, Valencia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carmen","family":"I\u00f1iguez","sequence":"additional","affiliation":[{"name":"\u00c1rea de Ambiente y Salud, Centro Investigaci\u00f3n Salud P\u00fablica, Valencia"},{"name":"Universidad de Valencia, Valencia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Navarro","sequence":"additional","affiliation":[{"name":"Servicio de Microbiolog\u00eda, Hospital Cl\u00ednico, Valencia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julia","family":"Echeverr\u00eda","sequence":"additional","affiliation":[{"name":"Servicio de Microbiolog\u00eda, Hospital Donostia, San Sebasti\u00e1n"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9","family":"Mart\u00ednez-Alarc\u00f3n","sequence":"additional","affiliation":[{"name":"Servicio de Microbiolog\u00eda, Hospital General, Ciudad Real"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dionisia","family":"Fontanals","sequence":"additional","affiliation":[{"name":"Servicio de Microbiolog\u00eda, Corporaci\u00f3 Sanitari Parc Tauli, Sabadell"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B\u00e1rbara","family":"Gomila-Sard","sequence":"additional","affiliation":[{"name":"Servicio de Microbiolog\u00eda, Hospital General, Castell\u00f3n"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Buenaventura","family":"Buend\u00eda","sequence":"additional","affiliation":[{"name":"Servicio de Microbiolog\u00eda, Hospital Universitario La Princesa, Madrid"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luis","family":"Torroba","sequence":"additional","affiliation":[{"name":"Servicio de Microbiolog\u00eda, Hospital Virgen del Camino, Pamplona"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Josefina","family":"Ayats","sequence":"additional","affiliation":[{"name":"Servicio de Microbiolog\u00eda, Hospital Universitari de Bellvitge, Hospitalet de Llobregat"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Angel","family":"Bratos","sequence":"additional","affiliation":[{"name":"Servicio de Microbiolog\u00eda, Hospital Cl\u00ednico, Valladolid"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ferran","family":"S\u00e1nchez-Reus","sequence":"additional","affiliation":[{"name":"Servicio de Microbiolog\u00eda, Hospital de la Santa Creu i Sant Pau, Barcelona"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Isabel","family":"Fern\u00e1ndez-Natal","sequence":"additional","affiliation":[{"name":"Servicio de Microbiolog\u00eda, Complejo Asistencial Universitario, Le\u00f3n, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"name":"the FUNGEMYCA Study Group","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01840-06"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01256-09"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.49.4.1604-1607.2005"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00466-11"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.41.11.2471"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00686-11"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.2.718-721.2004"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1198\/016214502760047131"},{"key":"e_1_3_2_10_2","unstructured":"FraleyC\nRafteryAE\nMurphyTB\nScruccaL. 2012. MCLUST version 4 for R: normal mixture modeling for model-based clustering classification and density estimation. Technical report no. 597. Department of Statistics University of Washington Seattle WA. http:\/\/www.stat.washington.edu\/research\/reports\/2012\/tr597.pdf."},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00508-11"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkg312"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01101-10"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.64.8.3244-3251.1996"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.05959-11"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1086\/596757"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dks019"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1080\/13693780701218689"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.05047-11"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02161-10"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01590-09"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00493-08"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.drup.2011.01.004"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00248-12"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1086\/513640"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00156-08"},{"key":"e_1_3_2_27_2","volume-title":"Monographs on statistics and applied probability.","author":"Silverman BW","year":"1986","unstructured":"SilvermanBW. 1986. Density estimation for statistics and data analysis. Monographs on statistics and applied probability. Chapman and Hall, London, United Kingdom."},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2006.01377.x"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.01730-12","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.01730-12","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T06:06:12Z","timestamp":1646460372000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.01730-12"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,12]]},"references-count":27,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2012,12]]}},"alternative-id":["10.1128\/JCM.01730-12"],"URL":"https:\/\/doi.org\/10.1128\/jcm.01730-12","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,12]]},"assertion":[{"value":"2012-07-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2012-09-15","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2012-11-16","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}