{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T21:00:57Z","timestamp":1788469257160,"version":"build-2803163510"},"reference-count":28,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2014,1,1]],"date-time":"2014-01-01T00:00:00Z","timestamp":1388534400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2014,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            We aimed to compare sputum and nasopharyngeal aspirate (NpA) samples and the PCR gene targets\n            <jats:italic>lytA<\/jats:italic>\n            and Spn9802 in quantitative PCR (qPCR) assays for rapid detection of pneumococcal etiology in community-acquired pneumonia (CAP). Seventy-eight adult patients hospitalized for radiologically confirmed CAP had both good-quality sputum and NpA specimens collected at admission. These samples were subjected to\n            <jats:italic>lytA<\/jats:italic>\n            qPCR and Spn9802 qPCR assays with analytical times of &lt;3 h. Thirty-two patients had CAP with a pneumococcal etiology, according to conventional diagnostic criteria. The following qPCR positivity rates were noted in CAP cases with and without pneumococcal etiology: 96% and 15% (sputum\n            <jats:italic>lytA<\/jats:italic>\n            assay), 96% and 17% (sputum Spn9802 assay), 81% and 11% (NpA\n            <jats:italic>lytA<\/jats:italic>\n            assay), and 81% and 20% (NpA Spn9802 assay), respectively. The mean\n            <jats:italic>lytA<\/jats:italic>\n            and Spn9802 DNA levels were significantly higher in qPCR-positive sputum samples from cases with pneumococcal etiology than in qPCR-positive sputum samples from CAP cases without pneumococcal etiology or qPCR-positive NpA samples from cases with pneumococcal etiology (\n            <jats:italic>P<\/jats:italic>\n            &lt; 0.02 for all comparisons). For detection of pneumococcal etiology, receiver operating characteristic curve analysis showed that sputum specimens were superior to NpA specimens as the sample type (\n            <jats:italic>P<\/jats:italic>\n            &lt; 0.02 for both gene targets) and\n            <jats:italic>lytA<\/jats:italic>\n            tended to be superior to Spn9802 as the gene target. The best-performing test, the sputum\n            <jats:italic>lytA<\/jats:italic>\n            qPCR assay, showed high sensitivity (94%) and specificity (96%) with a cutoff value of 10\n            <jats:sup>5<\/jats:sup>\n            DNA copies\/ml. In CAP patients with good sputum production, this test has great potential to be used for the rapid detection of pneumococcal etiology and to target penicillin therapy.\n          <\/jats:p>","DOI":"10.1128\/jcm.01742-13","type":"journal-article","created":{"date-parts":[[2013,10,24]],"date-time":"2013-10-24T03:09:23Z","timestamp":1382584163000},"page":"83-89","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":60,"title":["Comparison of Sputum and Nasopharyngeal Aspirate Samples and of the PCR Gene Targets\n            <i>lytA<\/i>\n            and Spn9802 for Quantitative PCR for Rapid Detection of Pneumococcal Pneumonia"],"prefix":"10.1128","volume":"52","author":[{"given":"Kristoffer","family":"Str\u00e5lin","sequence":"first","affiliation":[{"name":"Department of Infectious Diseases, Karolinska University Hospital, Stockholm, Sweden"},{"name":"Department of Infectious Diseases, \u00d6rebro University Hospital, \u00d6rebro, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bj\u00f6rn","family":"Herrmann","sequence":"additional","affiliation":[{"name":"Section of Clinical Bacteriology, Department of Medical Sciences, Uppsala University, Uppsala, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guma","family":"Abdeldaim","sequence":"additional","affiliation":[{"name":"Section of Clinical Bacteriology, Department of Medical Sciences, Uppsala University, Uppsala, Sweden"},{"name":"Department of Microbiology, Faculty of Medicine, Benghazi University, Benghazi, Libya"},{"name":"Department of Clinical Mycobacteriology, National Center for Diseases Control, Benghazi, Libya"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Per","family":"Olc\u00e9n","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine\/Clinical Microbiology, \u00d6rebro University Hospital, \u00d6rebro, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hans","family":"Holmberg","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, \u00d6rebro University Hospital, \u00d6rebro, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paula","family":"M\u00f6lling","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine\/Clinical Microbiology, \u00d6rebro University Hospital, \u00d6rebro, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.2471\/BLT.11.088435"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMp1215093"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1001\/archinternmed.2010.474"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.3109\/00365548.2012.700120"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.02.00702002"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1086\/648678"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0060273"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1086\/499596"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1086\/429919"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1001\/archinternmed.2010.347"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01636-09"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2009.03069.x"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cir859"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2007.08.010"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.9.4528-4534.2005"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2009.02714.x"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02498-06"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.7.3221-3226.2005"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2006.01.007"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.044107-0"},{"key":"e_1_3_2_22_2","volume-title":"Abstr. 8th Int. 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Pneumococci Pneumococcal Dis., Iguacu Falls, Brazil."}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.01742-13","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.01742-13","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T11:49:59Z","timestamp":1646480999000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.01742-13"}},"subtitle":[],"editor":[{"given":"E.","family":"Munson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2014,1]]},"references-count":28,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2014,1]]}},"alternative-id":["10.1128\/JCM.01742-13"],"URL":"https:\/\/doi.org\/10.1128\/jcm.01742-13","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,1]]},"assertion":[{"value":"2013-07-03","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-10-16","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-12-20","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}