{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T15:31:36Z","timestamp":1779377496521,"version":"3.53.1"},"reference-count":37,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2016,4,1]],"date-time":"2016-04-01T00:00:00Z","timestamp":1459468800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"National Scientific Foundation Committee","award":["81172801"],"award-info":[{"award-number":["81172801"]}]},{"name":"973 Program from Chinese Ministry of Science and Technology","award":["2013CB531600"],"award-info":[{"award-number":["2013CB531600"]}]},{"name":"International Science & Technology Cooperation Programme of China","award":["2015DFR31060"],"award-info":[{"award-number":["2015DFR31060"]}]},{"name":"National Scientific Foundation Committee","award":["81273229"],"award-info":[{"award-number":["81273229"]}]},{"name":"National Key Program for Infectious Diseases of China","award":["2013ZX10004804-007"],"award-info":[{"award-number":["2013ZX10004804-007"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2016,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:named-content content-type=\"genus-species\">Aspergillus fumigatus<\/jats:named-content>\n            is a conditional pathogen and the major cause of life-threatening invasive aspergillosis (IA) in immunocompromised patients. The early and rapid detection of\n            <jats:named-content content-type=\"genus-species\">A. fumigatus<\/jats:named-content>\n            infection is still a major challenge. In this study, the new member of the fungal annexin family, annexin C4, was chosen as the target to design a loop-mediated isothermal amplification (LAMP) assay for the rapid, specific, and sensitive detection of\n            <jats:named-content content-type=\"genus-species\">A. fumigatus<\/jats:named-content>\n            . The evaluation of the specificity of the LAMP assay that was developed showed that no false-positive results were observed for the 22 non-\n            <jats:named-content content-type=\"genus-species\">A. fumigatus<\/jats:named-content>\n            strains, including 5 species of the\n            <jats:named-content content-type=\"genus-species\">Aspergillus<\/jats:named-content>\n            genus. Its detection limit was approximately 10 copies per reaction in reference plasmids, with higher sensitivity than that of real-time quantitative PCR (qPCR) at 10\n            <jats:sup>2<\/jats:sup>\n            copies for the same target. Clinical samples from a total of 69 patients with probable IA (\n            <jats:italic>n =<\/jats:italic>\n            14) and possible IA (\n            <jats:italic>n<\/jats:italic>\n            = 55) were subjected to the LAMP assay, and positive results were found for the 14 patients with probable IA (100%) and 34 patients with possible IA (61.82%). When detection using the LAMP assay was compared with that using qPCR in the 69 clinical samples, the LAMP assay demonstrated a sensitivity of 89.19% and the concordance rate for the two methods was 72.46%. Accordingly, we report that a valuable LAMP assay for the rapid, specific, and simple detection of\n            <jats:named-content content-type=\"genus-species\">A. fumigatus<\/jats:named-content>\n            in clinical testing has been developed.\n          <\/jats:p>","DOI":"10.1128\/jcm.01751-15","type":"journal-article","created":{"date-parts":[[2016,1,21]],"date-time":"2016-01-21T04:34:55Z","timestamp":1453350895000},"page":"950-955","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":24,"title":["Development and Evaluation of a Loop-Mediated Isothermal Amplification Method for Rapid Detection of Aspergillus fumigatus"],"prefix":"10.1128","volume":"54","author":[{"given":"Qing","family":"Tang","sequence":"first","affiliation":[{"name":"Key Laboratory of Bio-resources and Eco-environment of the Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, Sichuan, People's Republic of 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