{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T04:11:08Z","timestamp":1791951068921,"version":"4.3.4"},"reference-count":19,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2007,2,1]],"date-time":"2007-02-01T00:00:00Z","timestamp":1170288000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2007,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The protozoan parasite\n            <jats:italic>Babesia bovis<\/jats:italic>\n            , a reemerging threat to U.S. cattle, is acquired by adult female ticks of the subgenus\n            <jats:italic>Boophilus<\/jats:italic>\n            and is transovarially transmitted as the kinete stage to developing larval offspring. Sporozoites develop within larvae and are transmitted during larval feeding on a bovine host. This study evaluated the efficiency of\n            <jats:italic>B. bovis<\/jats:italic>\n            infection within\n            <jats:italic>Rhipicephalus<\/jats:italic>\n            (\n            <jats:italic>Boophilus<\/jats:italic>\n            )\n            <jats:italic>microplus<\/jats:italic>\n            following acquisition feeding on acutely parasitemic cattle. Parasite levels were quantified in blood from experimentally infected cattle and within hemolymph and larvae derived from acquisition-fed female\n            <jats:italic>B. microplus<\/jats:italic>\n            . There was a positive correlation between blood parasite levels in acutely parasitemic cattle and kinete levels in the hemolymph of adult female\n            <jats:italic>Boophilus<\/jats:italic>\n            ticks following acquisition feeding; however, there was no relationship between kinete levels in females and infection rates of larval progeny.\n            <jats:italic>Boophilus microplus<\/jats:italic>\n            females that acquisition fed produced larval progeny with infection rates of 12% to 48%. Importantly, larvae derived from replete females with very low levels of kinete infection, as demonstrated by microscopy and PCR, had infection rates of 22% to 30% and transmitted\n            <jats:italic>B. bovis<\/jats:italic>\n            during transmission feeding. These data demonstrate that although hemolymph infection may be undetectable, transmission to larval progeny occurs at a level which ensures transmission to the bovine host.\n          <\/jats:p>","DOI":"10.1128\/jcm.01757-06","type":"journal-article","created":{"date-parts":[[2006,12,14]],"date-time":"2006-12-14T02:55:43Z","timestamp":1166064943000},"page":"426-431","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":70,"title":["Transovarial Transmission Efficiency of\n            <i>Babesia bovis<\/i>\n            Tick Stages Acquired by\n            <i>Rhipicephalus<\/i>\n            (\n            <i>Boophilus<\/i>\n            )\n            <i>microplus<\/i>\n            during Acute Infection"],"prefix":"10.1128","volume":"45","author":[{"given":"Jeanne M.","family":"Howell","sequence":"first","affiliation":[{"name":"Program in Vector-Borne Diseases, Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164-7040"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Massaro W.","family":"Ueti","sequence":"additional","affiliation":[{"name":"Program in Vector-Borne Diseases, Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164-7040"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guy H.","family":"Palmer","sequence":"additional","affiliation":[{"name":"Program in Vector-Borne Diseases, Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164-7040"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Glen A.","family":"Scoles","sequence":"additional","affiliation":[{"name":"Animal Disease Research Unit, USDA Agricultural Research Service, Pullman, Washington 99164-6630"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donald P.","family":"Knowles","sequence":"additional","affiliation":[{"name":"Program in Vector-Borne Diseases, Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164-7040"},{"name":"Animal Disease Research Unit, USDA Agricultural Research Service, Pullman, Washington 99164-6630"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182004005190"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00539454"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0034-5288(95)90119-1"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.34.11.2748-2755.1996"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0020-7519(82)90035-2"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1603\/0022-2585-39.5.770"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1603\/0022-2585-40.4.482"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1603\/0022-2585-41.2.193"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1080\/00034983.1971.11686759"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0034-5288(18)33242-9"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1603\/0022-2585(2005)042[0912:FROOBM]2.0.CO;2"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.11.6448-6455.2002"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.3.1599-1603.2002"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.8.3921-3928.1999"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2005.03.007"},{"key":"e_1_3_2_17_2","unstructured":"Pelzel A. August 2005. Cattle fever tick surveillance in Texas. NAHSS Outlook [Online.] http:\/\/www.aphis.usda.gov\/vs\/ceah\/ncahs\/nsu\/outlook\/issue7\/cattle_fever_tick_surveillance.pdf\n."},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1093\/jee\/91.1.153"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.12.6865-6870.2000"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/0020-7519(72)90008-2"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.01757-06","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.01757-06","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T05:44:26Z","timestamp":1646459066000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.01757-06"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,2]]},"references-count":19,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2007,2]]}},"alternative-id":["10.1128\/JCM.01757-06"],"URL":"https:\/\/doi.org\/10.1128\/jcm.01757-06","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,2]]},"assertion":[{"value":"2006-08-24","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2006-12-04","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2007-02-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}