{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T12:56:32Z","timestamp":1792673792628,"version":"4.5.0"},"reference-count":18,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2011,2,1]],"date-time":"2011-02-01T00:00:00Z","timestamp":1296518400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2011,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Enterobacterial isolates expressing the carbapenemase NDM-1 are emerging worldwide. Twenty-seven NDM-1-positive isolates of worldwide origin were included in this study to identify these strains as not only pathogens but also colonizers of normal flora for infection control screening. Although susceptibility to carbapenems varied, a combined test (IMP\/IMP + EDTA), the Etest MBL, and automated susceptibility testing by Vitek2 (bioM\u00e9rieux) identified those NDM-1 producers as verified by PCR using specific primers. Screening for carriers of NDM-1 producers may be based on media such as the ChromID ESBL culture medium routinely used to screen for extended-spectrum \u03b2-lactamase producers, which gives excellent detection levels with low limits of detection ranging from 8 \u00d7 10\n            <jats:sup>0<\/jats:sup>\n            to 5 \u00d7 10\n            <jats:sup>2<\/jats:sup>\n            CFU\/ml. The CHROMagar KPC culture medium had higher limits of detection (1 \u00d7 10\n            <jats:sup>1<\/jats:sup>\n            to 5 \u00d7 10\n            <jats:sup>5<\/jats:sup>\n            CFU\/ml) and may be proposed for the follow-up of outbreaks of infections with NDM-1 producers. Colonies growing on these screening media can be verified as NDM-1 producers with molecular methods as described herein.\n          <\/jats:p>","DOI":"10.1128\/jcm.01773-10","type":"journal-article","created":{"date-parts":[[2010,12,2]],"date-time":"2010-12-02T05:50:43Z","timestamp":1291269043000},"page":"718-721","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":285,"title":["How To Detect NDM-1 Producers"],"prefix":"10.1128","volume":"49","author":[{"given":"Patrice","family":"Nordmann","sequence":"first","affiliation":[{"name":"Service de Bact\u00e9riologie-Virologie, INSERM U914 Emerging Resistance to Antibiotics, H\u00f4pital de Bic\u00eatre, Assistance Publique\/H\u00f4pitaux de Paris, Facult\u00e9 de M\u00e9decine Paris Sud, Le Kremlin-Bic\u00eatre, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurent","family":"Poirel","sequence":"additional","affiliation":[{"name":"Service de Bact\u00e9riologie-Virologie, INSERM U914 Emerging Resistance to Antibiotics, H\u00f4pital de Bic\u00eatre, Assistance Publique\/H\u00f4pitaux de Paris, Facult\u00e9 de M\u00e9decine Paris Sud, Le Kremlin-Bic\u00eatre, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Am\u00e9lie","family":"Carr\u00ebr","sequence":"additional","affiliation":[{"name":"Service de Bact\u00e9riologie-Virologie, INSERM U914 Emerging Resistance to Antibiotics, H\u00f4pital de Bic\u00eatre, Assistance Publique\/H\u00f4pitaux de Paris, Facult\u00e9 de M\u00e9decine Paris Sud, Le Kremlin-Bic\u00eatre, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark A.","family":"Toleman","sequence":"additional","affiliation":[{"name":"Department of Immunology, Immunity and Biochemistry, School of Medicine, Cardiff University, Cardiff, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timothy R.","family":"Walsh","sequence":"additional","affiliation":[{"name":"Department of Immunology, Immunity and Biochemistry, School of Medicine, Cardiff University, Cardiff, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02277-09"},{"key":"e_1_3_2_3_2","first-page":"256","article-title":"Guidance for control of infections with carbapenem-resistant or carbapenemase-producing Enterobacteriaceae in acute care facilities","volume":"58","author":"Center for Disease Control and Prevention","year":"2009","unstructured":"Center for Disease Control and Prevention. 2009. Guidance for control of infections with carbapenem-resistant or carbapenemase-producing Enterobacteriaceae in acute care facilities. Morb. Mortal. Wkly. Rep. 58:256\u2013266.","journal-title":"Morb. Mortal. Wkly. Rep"},{"key":"e_1_3_2_4_2","first-page":"750","article-title":"Detection of Enterobacteriaceae isolates carrying metallo-beta-lactamase\u2014United States","volume":"25","author":"Centers for Disease Control and Prevention","year":"2010","unstructured":"Centers for Disease Control and Prevention. 2010. Detection of Enterobacteriaceae isolates carrying metallo-beta-lactamase\u2014United States. Morb. Mortal. Wkly. Rep. 25:750.","journal-title":"Morb. Mortal. Wkly. Rep"},{"key":"e_1_3_2_5_2","unstructured":"Clinical and Laboratory Standards Institute. 2010. Performance standards for antimicrobial susceptibility testing. CLSI M100-S20U. Update June 2010. Clinical and Laboratory Standards Institute Wayne PA."},{"key":"e_1_3_2_6_2","first-page":"147","article-title":"New Delhi Metallo-beta lactamase (NDM-1) in Enterobacteriaceae: treatment options with carbapenems compromised","volume":"58","author":"Deshpande P.","year":"2010","unstructured":"DeshpandeP.. 2010. New Delhi Metallo-beta lactamase (NDM-1) in Enterobacteriaceae: treatment options with carbapenems compromised. J. Assoc. Physicians India 58:147\u2013149.","journal-title":"J. Assoc. 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