{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T21:07:03Z","timestamp":1780088823080,"version":"3.54.0"},"reference-count":30,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2007,1,1]],"date-time":"2007-01-01T00:00:00Z","timestamp":1167609600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2007,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:italic>Coccidioides<\/jats:italic>\n            spp. are dimorphic fungal pathogens endemic to the semiarid regions of North, Central, and South America. Currently, direct smear and culture are the most common means of identifying\n            <jats:italic>Coccidioides<\/jats:italic>\n            spp. While these methods offer relatively sensitive and specific means of detecting\n            <jats:italic>Coccidioides<\/jats:italic>\n            spp., growth in culture may take up to 3 weeks, potentially delaying the diagnosis and initiation of appropriate antifungal therapy. In addition, growth of the organism represents a significant safety risk to laboratory personnel. The need for a rapid and safe means of diagnosing coccidioidomycosis prompted us to develop a real-time PCR assay to detect\n            <jats:italic>Coccidioides<\/jats:italic>\n            spp. directly from clinical specimens. Primers and fluorescent resonance energy transfer (FRET) probes were designed to target the internal transcribed spacer 2 region of\n            <jats:italic>Coccidioides<\/jats:italic>\n            . The assay's limit of detection is below 50 targets per reaction. An analysis of 40\n            <jats:italic>Coccidioides<\/jats:italic>\n            sp. clinical isolates grown in culture demonstrated 100% sensitivity of the assay. A cross-reactivity panel containing fungi, bacteria, mycobacteria, and viruses was tested and demonstrated 100% specificity for\n            <jats:italic>Coccidioides<\/jats:italic>\n            spp. An analysis of 266 respiratory specimens by LightCycler PCR demonstrated 100% sensitivity and 98.4% specificity for\n            <jats:italic>Coccidioides<\/jats:italic>\n            spp. compared with culture. Analysis of 66 fresh tissue specimens yielded 92.9% sensitivity and 98.1% specificity versus those of the culture method. The sensitivity of the assay testing 148 paraffin-embedded tissue samples is 73.4%. A rapid method for the detection of\n            <jats:italic>Coccidioides<\/jats:italic>\n            spp. directly from clinical material will greatly assist in the timely diagnosis and treatment of patients, while at the same time decreasing the risk of accidental exposure to laboratory personnel.\n          <\/jats:p>","DOI":"10.1128\/jcm.01776-06","type":"journal-article","created":{"date-parts":[[2006,11,16]],"date-time":"2006-11-16T04:22:09Z","timestamp":1163650929000},"page":"173-178","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":132,"title":["Detection of\n            <i>Coccidioides<\/i>\n            Species in Clinical Specimens by Real-Time PCR"],"prefix":"10.1128","volume":"45","author":[{"given":"M. J.","family":"Binnicker","sequence":"first","affiliation":[{"name":"Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic and Mayo Clinic College of Medicine, Rochester, Minnesota 55905"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S. P.","family":"Buckwalter","sequence":"additional","affiliation":[{"name":"Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic and Mayo Clinic College of Medicine, Rochester, Minnesota 55905"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. J.","family":"Eisberner","sequence":"additional","affiliation":[{"name":"Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic and Mayo Clinic College of Medicine, Rochester, Minnesota 55905"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. A.","family":"Stewart","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathology, Mayo Clinic Arizona, Scottsdale, Arizona 85259"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. E.","family":"McCullough","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathology, Mayo Clinic Arizona, Scottsdale, Arizona 85259"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S. L.","family":"Wohlfiel","sequence":"additional","affiliation":[{"name":"Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic and Mayo Clinic College of Medicine, Rochester, Minnesota 55905"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N. L.","family":"Wengenack","sequence":"additional","affiliation":[{"name":"Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic and Mayo Clinic College of Medicine, Rochester, Minnesota 55905"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1097\/00002030-199007000-00015"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/lt.20820"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.2.778-783.2004"},{"key":"e_1_3_2_5_2","first-page":"573","volume":"8","year":"2001","unstructured":"Bielawski, K., A. Zaczek, U. Lisowska, A. Dybikowska, A. Kowalska, and B. Falkieiewicz. 2001. The suitability of DNA extracted from formalin-fixed, paraffin-embedded tissues for double differential polymerase chain reaction analysis. Int. J. Mol. Med.8:573-578.","journal-title":"Int. J. Mol. 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