{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,17]],"date-time":"2025-10-17T13:35:33Z","timestamp":1760708133172},"reference-count":25,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2010,2,1]],"date-time":"2010-02-01T00:00:00Z","timestamp":1264982400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2010,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            A rapid two-step scheme based on PCR amplification and enzymatic digestion analysis of a 226-bp fragment of the 16S rRNA gene was developed to identify the\n            <jats:italic>Legionella<\/jats:italic>\n            genus by PCR amplification and to differentiate the\n            <jats:italic>Legionella pneumophila<\/jats:italic>\n            and non-\n            <jats:italic>Legionella pneumophila<\/jats:italic>\n            species by enzymatic digestion analysis. Among 42 ATCC strains (16 strains of\n            <jats:italic>L. pneumophila<\/jats:italic>\n            and 26 strains of non-\n            <jats:italic>L. pneumophila<\/jats:italic>\n            ) and 200\n            <jats:italic>Legionella<\/jats:italic>\n            isolates from environmental water samples, including pools, rivers, lakes, and cooling towers in Guangdong province, 99.59% of\n            <jats:italic>L. pneumophila<\/jats:italic>\n            and non-\n            <jats:italic>L. pneumophila<\/jats:italic>\n            strains were correctly identified and differentiated by this scheme. The procedure of this two-step identification and differentiation scheme is simple and takes only about 4 h. These results suggest that this two-step scheme provides a simple and convenient method for the rapid identification and differentiation of\n            <jats:italic>L. pneumophila<\/jats:italic>\n            and non-\n            <jats:italic>L. pneumophila<\/jats:italic>\n            species.\n          <\/jats:p>","DOI":"10.1128\/jcm.01778-09","type":"journal-article","created":{"date-parts":[[2009,12,10]],"date-time":"2009-12-10T02:40:46Z","timestamp":1260412846000},"page":"433-439","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":8,"title":["Two-Step Scheme for Rapid Identification and Differentiation of\n            <i>Legionella pneumophila<\/i>\n            and Non-\n            <i>Legionella pneumophila<\/i>\n            Species"],"prefix":"10.1128","volume":"48","author":[{"given":"Xiao-Yong","family":"Zhan","sequence":"first","affiliation":[{"name":"Guangzhou Kingmed Center for Clinical Laboratory, Guangzhou 510330, Guangdong, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lian-Qing","family":"Li","sequence":"additional","affiliation":[{"name":"Shanxi Center for Clinical Laboratories, Taiyuan 030012, Shanxi, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chao-Hui","family":"Hu","sequence":"additional","affiliation":[{"name":"Guangzhou Kingmed Center for Clinical Laboratory, Guangzhou 510330, Guangdong, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qing-Yi","family":"Zhu","sequence":"additional","affiliation":[{"name":"Guangzhou Kingmed Center for Clinical Laboratory, Guangzhou 510330, Guangdong, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkg500"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.5.1709-1712.2000"},{"key":"e_1_3_2_4_2","first-page":"871","volume":"23","year":"2004","unstructured":"Den Boer, J. 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