{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T16:14:40Z","timestamp":1785428080886,"version":"3.56.0"},"reference-count":38,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2026,4,8]],"date-time":"2026-04-08T00:00:00Z","timestamp":1775606400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,4,8]],"date-time":"2026-04-08T00:00:00Z","timestamp":1775606400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["P01 AI186818-01"],"award-info":[{"award-number":["P01 AI186818-01"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R01 AI063503"],"award-info":[{"award-number":["R01 AI063503"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006108","name":"National Center for Advancing Translational Sciences","doi-asserted-by":"publisher","award":["UL1TR001881-01"],"award-info":[{"award-number":["UL1TR001881-01"]}],"id":[{"id":"10.13039\/100006108","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2026,4,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      Current diagnostic approaches for mucormycosis are often limited by low sensitivity and prolonged turnaround times, which result in delayed treatment and poor clinical outcomes. We developed a novel diagnostic method utilizing a colorimetric loop-mediated isothermal amplification (LAMP) assay for the rapid and sensitive detection of mucormycosis. The assay incorporates specifically designed primers capable of detecting as low as 0.001 picograms (pg) of spiked genomic DNA from Mucorales fungi. This LAMP assay demonstrated a high sensitivity of 98% and a 100% specificity of detecting fungal ribosomal DNA (rDNA) in bronchoalveolar lavage (BAL) samples collected from mice infected with Mucorales fungi (\n                      <jats:italic toggle=\"yes\">n<\/jats:italic>\n                      = 48) or from an uninfected control group (\n                      <jats:italic toggle=\"yes\">n<\/jats:italic>\n                      = 15). To align the assay with clinical antifungal therapy, a subset of infected mice was treated with either liposomal amphotericin B (LAMB) or a combination of LAMB and a humanized monoclonal antibody (VX-01) targeting the Mucorales-specific surface protein CotH3. Consistent with the treatment efficacy, the LAMP assay detected significantly lower fungal burdens in BAL samples from mice receiving the combination therapy compared to those treated with LAMB alone or placebo. Further validation was conducted using BAL samples from patients diagnosed with mucormycosis (\n                      <jats:italic toggle=\"yes\">n<\/jats:italic>\n                      = 24) or aspergillosis (\n                      <jats:italic toggle=\"yes\">n<\/jats:italic>\n                      = 17). The assay demonstrated a sensitivity of 79% and a specificity of 94%. These findings highlight the diagnostic potential of this LAMP-based assay as a point-of-care. Its high sensitivity, specificity, and rapid turnaround time position this assay as a promising tool for early and accurate detection of mucormycosis, with the potential to improve patient management and clinical outcomes.\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>Mucormycosis is a rapidly progressive and fatal fungal infection. Timely diagnosis is critical for effective treatment, yet current diagnostic tools are slow, insensitive, or require complex laboratory procedures. In this study, we developed and validated a colorimetric loop-mediated isothermal amplification (LAMP) assay that enables rapid and reliable detection of Mucorales DNA directly from bronchoalveolar lavage (BAL) specimens. The assay demonstrated high sensitivity and specificity in both experimental mouse models and clinical samples, producing results within 1 h without the need for sophisticated equipment. This simple, robust, and cost-effective molecular diagnostic tool holds great potential for early detection of mucormycosis, facilitating prompt antifungal therapy and improving patient survival.<\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/jcm.01790-25","type":"journal-article","created":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T15:14:40Z","timestamp":1772118880000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["Rapid and reliable diagnosis of mucormycosis using colorimetric loop-mediated isothermal amplification"],"prefix":"10.1128","volume":"64","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8295-7222","authenticated-orcid":false,"given":"Yiyou","family":"Gu","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05h4zj272","id-type":"ROR","asserted-by":"publisher"}],"name":"The Lundquist Institute at Harbor-University of California Los Angeles (UCLA) Medical Center","place":["Torrance, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Belal A.","family":"Ibrahim","sequence":"additional","affiliation":[{"name":"Vitalex Biosciences LLC","place":["Trabuco Canyon, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Teclegiorgis","family":"Gebremariam","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05h4zj272","id-type":"ROR","asserted-by":"publisher"}],"name":"The Lundquist Institute at Harbor-University of California Los Angeles (UCLA) Medical Center","place":["Torrance, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sondus","family":"Alkhazraji","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05h4zj272","id-type":"ROR","asserted-by":"publisher"}],"name":"The Lundquist Institute at Harbor-University of California Los Angeles (UCLA) Medical Center","place":["Torrance, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robina","family":"Aerts","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, University Hospitals Leuven","place":["Leuven, Belgium"]},{"name":"Department of Microbiology Immunology and Transplantation, KU Leuven","place":["Leuven, Belgium"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuri","family":"Vanbiervliet","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, University Hospitals Leuven","place":["Leuven, Belgium"]},{"name":"Department of Microbiology Immunology and Transplantation, KU Leuven","place":["Leuven, Belgium"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8668-1350","authenticated-orcid":true,"given":"Katrien","family":"Lagrou","sequence":"additional","affiliation":[{"name":"Department of Microbiology Immunology and Transplantation, KU Leuven","place":["Leuven, Belgium"]},{"name":"Department of Laboratory Medicine, National Reference Center for Mycosis, University Hospitals Leuven","place":["Leuven, Belgium"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johan","family":"Maertens","sequence":"additional","affiliation":[{"name":"Department of Microbiology Immunology and Transplantation, KU Leuven","place":["Leuven, Belgium"]},{"name":"Department of Hematology, University Hospitals Leuven","place":["Leuven, Belgium"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00fcrgen","family":"Prattes","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02n0bts35","id-type":"ROR","asserted-by":"publisher"}],"name":"Division of Infectious Diseases, Department of Medicine, Translational Mycology Research Center, Excellence Centre for Medical Mycology (ECMM), Medical University of Graz","place":["Graz, Austria"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthias","family":"Egger","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02n0bts35","id-type":"ROR","asserted-by":"publisher"}],"name":"Division of Infectious Diseases, Department of Medicine, Translational Mycology Research Center, Excellence Centre for Medical Mycology (ECMM), Medical University of Graz","place":["Graz, Austria"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karl","family":"Dichtl","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02n0bts35","id-type":"ROR","asserted-by":"publisher"}],"name":"Diagnostic and Research Institute of Hygiene, Microbiology and Environmental Medicine, Medical University of Graz","place":["Graz, Austria"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey D.","family":"Jenks","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04keapj48","id-type":"ROR","asserted-by":"publisher"}],"name":"Durham County Department of Public Health","place":["Durham, USA"]},{"id":[{"id":"https:\/\/ror.org\/00py81415","id-type":"ROR","asserted-by":"publisher"}],"name":"Division of Infectious Diseases, Department of Medicine, Duke University","place":["Durham, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1653-2824","authenticated-orcid":true,"given":"Martin","family":"Hoenigl","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02n0bts35","id-type":"ROR","asserted-by":"publisher"}],"name":"Division of Infectious Diseases, Department of Medicine, Translational Mycology Research Center, Excellence Centre for Medical Mycology (ECMM), Medical University of Graz","place":["Graz, Austria"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3787-8530","authenticated-orcid":true,"given":"Ashraf S.","family":"Ibrahim","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05h4zj272","id-type":"ROR","asserted-by":"publisher"}],"name":"The Lundquist Institute at Harbor-University of California Los Angeles (UCLA) Medical Center","place":["Torrance, USA"]},{"name":"Vitalex Biosciences LLC","place":["Trabuco Canyon, USA"]},{"id":[{"id":"https:\/\/ror.org\/046rm7j60","id-type":"ROR","asserted-by":"publisher"}],"name":"David Geffen School of Medicine at UCLA","place":["Los Angeles, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.3390\/jof6040265"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a019562"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.18.3.556-569.2005"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(19)30312-3"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1086\/590004"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid2709.210934"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.4103\/ijo.IJO_310_21"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.dsx.2021.05.019"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2023.1254919"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41564-022-01172-2"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S2666-5247(24)00042-9"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.12659\/MSM.939088"},{"key":"e_1_3_4_14_2","unstructured":"Anonymous. 2022. WHO fungal priority pathogens list to guide research development and public health action. Available from: https:\/\/www.who.int\/publications\/i\/item\/9789240060241doi:https:\/\/www.who.int\/publications\/i\/item\/9789240060241"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10156-009-0669-9"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.3390\/cells10081931"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2024.106981"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.2144\/btn-2022-0037"},{"key":"e_1_3_4_19_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciz1008"},{"key":"e_1_3_4_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-374984-0.00639-2"},{"key":"e_1_3_4_21_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-018-0931-z"},{"key":"e_1_3_4_22_2","doi-asserted-by":"publisher","DOI":"10.1378\/chest.117.1.279"},{"key":"e_1_3_4_23_2","doi-asserted-by":"publisher","DOI":"10.4103\/abr.abr_297_22"},{"key":"e_1_3_4_24_2","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.ads7369"},{"key":"e_1_3_4_25_2","volume-title":"Harrison\u2019s principles of internal medicine","author":"Spellberg B","year":"2022","unstructured":"Spellberg B, Ibrahim AS. 2022. Mucormycosis. In Loscalzo J, Fauci A, Kasper D, Hauser S, Longo D, Jameson JL (ed), Harrison\u2019s principles of internal medicine, 21st ed. McGraw-Hill Education, New York, NY.","edition":"21"},{"key":"e_1_3_4_26_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cit094"},{"key":"e_1_3_4_27_2","doi-asserted-by":"publisher","DOI":"10.1111\/myc.12837"},{"key":"e_1_3_4_28_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.000375"},{"key":"e_1_3_4_29_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciab1066"},{"key":"e_1_3_4_30_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.01409-22"},{"key":"e_1_3_4_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00746-18"},{"key":"e_1_3_4_32_2","doi-asserted-by":"publisher","DOI":"10.1093\/mmy\/myae081"},{"key":"e_1_3_4_33_2","doi-asserted-by":"publisher","DOI":"10.1093\/mmy\/myae007"},{"key":"e_1_3_4_34_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkr375"},{"key":"e_1_3_4_35_2","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.aaw1327"},{"key":"e_1_3_4_36_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.01476-23"},{"key":"e_1_3_4_37_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.00215-22"},{"key":"e_1_3_4_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0001-2998(78)80014-2"},{"key":"e_1_3_4_39_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/39.4.561"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jcm.01790-25","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jcm.01790-25","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,8]],"date-time":"2026-04-08T13:01:04Z","timestamp":1775653264000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/jcm.01790-25"}},"subtitle":[],"editor":[{"given":"Kimberly E.","family":"Hanson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2026,4,8]]},"references-count":38,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2026,4,8]]}},"alternative-id":["10.1128\/jcm.01790-25"],"URL":"https:\/\/doi.org\/10.1128\/jcm.01790-25","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,4,8]]},"assertion":[{"value":"2025-12-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-12-17","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-02-26","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e01790-25"}}