{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T02:20:03Z","timestamp":1791426003881,"version":"4.3.3"},"reference-count":23,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2012,12,1]],"date-time":"2012-12-01T00:00:00Z","timestamp":1354320000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2012,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Reported methods for the detection of the yellow fever viral genome are beset by limitations in sensitivity, specificity, strain detection spectra, and suitability to laboratories with simple infrastructure in areas of endemicity. We describe the development of two different approaches affording sensitive and specific detection of the yellow fever genome: a real-time reverse transcription-quantitative PCR (RT-qPCR) and an isothermal protocol employing the same primer-probe set but based on helicase-dependent amplification technology (RT-tHDA). Both assays were evaluated using yellow fever cell culture supernatants as well as spiked and clinical samples. We demonstrate reliable detection by both assays of different strains of yellow fever virus with improved sensitivity and specificity. The RT-qPCR assay is a powerful tool for reference or diagnostic laboratories with real-time PCR capability, while the isothermal RT-tHDA assay represents a useful alternative to earlier amplification techniques for the molecular diagnosis of yellow fever by field or point-of-care laboratories.<\/jats:p>","DOI":"10.1128\/jcm.01799-12","type":"journal-article","created":{"date-parts":[[2012,10,11]],"date-time":"2012-10-11T09:02:16Z","timestamp":1349946136000},"page":"4054-4060","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":176,"title":["Advanced Yellow Fever Virus Genome Detection in Point-of-Care Facilities and Reference Laboratories"],"prefix":"10.1128","volume":"50","author":[{"given":"Cristina","family":"Domingo","sequence":"first","affiliation":[{"name":"Robert Koch Institute, Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pranav","family":"Patel","sequence":"additional","affiliation":[{"name":"Robert Koch Institute, Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jasmin","family":"Yillah","sequence":"additional","affiliation":[{"name":"Robert Koch Institute, Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manfred","family":"Weidmann","sequence":"additional","affiliation":[{"name":"University Medical Center, Department of Virology, G\u00f6ttingen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jairo A.","family":"M\u00e9ndez","sequence":"additional","affiliation":[{"name":"Instituto Nacional de Salud, Laboratorio de Virolog\u00eda, Bogot\u00e1, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emmanuel Rivalyn","family":"Nakoun\u00e9","sequence":"additional","affiliation":[{"name":"Institute Pasteur de Bangui, Bangui, Central African Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthias","family":"Niedrig","sequence":"additional","affiliation":[{"name":"Robert Koch Institute, Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","first-page":"1349","article-title":"Dengue virus infection in Africa","volume":"17","author":"Amarasinghe A","year":"2011","unstructured":"AmarasingheA KuritskJN LetsonGW MargolisHS. 2011. 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A clinico-pathological study of yellow fever in Nigeria. W. Afr. Med. J. 6:137\u2013146.","journal-title":"W. Afr. Med. J."},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2011.02.025"},{"key":"e_1_3_3_17_2","unstructured":"Pan American Health Organization (PAHO). 2005. Control of yellow fever: field guide. PAHO scientific and technical publication no. 603. Pan American Health Organization Washington DC. http:\/\/www.paho.org\/english\/ad\/fch\/im\/fieldguide_yellowfever.pdf."},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-008-2287-2"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msm092"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(99)09233-8"},{"key":"e_1_3_3_21_2","volume-title":"Yellow fever.","author":"Vainio J","year":"1998","unstructured":"VainioJ CuttsF. 1998. Yellow fever. 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Rec."}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.01799-12","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.01799-12","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T06:07:07Z","timestamp":1646460427000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.01799-12"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,12]]},"references-count":23,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2012,12]]}},"alternative-id":["10.1128\/JCM.01799-12"],"URL":"https:\/\/doi.org\/10.1128\/jcm.01799-12","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,12]]},"assertion":[{"value":"2012-07-10","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2012-10-01","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2012-11-16","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}