{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T02:38:56Z","timestamp":1773283136332,"version":"3.50.1"},"reference-count":16,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2008,5,1]],"date-time":"2008-05-01T00:00:00Z","timestamp":1209600000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2008,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            In\n            <jats:italic>Neisseria gonorrhoeae<\/jats:italic>\n            , the mosaic structure of the\n            <jats:italic>penA<\/jats:italic>\n            gene (encoding penicillin-binding protein 2 [PBP 2]), which is composed of fragments of the\n            <jats:italic>penA<\/jats:italic>\n            genes from\n            <jats:italic>Neisseria cinerea<\/jats:italic>\n            and\n            <jats:italic>Neisseria perflava<\/jats:italic>\n            , has been significantly associated with decreased susceptibility to cephalosporins, particularly oral cephalosporins. The aim of this study was to develop a rapid assay for the detection of mosaic PBP 2 of\n            <jats:italic>N. gonorrhoeae<\/jats:italic>\n            by real-time PCR. This assay successfully detected the mosaic\n            <jats:italic>penA<\/jats:italic>\n            gene of\n            <jats:italic>N. gonorrhoeae<\/jats:italic>\n            , and its sensitivity was \u226510\n            <jats:sup>1<\/jats:sup>\n            copies\/reaction. Six hundred twenty-one clinical strains were examined by this assay for the presence of mosaic PBP 2, which was detected in 85 (39.4%) of 216 strains from 2002, 69 (40.6%) of 170 strains from 2003, 71 (44.4%) of 160 strains from 2004, and 31 (41.3%) of 75 strains from 2005. The MICs of cephalosporins for strains with the mosaic PBP 2 detected by the assay were statistically higher than those for strains without the mosaic PBP 2. One hundred sixty-six (64.8%) of 256 strains with the mosaic PBP 2 exhibited cefixime MICs of \u22650.5 \u03bcg\/ml. The emergence and spread of strains with mosaic PBP 2 could be a threat to the cefixime treatment of gonorrhea. This real-time PCR assay for the detection of mosaic PBP 2 of\n            <jats:italic>N. gonorrhoeae<\/jats:italic>\n            is thus useful in the prediction of decreased susceptibilities to oral cephalosporins.\n          <\/jats:p>","DOI":"10.1128\/jcm.01800-07","type":"journal-article","created":{"date-parts":[[2008,3,27]],"date-time":"2008-03-27T01:00:42Z","timestamp":1206579642000},"page":"1804-1810","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":41,"title":["Rapid Detection of the Mosaic Structure of the\n            <i>Neisseria gonorrhoeae penA<\/i>\n            Gene, Which Is Associated with Decreased Susceptibilities to Oral Cephalosporins"],"prefix":"10.1128","volume":"46","author":[{"given":"Susumu","family":"Ochiai","sequence":"first","affiliation":[{"name":"Advanced Medicine Development Department, R&amp;D and Business Development Segment, Mitsubishi Chemical Medience Corporation, Tokyo 174-8555, Japan"},{"name":"Department of Urology, School of Medicine, Gifu University, Gifu 501-1194, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroaki","family":"Ishiko","sequence":"additional","affiliation":[{"name":"R&amp;D and Business Development Segment, Mitsubishi Chemical Medience Corporation, Tokyo 174-8555, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mitsuru","family":"Yasuda","sequence":"additional","affiliation":[{"name":"Department of Urology, School of Medicine, Gifu University, Gifu 501-1194, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takashi","family":"Deguchi","sequence":"additional","affiliation":[{"name":"Department of Urology, School of Medicine, Gifu University, Gifu 501-1194, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/s101560170034"},{"key":"e_1_3_1_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1990.tb00664.x"},{"key":"e_1_3_1_4_2","unstructured":"Performance standards for antimicrobial susceptibility testing; 16th informational supplement. 2006"},{"key":"e_1_3_1_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.30.6.856"},{"key":"e_1_3_1_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.42.11.2799"},{"key":"e_1_3_1_7_2","doi-asserted-by":"publisher","DOI":"10.1099\/13500872-141-3-611"},{"key":"e_1_3_1_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.48.8.3185-3187.2004"},{"key":"e_1_3_1_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.49.1.137-143.2005"},{"key":"e_1_3_1_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01604-06"},{"key":"e_1_3_1_11_2","unstructured":"Molecular cloning: a laboratory manual 1989"},{"key":"e_1_3_1_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.45.12.3603-3606.2001"},{"key":"e_1_3_1_13_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkm166"},{"key":"e_1_3_1_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.46.3.769-777.2002"},{"key":"e_1_3_1_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00626-06"},{"key":"e_1_3_1_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01307-06"},{"key":"e_1_3_1_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00306-07"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.01800-07","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.01800-07","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T06:23:03Z","timestamp":1646461383000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.01800-07"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,5]]},"references-count":16,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2008,5]]}},"alternative-id":["10.1128\/JCM.01800-07"],"URL":"https:\/\/doi.org\/10.1128\/jcm.01800-07","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,5]]},"assertion":[{"value":"2007-09-10","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2008-03-11","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2008-05-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}