{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T02:00:50Z","timestamp":1791252050996,"version":"4.1.0"},"reference-count":25,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2015,11,1]],"date-time":"2015-11-01T00:00:00Z","timestamp":1446336000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2015,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The goal of this retrospective study was to evaluate the performance of different diagnostic tests for Legionnaires' disease in a clinical setting where\n            <jats:named-content content-type=\"genus-species\">Legionella pneumophila<\/jats:named-content>\n            PCR had been introduced. Electronic medical records at the Cleveland Clinic were searched for\n            <jats:named-content content-type=\"genus-species\">Legionella<\/jats:named-content>\n            urinary antigen (UAG), culture, and PCR tests ordered from March 2010 through December 2013. For cases where two or more test methods were performed and at least one was positive, the medical record was reviewed for relevant clinical and epidemiologic factors. Excluding repeat testing on a given patient, 19,912 tests were ordered (12,569 UAG, 3,747 cultures, and 3,596 PCR) with 378 positive results. The positivity rate for each method was 0.4% for culture, 0.8% for PCR, and 2.7% for UAG. For 37 patients, at least two test methods were performed with at least one positive result: 10 (27%) cases were positive by all three methods, 16 (43%) were positive by two methods, and 11 (30%) were positive by one method only. For the 32 patients with medical records available, clinical presentation was consistent with proven or probable\n            <jats:named-content content-type=\"genus-species\">Legionella<\/jats:named-content>\n            infection in 84% of the cases. For those cases, the sensitivities of culture, PCR, and UAG were 50%, 92%, and 96%, respectively. The specificities were 100% for culture and 99.9% for PCR and UAG.\n          <\/jats:p>","DOI":"10.1128\/jcm.01808-15","type":"journal-article","created":{"date-parts":[[2015,9,2]],"date-time":"2015-09-02T22:33:11Z","timestamp":1441233191000},"page":"3474-3477","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":27,"title":["Utility of PCR, Culture, and Antigen Detection Methods for Diagnosis of Legionellosis"],"prefix":"10.1128","volume":"53","author":[{"given":"Derrick J.","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine, Cleveland Clinic, Cleveland, Ohio, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gary W.","family":"Procop","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Cleveland Clinic, Cleveland, Ohio, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sherilynn","family":"Vogel","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Cleveland Clinic, Cleveland, Ohio, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Belinda","family":"Yen-Lieberman","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Cleveland Clinic, Cleveland, Ohio, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandra S.","family":"Richter","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Cleveland Clinic, Cleveland, Ohio, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/9781555817381.ch49"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00029-14"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1101\/gr.7229808"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng1447"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00052-09"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1086\/341087"},{"key":"e_1_3_3_8_2","volume-title":"Legionella (Legionnaires Disease and Pontiac fever)","author":"Centers for Disease Control and Prevention","year":"2013","unstructured":"Centers for Disease Control and Prevention. 2013. Legionella (Legionnaires Disease and Pontiac fever). Centers for Disease Control and Prevention, Atlanta, GA. http:\/\/www.cdc.gov\/legionella\/clinicians.html. Accessed 29 June 2015."},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1086\/511159"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1086\/342903"},{"key":"e_1_3_3_11_2","first-page":"1083","article-title":"Legionellosis\u2014United States, 2000\u20132009","volume":"60","author":"Hicks L","year":"2011","unstructured":"Hicks L, Garrison L, Nelson GE, Hampton LM. 2011. Legionellosis\u2014United States, 2000\u20132009. MMWR Morb Mortal Wkly Rep 60:1083\u20131086.","journal-title":"MMWR Morb Mortal Wkly Rep"},{"key":"e_1_3_3_12_2","volume-title":"Abstr IDWeek 2014","author":"Chen D","year":"2014","unstructured":"Chen D, Procop G, Richter SS. 2014. Evaluation of Legionella diagnostic testing by urinary antigen, culture, and PCR, poster 1408. Abstr IDWeek 2014, IDWeek, Arlington, VA."},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.7.3327-3330.2003"},{"key":"e_1_3_3_14_2","first-page":"85","article-title":"Clinical utility of a Legionella pneumophila urinary antigen test in a large university teaching hospital","volume":"14","author":"Dionne M","year":"2003","unstructured":"Dionne M, Hatchette T, Forward K. 2003. Clinical utility of a Legionella pneumophila urinary antigen test in a large university teaching hospital. Can J Infect Dis 14:85\u201388.","journal-title":"Can J Infect Dis"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1378\/chest.08-2602"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.2.838-840.2003"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1111\/1469-0691.12311"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00176-10"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(14)70713-3"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.5.1709-1712.2000"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.7.2618-2626.2001"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01196-07"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.20.4.605-607.1984"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1080\/00365540600561777"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cit504"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.7.2974-2979.2003"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.01808-15","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.01808-15","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T08:44:52Z","timestamp":1646469892000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.01808-15"}},"subtitle":[],"editor":[{"given":"A. B.","family":"Onderdonk","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2015,11]]},"references-count":25,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2015,11]]}},"alternative-id":["10.1128\/JCM.01808-15"],"URL":"https:\/\/doi.org\/10.1128\/jcm.01808-15","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,11]]},"assertion":[{"value":"2015-07-05","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-08-13","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-10-16","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}