{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T16:06:31Z","timestamp":1792598791339,"version":"4.5.0"},"reference-count":29,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2011,4,1]],"date-time":"2011-04-01T00:00:00Z","timestamp":1301616000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2011,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The emergence of\n                    <jats:named-content content-type=\"genus-species\">Scedosporium<\/jats:named-content>\n                    infections in diverse groups of individuals, which are often treatment refractory, warrants timely and accurate laboratory diagnosis. Species- or group-specific primers based on internal transcribed spacer (ITS) sequence polymorphisms were designed for\n                    <jats:named-content content-type=\"genus-species\">Scedosporium aurantiacum<\/jats:named-content>\n                    ,\n                    <jats:named-content content-type=\"genus-species\">Scedosporium dehoogii<\/jats:named-content>\n                    ,\n                    <jats:named-content content-type=\"genus-species\">Scedosporium prolificans<\/jats:named-content>\n                    ,\n                    <jats:named-content content-type=\"genus-species\">Pseudallescheria boydii<\/jats:named-content>\n                    species complex (former clade 5)\n                    <jats:italic>\/Pseudallescheria apiosperma<\/jats:italic>\n                    (formerly classified as\n                    <jats:named-content content-type=\"genus-species\">S. apiospermum<\/jats:named-content>\n                    sensu lato) and\n                    <jats:named-content content-type=\"genus-species\">Pseudallescheria minutispora<\/jats:named-content>\n                    . Primers for\n                    <jats:named-content content-type=\"genus-species\">S. aurantiacum<\/jats:named-content>\n                    ,\n                    <jats:named-content content-type=\"genus-species\">S. prolificans<\/jats:named-content>\n                    , and\n                    <jats:named-content content-type=\"genus-species\">P. boydii<\/jats:named-content>\n                    species complex\/\n                    <jats:named-content content-type=\"genus-species\">P. apiosperma<\/jats:named-content>\n                    were incorporated into a multiplex PCR assay for the detection and identification of the three major clinically important\n                    <jats:named-content content-type=\"genus-species\">Scedosporium<\/jats:named-content>\n                    species and validated using sputum specimens collected from patients seen at a major Australian cystic fibrosis clinic. The multiplex PCR assay showed 100% specificity in identifying the three major clinically relevant\n                    <jats:named-content content-type=\"genus-species\">Scedosporium<\/jats:named-content>\n                    species from pure culture. When evaluated using DNA extracts from sputa, sensitivity and specificity of the multiplex PCR assay were 62.1% and 97.2%, respectively. This highly species-specific multiplex PCR assay offers a rapid and simple method of detection of the most clinically important\n                    <jats:named-content content-type=\"genus-species\">Scedosporium<\/jats:named-content>\n                    species in respiratory tract specimens.\n                  <\/jats:p>","DOI":"10.1128\/jcm.01810-10","type":"journal-article","created":{"date-parts":[[2011,2,16]],"date-time":"2011-02-16T22:37:05Z","timestamp":1297895825000},"page":"1508-1512","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":45,"title":["Development and Validation of a Multiplex PCR for Detection of Scedosporium spp. in Respiratory Tract Specimens from Patients with Cystic Fibrosis"],"prefix":"10.1128","volume":"49","author":[{"given":"Azian","family":"Harun","sequence":"first","affiliation":[{"name":"Molecular Mycology Research Laboratory, Sydney Medical School\u2014Westmead Hospital, Centre for Infectious Diseases and Microbiology, Westmead Millennium Institute, Sydney Emerging Infections and Biosecurity Institute, The University of Sydney, Sydney, NSW, Australia"},{"name":"School of Medical Sciences, Universiti Sains Malaysia, Penang, Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher C.","family":"Blyth","sequence":"additional","affiliation":[{"name":"Molecular Mycology Research Laboratory, Sydney Medical School\u2014Westmead Hospital, Centre for Infectious Diseases and Microbiology, Westmead Millennium Institute, Sydney Emerging Infections and Biosecurity Institute, The University of Sydney, Sydney, NSW, Australia"},{"name":"School of Paediatrics and Child Health, University of Western Australia, Princess Margaret Hospital, Perth, WA, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Felix","family":"Gilgado","sequence":"additional","affiliation":[{"name":"Molecular Mycology Research Laboratory, Sydney Medical 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