{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T05:12:27Z","timestamp":1790745147211,"version":"4.1.0"},"reference-count":16,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2014,11,1]],"date-time":"2014-11-01T00:00:00Z","timestamp":1414800000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2014,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Sepsis caused by\n            <jats:named-content content-type=\"genus-species\">Staphylococcus aureus<\/jats:named-content>\n            is a major health problem worldwide. Better outcomes are achieved when rapid diagnosis and determination of methicillin susceptibility enable early optimization of antimicrobial therapy. Eight large clinical laboratories, seven from the United States and one from Scotland, evaluated the combination of the\n            <jats:named-content content-type=\"genus-species\">Staphylococcus<\/jats:named-content>\n            <jats:italic>Quick<\/jats:italic>\n            FISH BC and the new\n            <jats:italic>mecA Xpress<\/jats:italic>\n            FISH assay (both AdvanDx, Woburn, MA, USA) for the detection of methicillin-resistant\n            <jats:named-content content-type=\"genus-species\">S. aureus<\/jats:named-content>\n            in positive blood cultures. Blood cultures flagged as positive by automated blood culture instruments and demonstrating only Gram-positive cocci in clusters on Gram stain were tested by\n            <jats:italic>Quick<\/jats:italic>\n            FISH, a 20-min assay. If only\n            <jats:named-content content-type=\"genus-species\">S. aureus<\/jats:named-content>\n            was detected,\n            <jats:italic>mecA Xpress<\/jats:italic>\n            FISH testing followed. The recovered\n            <jats:named-content content-type=\"genus-species\">S. aureus<\/jats:named-content>\n            isolates were tested by cefoxitin disk diffusion as the reference method. The\n            <jats:italic>Quick<\/jats:italic>\n            FISH assay results were concordant with the routine phenotypic testing methods of the testing laboratories in 1,211\/1,221 (99.1%) samples and detected 488\/491\n            <jats:named-content content-type=\"genus-species\">S. aureus<\/jats:named-content>\n            organisms (sensitivity, 99.4%; specificity, 99.6%). Approximately 60% of the samples (730) contained coagulase-negative staphylococci or nonstaphylococci as assessed by the\n            <jats:italic>Quick<\/jats:italic>\n            FISH assay and were not tested further. The 458 compliant samples positive exclusively for\n            <jats:named-content content-type=\"genus-species\">S. aureus<\/jats:named-content>\n            by the\n            <jats:italic>Quick<\/jats:italic>\n            FISH assay were tested by the\n            <jats:italic>mecA Xpress<\/jats:italic>\n            FISH assay, which detected 209 of 211 methicillin-resistant\n            <jats:named-content content-type=\"genus-species\">S. aureus<\/jats:named-content>\n            organisms (sensitivity, 99.1%; specificity, 99.6%). The\n            <jats:italic>mecA Xpress<\/jats:italic>\n            FISH assay also showed high reproducibility, with 534\/540 tests performed by 6 operators over 5 days achieving reproducible results (98.9% agreement). The combination of the\n            <jats:named-content content-type=\"genus-species\">Staphylococcus<\/jats:named-content>\n            <jats:italic>Quick<\/jats:italic>\n            FISH BC and\n            <jats:italic>mecA Xpress<\/jats:italic>\n            FISH assays is sensitive, specific, and reproducible for the detection of methicillin-resistant\n            <jats:named-content content-type=\"genus-species\">S. aureus<\/jats:named-content>\n            and yields complete results in 2 h after the blood culture turns positive.\n          <\/jats:p>","DOI":"10.1128\/jcm.01811-14","type":"journal-article","created":{"date-parts":[[2014,8,28]],"date-time":"2014-08-28T01:58:10Z","timestamp":1409191090000},"page":"3928-3932","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":25,"title":["An International, Prospective, Multicenter Evaluation of the Combination of AdvanDx Staphylococcus\n            <i>Quick<\/i>\n            FISH BC with\n            <i>mecA Xpress<\/i>\n            FISH for Detection of Methicillin-Resistant Staphylococcus aureus Isolates from Positive Blood Cultures"],"prefix":"10.1128","volume":"52","author":[{"given":"H.","family":"Salimnia","sequence":"first","affiliation":[{"name":"Detroit Medical Center University Laboratories, Detroit, Michigan, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. R.","family":"Fairfax","sequence":"additional","affiliation":[{"name":"Detroit Medical Center University Laboratories, Detroit, Michigan, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Lephart","sequence":"additional","affiliation":[{"name":"Detroit Medical Center University Laboratories, Detroit, Michigan, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Morgan","sequence":"additional","affiliation":[{"name":"Cedars Sinai Medical Center, Los Angeles, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. J.","family":"Gilbreath","sequence":"additional","affiliation":[{"name":"University of Washington, Seattle, Washington, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S. M.","family":"Butler-Wu","sequence":"additional","affiliation":[{"name":"University of Washington, Seattle, Washington, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K. E.","family":"Templeton","sequence":"additional","affiliation":[{"name":"Royal Infirmary, Edinburgh, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F. J.","family":"Hamilton","sequence":"additional","affiliation":[{"name":"Royal Infirmary, Edinburgh, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.","family":"Wu","sequence":"additional","affiliation":[{"name":"Columbia University Medical Center, New York, New York, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Buckner","sequence":"additional","affiliation":[{"name":"Indiana University School of Medicine, Indianapolis, Indiana, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Fuller","sequence":"additional","affiliation":[{"name":"Indiana University School of Medicine, Indianapolis, Indiana, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T. E.","family":"Davis","sequence":"additional","affiliation":[{"name":"Indiana University School of Medicine, Indianapolis, Indiana, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. M.","family":"Abdelhamed","sequence":"additional","affiliation":[{"name":"Case Western Reserve University and University Hospitals Case Medical Center, Cleveland, Ohio, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. R.","family":"Jacobs","sequence":"additional","affiliation":[{"name":"Case Western Reserve University and University Hospitals Case Medical Center, Cleveland, Ohio, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Miller","sequence":"additional","affiliation":[{"name":"Johns Hopkins University School of Medicine, Baltimore, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.","family":"Pfrommer","sequence":"additional","affiliation":[{"name":"Johns Hopkins University School of Medicine, Baltimore, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K. C.","family":"Carroll","sequence":"additional","affiliation":[{"name":"Johns Hopkins University School of Medicine, Baltimore, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1186\/1476-0711-5-2"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.05022-11"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid1806.120182"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-006-0096-0"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.290.14.1899"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1378\/chest.09-0087"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkq373"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1586\/14737159.7.3.231"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00225-12"},{"key":"e_1_3_2_11_2","volume-title":"Staphylococcus QuickFISH BC Staphylococcus aureus\/coagulase-negative staphylococci culture identification kit package insert","author":"AdvanDx, Inc","unstructured":"AdvanDx, Inc. Staphylococcus QuickFISH BC Staphylococcus aureus\/coagulase-negative staphylococci culture identification kit package insert. AdvanDx, Inc., Woburn, MA. http:\/\/www.advandx.com\/AdvanDX\/media\/Documents\/Package%20Inserts\/PN1878D---Staphylococcus-QuickFISH-BC-Kit.pdf."},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.171.5.2882-2885.1989"},{"key":"e_1_3_2_13_2","volume-title":"Performance standards for antimicrobial susceptibility testing; 23rd informational supplement","author":"Clinical and Laboratory Standards Institute","year":"2013","unstructured":"Clinical and Laboratory Standards Institute. 2013. Performance standards for antimicrobial susceptibility testing; 23rd informational supplement. CLSI document M100-S23. Clinical and Laboratory Standards Institute, Wayne, PA."},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/33.1.7"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1093\/biomet\/26.4.404"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02103-12"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02461-10"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.01811-14","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.01811-14","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T11:38:47Z","timestamp":1646480327000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.01811-14"}},"subtitle":[],"editor":[{"given":"B. A.","family":"Forbes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2014,11]]},"references-count":16,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2014,11]]}},"alternative-id":["10.1128\/JCM.01811-14"],"URL":"https:\/\/doi.org\/10.1128\/jcm.01811-14","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,11]]},"assertion":[{"value":"2014-06-27","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-08-21","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-10-16","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}