{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T04:33:20Z","timestamp":1790742800540,"version":"4.1.0"},"reference-count":39,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2011,3,1]],"date-time":"2011-03-01T00:00:00Z","timestamp":1298937600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2011,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The major clinically relevant species in\n            <jats:named-content content-type=\"genus-species\">Scedosporium<\/jats:named-content>\n            (teleomorph\n            <jats:named-content content-type=\"genus-species\">Pseudallescheria<\/jats:named-content>\n            ) are\n            <jats:named-content content-type=\"genus-species\">Pseudallescheria boydii<\/jats:named-content>\n            ,\n            <jats:named-content content-type=\"genus-species\">Scedosporium aurantiacum<\/jats:named-content>\n            ,\n            <jats:named-content content-type=\"genus-species\">Scedosporium apiospermum<\/jats:named-content>\n            , and\n            <jats:named-content content-type=\"genus-species\">Scedosporium prolificans<\/jats:named-content>\n            , while\n            <jats:named-content content-type=\"genus-species\">Pseudallescheria minutispora<\/jats:named-content>\n            ,\n            <jats:named-content content-type=\"genus-species\">Petriellopsis desertorum<\/jats:named-content>\n            , and\n            <jats:named-content content-type=\"genus-species\">Scedosporium dehoogii<\/jats:named-content>\n            are exceptional agents of disease. Three molecular methods targeting the partial \u03b2-tubulin gene were developed and evaluated to identify six closely related species of the\n            <jats:named-content content-type=\"genus-species\">S. apiospermum<\/jats:named-content>\n            complex using quantitative real-time PCR (qPCR), PCR-based reverse line blot (PCR-RLB), and loop-mediated isothermal amplification (LAMP). qPCR was not specific enough for the identification of all species but had the highest sensitivity. The PCR-RLB assay was efficient for the identification of five species. LAMP distinguished all six species unambiguously. The analytical sensitivities of qPCR, PCR-RLB, and LAMP combined with MagNAPure, CTAB (cetyltrimethylammonium bromide), and FTA filter (Whatman) extraction were 50, 5 \u00d7 10\n            <jats:sup>3<\/jats:sup>\n            , and 5 \u00d7 10\n            <jats:sup>2<\/jats:sup>\n            cells\/\u03bcl, respectively. When LAMP was combined with a simplified DNA extraction method using an FTA filter, identification to the species level was achieved within 2 h, including DNA extraction. The FTA-LAMP assay is therefore recommended as a cost-effective, simple, and rapid method for the identification of\n            <jats:named-content content-type=\"genus-species\">Scedosporium<\/jats:named-content>\n            species.\n          <\/jats:p>","DOI":"10.1128\/jcm.01813-10","type":"journal-article","created":{"date-parts":[[2010,12,23]],"date-time":"2010-12-23T03:29:27Z","timestamp":1293074967000},"page":"960-967","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":58,"title":["Identification of Pseudallescheria and Scedosporium Species by Three Molecular Methods"],"prefix":"10.1128","volume":"49","author":[{"given":"Qiaoyun","family":"Lu","sequence":"first","affiliation":[{"name":"Department of Dermatology, Peking University First Hospital, and Research Center for Medical Mycology, Peking University, Beijing, China"},{"name":"Centraalbureau voor Schimmelcultures KNAW Fungal Biodiversity Centre, Utrecht, Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. 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