{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T15:29:25Z","timestamp":1790004565951,"version":"4.0.1"},"reference-count":32,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2014,12,1]],"date-time":"2014-12-01T00:00:00Z","timestamp":1417392000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2014,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Rapid and effective diagnosis of brucellosis is a challenge for clinicians. Even when diagnosis is on time and therapy is initiated, meticulous follow-up appointments are crucial for ensuring the efficacy of the treatment. Due to shortcomings of serological methods, molecular diagnosis, especially real-time PCR, is becoming a main approach in laboratory diagnostics. Thus, the development of efficient procedures and standardization of the PCR tests will have a great impact on the precise detection and quantification of bacterial DNA loads, which is valuable for the medical management of brucellosis patients. We developed a new TaqMan real-time PCR directed to<jats:italic>bcsp31<\/jats:italic>, a shared gene of the brucellae. The<jats:italic>bcsp31<\/jats:italic>gene fragment was cloned into pJET1.2. Recombinant pJET1.2-<jats:italic>bcsp31<\/jats:italic>was linearized by HindIII digestion, and the product was used for the preparation of a standard curve. A panel of<jats:named-content content-type=\"genus-species\">Brucella<\/jats:named-content>spp. and non-<jats:named-content content-type=\"genus-species\">Brucella<\/jats:named-content>pathogens was tested. No bacterial genomes other than those of the brucellae were detected. According to the results, specificity of the method was 100%. In a clinical assessment, the positive-control group comprised 37 patients with microbiologically confirmed brucellosis, and 25 healthy individuals served as the negative-control group. By the end of the treatment period, there was a significant decrease in the DNA load of the 37 brucellosis patients, which persisted for the 4 weeks of monitoring after treatment, suggesting that our proposed method is an efficient monitoring tool. Serum samples prior to any treatment were collected from the 25 serologically suspicious patients and assessed by our method; 72% of these patients tested positive for brucellosis.<\/jats:p>","DOI":"10.1128\/jcm.01819-14","type":"journal-article","created":{"date-parts":[[2014,10,2]],"date-time":"2014-10-02T08:32:01Z","timestamp":1412238721000},"page":"4239-4243","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":26,"title":["Efficient Diagnosis and Treatment Follow-Up of Human Brucellosis by a Novel Quantitative TaqMan Real-Time PCR Assay: a Human Clinical Survey"],"prefix":"10.1128","volume":"52","author":[{"given":"Majid","family":"Sohrabi","sequence":"first","affiliation":[{"name":"Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ashraf","family":"Mohabati Mobarez","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nima","family":"Khoramabadi","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reza","family":"Hosseini Doust","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Islamic Azad University, Pharmaceutical Sciences Branch, Tehran, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mehrdad","family":"Behmanesh","sequence":"additional","affiliation":[{"name":"Department of Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra050570"},{"key":"e_1_3_1_3_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/21.2.283"},{"key":"e_1_3_1_4_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-6-13"},{"key":"e_1_3_1_5_2","doi-asserted-by":"publisher","DOI":"10.1186\/1746-6148-5-22"},{"key":"e_1_3_1_6_2","doi-asserted-by":"publisher","DOI":"10.1086\/588482"},{"key":"e_1_3_1_7_2","doi-asserted-by":"publisher","DOI":"10.1086\/426818"},{"key":"e_1_3_1_8_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.47160-0"},{"key":"e_1_3_1_9_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/14.1.131"},{"key":"e_1_3_1_10_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/13.3.359"},{"key":"e_1_3_1_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2007.04.002"},{"key":"e_1_3_1_12_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-005-0064-0"},{"key":"e_1_3_1_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2008.02095.x"},{"key":"e_1_3_1_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.4.1661-1664.2001"},{"key":"e_1_3_1_15_2","doi-asserted-by":"publisher","DOI":"10.2174\/1574891X11308010007"},{"key":"e_1_3_1_16_2","doi-asserted-by":"publisher","DOI":"10.1159\/000331588"},{"key":"e_1_3_1_17_2","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1501\/Vetfak_0000002368","article-title":"Investigation of Brucella antibodies in bovine sera by rose bengal plate test (RBPT), serum agglutination test (SAT), microagglutination test (MAT) and 2-mercaptoethanol-microagglutination (2-ME-MAT) test","volume":"57","author":"Sareyy\u00fcpo\u01e7lu B","year":"2010","unstructured":"Sareyy\u00fcpo\u01e7luB Cantekin M\u00fc\u015ftakZ KaanH. 2010. 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