{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T00:06:34Z","timestamp":1788998794399,"version":"build-2803163510"},"reference-count":37,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T00:00:00Z","timestamp":1747180800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T00:00:00Z","timestamp":1747180800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2025,5,14]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:sec>\n            <jats:title\/>\n            <jats:p>Accurate and rapid antimicrobial susceptibility test (AST) results from positive blood cultures are crucial for patient care and combatting antimicrobial resistance. Although recent advancements in rapid direct-from-positive blood culture (PBC) identification platforms have enabled the provision of species-level identification and some resistance marker information within hours after blood culture positivity, AST results required for clinical decision-making often require 48 h after blood culture positivity. This study evaluated the Selux next-generation phenotyping system, including an automated PBC Separator and the Selux AST system in a multicenter clinical trial for their ability to perform AST directly from PBCs for gram-negative bacilli. The PBC separator produces McFarland equivalent inocula from positive blood cultures within 1 h, facilitating direct processing on the Selux AST system. The study evaluated 162 fresh clinical PBC samples, 307 seeded clinical samples, and 87 seeded challenge samples across 4 sites for each of the 17 antimicrobials included in the panel. The results demonstrate that the Selux system\u2019s clinical performance, reproducibility, and analytical performances are consistent when using positive blood cultures held for up to 16 h after positivity on the BACTEC and BacT\/ALERT 3D and BacT\/ALERT VIRTUO blood culture systems, including all major BACTEC and BacT\/ALERT blood culture bottle types. These findings suggest that the PBC Separator with the Selux AST system is a valuable addition to the arsenal of tools available for rapid sepsis diagnosis and management.<\/jats:p>\n            <jats:sec>\n              <jats:title>IMPORTANCE<\/jats:title>\n              <jats:p>Technologies that consistently and substantially shorten the time between blood bottle positivity, organism identification, and complete AST results are crucial for ensuring that antimicrobial therapy can be tailored. The Selux PBC Separator and the Selux AST system perform rapid AST directly from positive blood culture bottles. This substantially shortens the gap between obtaining a positive blood bottle and organism identification and the availability of a fully actionable AST result.<\/jats:p>\n            <\/jats:sec>\n          <\/jats:sec>","DOI":"10.1128\/jcm.01819-24","type":"journal-article","created":{"date-parts":[[2025,4,14]],"date-time":"2025-04-14T16:16:21Z","timestamp":1744647381000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":7,"title":["Multi-center evaluation of the Selux next-generation phenotyping system for gram-negative direct-from-positive blood culture antimicrobial susceptibility testing"],"prefix":"10.1128","volume":"63","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-6306-7517","authenticated-orcid":true,"given":"Vincent","family":"Streva","sequence":"first","affiliation":[{"name":"Northwell Heath","place":["New York, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph","family":"Gajewski","sequence":"additional","affiliation":[{"name":"Element","place":["Acton, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacqueline","family":"Pento","sequence":"additional","affiliation":[{"name":"Northwell Heath","place":["New York, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-6144-1533","authenticated-orcid":false,"given":"Alamelu","family":"Chandrasekaran","sequence":"additional","affiliation":[{"name":"The Center for Advanced Laboratory Medicine, Department of Pathology and Cell Biology, Columbia University Irving Medical Center","place":["New York, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matt","family":"Green","sequence":"additional","affiliation":[{"name":"Element","place":["Acton, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jill","family":"Lindley","sequence":"additional","affiliation":[{"name":"Element Iowa City (JMI Laboratories)","place":["North Liberty, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Micahel","family":"Huband","sequence":"additional","affiliation":[{"name":"Element Iowa City (JMI Laboratories)","place":["North Liberty, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Asmae","family":"El ganbour","sequence":"additional","affiliation":[{"name":"Selux Diagnostics","place":["Charlestown, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristen","family":"Roberts","sequence":"additional","affiliation":[{"name":"Selux Diagnostics","place":["Charlestown, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kelly","family":"Flentie","sequence":"additional","affiliation":[{"name":"Selux Diagnostics","place":["Charlestown, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingzi","family":"Sherman","sequence":"additional","affiliation":[{"name":"Selux Diagnostics","place":["Charlestown, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9039-3693","authenticated-orcid":true,"given":"Eric","family":"Stern","sequence":"additional","affiliation":[{"name":"Selux Diagnostics","place":["Charlestown, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0767-6826","authenticated-orcid":true,"given":"Gregory J.","family":"Berry","sequence":"additional","affiliation":[{"name":"The Center for Advanced Laboratory Medicine, Department of Pathology and Cell Biology, Columbia University Irving Medical Center","place":["New York, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1183\/13993003.00057-2019"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1001\/jamanetworkopen.2022.49353"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-022-07142-2"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1177\/2042098614554919"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.4081\/idr.2017.6839"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-020-0327-x"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics11040427"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1086\/647952"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00803-11"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.02364-20"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.33552\/ASOAJ.2020.02.000530"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.00546-23"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-35916-0"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciw201"},{"key":"e_1_3_3_16_2","unstructured":"U.S. Food and Drug Administration. 2024. Antibacterial susceptibility test interpretive criteria. Available from: https:\/\/www.fda.gov\/drugs\/development-resources\/antibacterial-susceptibility-test-interpretive-criteria. Retrieved 17 Mar 2025."},{"key":"e_1_3_3_17_2","volume-title":"M100 performance standards for antimicrobial susceptibility testing","author":"CLSI","year":"2024","unstructured":"CLSI. 2024. M100 performance standards for antimicrobial susceptibility testing. Clinical and Laboratory Standards Institute."},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1093\/ofid\/ofac007"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmi.2015.01.003"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/9781555819828"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00617-21"},{"key":"e_1_3_3_22_2","unstructured":"CLSI. 2024. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically. CLSI"},{"key":"e_1_3_3_23_2","unstructured":"FDA. 2009. Guidance for industry and FDA class II special controls guidance document: antimicrobial susceptibility test (AST) systems"},{"key":"e_1_3_3_24_2","volume-title":"Evaluation of stability of in vitro medical laboratory test reagents","author":"Mark H","year":"2023","unstructured":"Mark H, Bachmann LM, Chromczak JG, Florence C, Julian D, Sheryl F, Justine F, Meng QH, Pierson-Perry JF, Michael P, Brian P, VLynn S, Tusneem NA, ARoxana V, Xiong KM. 2023. Evaluation of stability of in vitro medical laboratory test reagents. Clinical and Laboratory Standards Institute."},{"key":"e_1_3_3_25_2","volume-title":"CLSI document","author":"Wayne P","year":"2005","unstructured":"Wayne P. 2005. Interference testing in clinical chemistry; approved guideline\u2014second edition. In CLSI document, EP07-A2 ed ed. Clinical and Laboratory Standards Institute.","edition":"07"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00134-017-4683-6"},{"key":"e_1_3_3_27_2","volume-title":"Henry\u2019s clinical diagnosis and management by laboratory methods","author":"McPherson RA","year":"2016","unstructured":"McPherson RA, Ms MD, Pincus MR. 2016. Henry\u2019s clinical diagnosis and management by laboratory methods. 24th Edition. Correspondencias & An\u00e1lisis.","edition":"24"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-012-1588-8"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2017.13836"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.201609-1848OC"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.annemergmed.2021.02.006"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12879-022-07772-x"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1093\/femsre\/fux004"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.21037\/jtd.2020.01.47"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00134-013-3077-7"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1002\/phar.2201"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmi.2021.09.003"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciaa528"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jcm.01819-24","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jcm.01819-24","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T13:01:17Z","timestamp":1747227677000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/jcm.01819-24"}},"subtitle":[],"editor":[{"given":"Erin","family":"McElvania","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2025,5,14]]},"references-count":37,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2025,5,14]]}},"alternative-id":["10.1128\/jcm.01819-24"],"URL":"https:\/\/doi.org\/10.1128\/jcm.01819-24","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,5,14]]},"assertion":[{"value":"2024-11-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-02-19","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-03-31","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e01819-24"}}