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However, increasing resistance to multiple antibiotics may necessitate waiting for culture-based diagnostics to select an effective treatment. There is a need for same-day culture-free diagnostics that identify infection and detect antimicrobial resistance. We investigated if Nanopore sequencing can detect sufficient\n                    <jats:named-content content-type=\"genus-species\">Neisseria gonorrhoeae<\/jats:named-content>\n                    DNA to reconstruct whole genomes directly from urine samples.\n                  <\/jats:p>","DOI":"10.1128\/jcm.01822-19","type":"journal-article","created":{"date-parts":[[2019,12,19]],"date-time":"2019-12-19T06:35:52Z","timestamp":1576737352000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":50,"title":["Optimizing DNA Extraction Methods for Nanopore Sequencing of Neisseria gonorrhoeae Directly from Urine Samples"],"prefix":"10.1128","volume":"58","author":[{"given":"Teresa L.","family":"Street","sequence":"first","affiliation":[{"name":"Nuffield Department of Clinical Medicine, University of Oxford, John Radcliffe Hospital, Oxford, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leanne","family":"Barker","sequence":"additional","affiliation":[{"name":"Nuffield Department of Clinical Medicine, University of Oxford, John Radcliffe Hospital, Oxford, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicholas D.","family":"Sanderson","sequence":"additional","affiliation":[{"name":"Nuffield Department of Clinical Medicine, University of Oxford, John Radcliffe Hospital, Oxford, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James","family":"Kavanagh","sequence":"additional","affiliation":[{"name":"Nuffield Department of Clinical Medicine, University of Oxford, John Radcliffe Hospital, Oxford, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah","family":"Hoosdally","sequence":"additional","affiliation":[{"name":"Nuffield Department of Clinical Medicine, University of Oxford, John Radcliffe Hospital, Oxford, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin","family":"Cole","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Infection, Royal Sussex County Hospital, Brighton, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert","family":"Newnham","sequence":"additional","affiliation":[{"name":"Microbiology Laboratory, John Radcliffe Hospital, Oxford University Hospitals NHS Foundation Trust, Oxford, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mathyruban","family":"Selvaratnam","sequence":"additional","affiliation":[{"name":"Microbiology Laboratory, John Radcliffe Hospital, Oxford University Hospitals NHS Foundation Trust, Oxford, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monique","family":"Andersson","sequence":"additional","affiliation":[{"name":"Microbiology Laboratory, John Radcliffe Hospital, Oxford University Hospitals NHS Foundation Trust, Oxford, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin J.","family":"Llewelyn","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Infection, Royal Sussex County Hospital, Brighton, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Justin","family":"O\u2019Grady","sequence":"additional","affiliation":[{"name":"Quadram Institute Bioscience, Norwich, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Derrick W.","family":"Crook","sequence":"additional","affiliation":[{"name":"Nuffield Department of Clinical Medicine, University of Oxford, John Radcliffe Hospital, Oxford, United Kingdom"},{"name":"National Institute for Health Research Oxford Biomedical Research Centre, John Radcliffe Hospital, Oxford, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David W.","family":"Eyre","sequence":"additional","affiliation":[{"name":"Nuffield Department of Clinical Medicine, University of Oxford, John Radcliffe Hospital, Oxford, United Kingdom"},{"name":"National Institute for Health Research Oxford Biomedical Research Centre, John Radcliffe Hospital, Oxford, United Kingdom"},{"name":"Big Data Institute, University of Oxford, Oxford, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0143304"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12879-015-1029-2"},{"key":"e_1_3_3_4_2","volume-title":"British Association for Sexual Health and HIV national guideline for the management of infection with Neisseria gonorrhoeae","author":"Fifer H","year":"2019","unstructured":"Fifer H, Saunders J, Soni S, Sadiq T, Fitzgerald M. 2019. 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