{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T16:18:48Z","timestamp":1787242728525,"version":"build-2736575974"},"reference-count":44,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2021,1,21]],"date-time":"2021-01-21T00:00:00Z","timestamp":1611187200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2021,1,21]],"date-time":"2021-01-21T00:00:00Z","timestamp":1611187200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000057","name":"HHS | NIH | National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["GM119873"],"award-info":[{"award-number":["GM119873"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2021,1,21]]},"abstract":"<jats:p>\n                    Widely employed diagnostic antibody serology for Lyme disease, known as standard two-tier testing (STTT), exhibits insufficient sensitivity in early Lyme disease, yielding many thousands of false-negative test results each year. Given this problem, we applied serum antibody repertoire analysis (SERA), or next-generation sequencing (NGS)-based serology, to discover IgG and IgM antibody epitope motifs capable of detecting Lyme disease-specific antibodies with high sensitivity and specificity. Iterative motif discovery and bioinformatic analysis of epitope repertoires from subjects with Lyme disease (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u2009264) and controls (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u2009391) yielded a set of 28 epitope motifs representing 20 distinct IgG antibody epitopes and a set of 38 epitope motifs representing 21 distinct IgM epitopes, which performed equivalently in a large validation cohort of STTT-positive samples.\n                  <\/jats:p>","DOI":"10.1128\/jcm.01836-20","type":"journal-article","created":{"date-parts":[[2020,10,30]],"date-time":"2020-10-30T10:02:00Z","timestamp":1604052120000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":14,"title":["Serum Epitope Repertoire Analysis Enables Early Detection of Lyme Disease with Improved Sensitivity in an Expandable Multiplex Format"],"prefix":"10.1128","volume":"59","author":[{"given":"Jack","family":"Reifert","sequence":"first","affiliation":[{"name":"Serimmune Inc., Goleta, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kathy","family":"Kamath","sequence":"additional","affiliation":[{"name":"Serimmune Inc., Goleta, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joel","family":"Bozekowski","sequence":"additional","affiliation":[{"name":"Serimmune Inc., Goleta, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ewa","family":"Lis","sequence":"additional","affiliation":[{"name":"Serimmune Inc., Goleta, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth J.","family":"Horn","sequence":"additional","affiliation":[{"name":"Lyme Disease Biobank, Portland, Oregon, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dane","family":"Granger","sequence":"additional","affiliation":[{"name":"Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester Minnesota, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6886-2294","authenticated-orcid":false,"given":"Elitza S.","family":"Theel","sequence":"additional","affiliation":[{"name":"Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester Minnesota, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John","family":"Shon","sequence":"additional","affiliation":[{"name":"Serimmune Inc., Goleta, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jaymie R.","family":"Sawyer","sequence":"additional","affiliation":[{"name":"Serimmune Inc., Goleta, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4583-751X","authenticated-orcid":false,"given":"Patrick S.","family":"Daugherty","sequence":"additional","affiliation":[{"name":"Serimmune Inc., Goleta, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.15585\/mmwr.ss6622a1"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.9778\/cmajo.20120002"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/civ735"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.38756"},{"key":"e_1_3_2_6_2","unstructured":"Johnson L. 2019. 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