{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T23:48:44Z","timestamp":1791071324266,"version":"4.1.0"},"reference-count":20,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2007,4,1]],"date-time":"2007-04-01T00:00:00Z","timestamp":1175385600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2007,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:italic>Cryptococcus neoformans<\/jats:italic>\n            is a fungal pathogen that causes life-threatening infections primarily in immunocompromised hosts. Based on the genetic characteristics and serologic properties of capsular polysaccharides, three varieties and five serotypes have been defined:\n            <jats:italic>C. neoformans<\/jats:italic>\n            var.\n            <jats:italic>neoformans<\/jats:italic>\n            (serotype D),\n            <jats:italic>C. neoformans<\/jats:italic>\n            var.\n            <jats:italic>grubii<\/jats:italic>\n            (serotype A), hybrid serotype AD, and\n            <jats:italic>C. neoformans<\/jats:italic>\n            var.\n            <jats:italic>gattii<\/jats:italic>\n            (serotypes B and C). Epidemiologic features, such as geographic distribution and ecologic niche, and clinical characteristics have been shown to be associated with serotypes. At the present time, serotyping is based on agglutination tests with either commercial or \u201chomemade\u201d antisera or on immunofluorescence assays using a monoclonal antibody directed against the capsule polysaccharide. In this paper, we describe two molecular methods (PCR-restriction enzyme analysis and length polymorphism analysis) for\n            <jats:italic>C. neoformans<\/jats:italic>\n            serotype identification. Both are based on the sequence characteristics of a fragment of the\n            <jats:italic>CAP59<\/jats:italic>\n            gene required for capsule biosynthesis. Testing of 72\n            <jats:italic>C. neoformans<\/jats:italic>\n            strains including representatives of the five serotypes demonstrated the reliability of these methods.\n          <\/jats:p>","DOI":"10.1128\/jcm.01839-06","type":"journal-article","created":{"date-parts":[[2007,2,8]],"date-time":"2007-02-08T01:29:12Z","timestamp":1170898152000},"page":"1261-1265","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":39,"title":["Molecular Identification of\n            <i>Cryptococcus neoformans<\/i>\n            Serotypes"],"prefix":"10.1128","volume":"45","author":[{"given":"A.","family":"Enache-Angoulvant","sequence":"first","affiliation":[{"name":"Laboratoire de Parasitologie-Mycologie, Universite\u0301 Pierre et Marie Curie, Faculte\u0301 de Me\u0301decine Pierre et Marie Curie, site St. Antoine, F-75012 Paris, France"},{"name":"Service de Parasitologie-Mycologie, APHP, Ho\u0302pital Tenon, F-75020 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Chandenier","sequence":"additional","affiliation":[{"name":"Laboratoire de Parasitologie-Mycologie, Ho\u0302pital Bretonneau, F-37044 Tours, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.","family":"Symoens","sequence":"additional","affiliation":[{"name":"Scientific Institute of Public Health, Mycology Section, B-1050 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Lacube","sequence":"additional","affiliation":[{"name":"Laboratoire de Parasitologie-Mycologie, Universite\u0301 Pierre et Marie Curie, Faculte\u0301 de Me\u0301decine Pierre et Marie Curie, site St. Antoine, F-75012 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Bolognini","sequence":"additional","affiliation":[{"name":"Laboratoire de Parasitologie-Mycologie, Universite\u0301 Pierre et Marie Curie, Faculte\u0301 de Me\u0301decine Pierre et Marie Curie, site St. Antoine, F-75012 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Douchet","sequence":"additional","affiliation":[{"name":"Laboratoire de Parasitologie-Mycologie, Ho\u0302pital Bretonneau, F-37044 Tours, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. L.","family":"Poirot","sequence":"additional","affiliation":[{"name":"Laboratoire de Parasitologie-Mycologie, Universite\u0301 Pierre et Marie Curie, Faculte\u0301 de Me\u0301decine Pierre et Marie Curie, site St. Antoine, F-75012 Paris, France"},{"name":"Laboratoire de Parasitologie-Mycologie, APHP, Ho\u0302pital St. Antoine, F-75012 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Hennequin","sequence":"additional","affiliation":[{"name":"Laboratoire de Parasitologie-Mycologie, Universite\u0301 Pierre et Marie Curie, Faculte\u0301 de Me\u0301decine Pierre et Marie Curie, site St. Antoine, F-75012 Paris, France"},{"name":"Service de Parasitologie-Mycologie, APHP, Ho\u0302pital Tenon, F-75020 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","unstructured":"Casadevall A. and J. R. Perfect. 1998. Cryptococcus neoformans . 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