{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T14:38:59Z","timestamp":1791038339513,"version":"4.1.0"},"reference-count":26,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2009,1,1]],"date-time":"2009-01-01T00:00:00Z","timestamp":1230768000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2009,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Human infection with\n            <jats:italic>Chlamydophila<\/jats:italic>\n            (\n            <jats:italic>Chlamydia<\/jats:italic>\n            )\n            <jats:italic>psittaci<\/jats:italic>\n            can lead to psittacosis, a disease that occasionally results in severe pneumonia and other medical complications.\n            <jats:italic>C. psittaci<\/jats:italic>\n            is currently grouped into seven avian genotypes: A through F and E\/B. Serological testing, outer membrane protein A (\n            <jats:italic>ompA<\/jats:italic>\n            ) gene sequencing, and restriction fragment length polymorphism analysis are currently used for distinguishing these genotypes. Although accurate, these methods are time-consuming and require multiple confirmatory tests. By targeting the\n            <jats:italic>ompA<\/jats:italic>\n            gene, a real-time PCR assay has been developed to rapidly detect and genotype\n            <jats:italic>C. psittaci<\/jats:italic>\n            by light-upon-extension chemistry and high-resolution melt analysis. Using this assay, we screened 169 animal specimens; 98 were positive for\n            <jats:italic>C. psittaci<\/jats:italic>\n            (71.4% genotype A, 3.1% genotype B, 4.1% genotype E, and 21.4% unable to be typed). This test may provide insight into the distribution of each genotype among specific hosts and provide epidemiological and epizootiological data in human and mammalian\/avian cases. This diagnostic assay may also have veterinary applications during chlamydial outbreaks, particularly with respect to identifying the sources and tracking the movements of a particular genotype when multiple animal facilities are affected.\n          <\/jats:p>","DOI":"10.1128\/jcm.01851-08","type":"journal-article","created":{"date-parts":[[2008,11,13]],"date-time":"2008-11-13T01:58:25Z","timestamp":1226541505000},"page":"175-181","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":52,"title":["Genotyping of\n            <i>Chlamydophila psittaci<\/i>\n            by Real-Time PCR and High-Resolution Melt Analysis"],"prefix":"10.1128","volume":"47","author":[{"given":"Stephanie L.","family":"Mitchell","sequence":"first","affiliation":[{"name":"Respiratory Disease Branch, Centers for Disease Control and Prevention, Atlanta, Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernard J.","family":"Wolff","sequence":"additional","affiliation":[{"name":"Respiratory Disease Branch, Centers for Disease Control and Prevention, Atlanta, Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W. Lanier","family":"Thacker","sequence":"additional","affiliation":[{"name":"Respiratory Disease Branch, Centers for Disease Control and Prevention, Atlanta, Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paula G.","family":"Ciembor","sequence":"additional","affiliation":[{"name":"Infectious Diseases Laboratory, College of Veterinary Medicine, University of Georgia, Athens, Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher R.","family":"Gregory","sequence":"additional","affiliation":[{"name":"Infectious Diseases Laboratory, College of Veterinary Medicine, University of Georgia, Athens, Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karin D. E.","family":"Everett","sequence":"additional","affiliation":[{"name":"Ameritek USA, Everett, Washington"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Branson W.","family":"Ritchie","sequence":"additional","affiliation":[{"name":"Infectious Diseases Laboratory, College of Veterinary Medicine, University of Georgia, Athens, Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonas M.","family":"Winchell","sequence":"additional","affiliation":[{"name":"Respiratory Disease Branch, Centers for Disease Control and Prevention, Atlanta, Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-007-0431-0"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-51-1-203"},{"key":"e_1_3_2_4_2","first-page":"3","volume":"49","year":"2000","unstructured":"Centers for Disease Control and Prevention. 2000. 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Compendium of measures to control Chlamydophila psittaci infection among humans (psittacosis) and pet birds (avian chlamydiosis) 2008. http:\/\/www.nasphv.org\/Documents\/Psittacosis.pdf\n."},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tvjl.2008.02.025"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-8-63"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.6.3011-3013.2005"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/0923-2508(96)80893-X"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.2460\/javma.2005.226.532"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid1103.040601"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.2.575-583.2002"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.02329-0"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0923-2508(97)81588-4"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.66.11.5494-5500.1998"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01153-07"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00582-08"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.01851-08","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.01851-08","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T06:40:08Z","timestamp":1646462408000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.01851-08"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,1]]},"references-count":26,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2009,1]]}},"alternative-id":["10.1128\/JCM.01851-08"],"URL":"https:\/\/doi.org\/10.1128\/jcm.01851-08","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,1]]},"assertion":[{"value":"2008-09-24","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2008-10-31","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2009-01-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}