{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T03:55:11Z","timestamp":1792641311067,"version":"4.5.0"},"reference-count":19,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2013,11,1]],"date-time":"2013-11-01T00:00:00Z","timestamp":1383264000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2013,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Detection of\n            <jats:named-content content-type=\"genus-species\">Histoplasma capsulatum<\/jats:named-content>\n            urinary antigen (UAg) is important for the initial diagnosis of infection and for monitoring of patient responses to antifungal therapy. This study evaluated an analyte-specific reagent (ASR) enzyme immunoassay (EIA) for the detection of\n            <jats:named-content content-type=\"genus-species\">H. capsulatum<\/jats:named-content>\n            UAg from Immuno Mycologics, Inc. (IMMY) (Norman, OK) in comparison with routine testing with the MiraVista (MVista)\n            <jats:named-content content-type=\"genus-species\">H. capsulatum<\/jats:named-content>\n            quantitative EIA (MiraVista Diagnostics, Indianapolis, IN). Using prospectively collected urine specimens (\n            <jats:italic>n<\/jats:italic>\n            = 1,003), we observed an overall percent agreement between the two assays of 97.6% (979\/1,003 samples). Compared with the MVista EIA, the sensitivity and specificity of the IMMY ASR EIA were 64.5% (40\/62 samples) and 99.8% (939\/941 samples), respectively, using a cutoff value of 0.5 ng\/ml. Based on available clinical histories for 23\/24 discordant samples, 5 IMMY assay-negative\/MVista assay-positive samples were considered falsely positive. Furthermore, 10\/23 discordant samples were positive by the MVista EIA but were below the limit of quantitation (&lt;0.4 ng\/ml). The clinical significance of these low positive results in the MVista EIA is unclear. In addition to the prospective study, we tested 11 urine specimens collected from patients with culture-confirmed\n            <jats:named-content content-type=\"genus-species\">Histoplasma<\/jats:named-content>\n            infections, and 100% (11\/11 samples) were positive by the IMMY ASR EIA. In conclusion, the IMMY ASR EIA may offer an alternative approach for the detection of\n            <jats:named-content content-type=\"genus-species\">Histoplasma<\/jats:named-content>\n            UAg. Additional prospective studies are needed to better characterize the performance of the IMMY ASR EIA in conjunction with clinical and laboratory findings.\n          <\/jats:p>","DOI":"10.1128\/jcm.01868-13","type":"journal-article","created":{"date-parts":[[2013,8,22]],"date-time":"2013-08-22T04:05:55Z","timestamp":1377144355000},"page":"3555-3559","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":50,"title":["Evaluation of an Enzyme Immunoassay for Detection of Histoplasma capsulatum Antigen from Urine Specimens"],"prefix":"10.1128","volume":"51","author":[{"given":"Elitza S.","family":"Theel","sequence":"first","affiliation":[{"name":"Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deborah J.","family":"Jespersen","sequence":"additional","affiliation":[{"name":"Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julie","family":"Harring","sequence":"additional","affiliation":[{"name":"Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jay","family":"Mandrekar","sequence":"additional","affiliation":[{"name":"Division of Biomedical Statistics and Informatics, Department of Health Sciences and Research, Mayo Clinic, Rochester, Minnesota, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew J.","family":"Binnicker","sequence":"additional","affiliation":[{"name":"Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid1709.101987"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1086\/521259"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1517\/14712598.6.11.1207"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccm.2009.02.002"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00027-06"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2003.08.007"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-97-5-680"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cir435"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00673-11"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccm.2009.02.011"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00389-10"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9343(92)90106-L"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1086\/513647"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00071-07"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00035-07"},{"key":"e_1_3_3_17_2","volume-title":"Abstr. 113th Gen. Meet. Am. Soc. Microbiol.","author":"Jespersen DJ","year":"2013","unstructured":"JespersenDJHarringJMandrekarMBinnickerMJTheelES. 2013. Evaluation of a Histoplasma capsulatum galactomannan antigen enzyme immunoassay, abstr C-90\/929. Abstr. 113th Gen. Meet. Am. Soc. Microbiol. American Society for Microbiology, Washington, DC."},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1148\/radiology.148.3.6878708"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1002\/0471722146"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-3062.2006.00165.x"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.01868-13","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.01868-13","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T11:32:38Z","timestamp":1646479958000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.01868-13"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,11]]},"references-count":19,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2013,11]]}},"alternative-id":["10.1128\/JCM.01868-13"],"URL":"https:\/\/doi.org\/10.1128\/jcm.01868-13","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,11]]},"assertion":[{"value":"2013-07-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-08-09","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-10-16","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}