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We describe four multiplex PCRs (mPCRs), designated VDL05, VDL06, VDL07, and VDL09, which facilitate the detection of a wide range of agents either known to be or putatively associated with cervicitis, including cytomegalovirus (CMV), enterovirus (EV), Epstein-Barr virus (EBV), varicella-zoster virus (VZV), herpes simplex virus type 1 (HSV-1), and herpes simplex virus type 2 (HSV-2) (VDL05);<jats:italic>Ureaplasma parvum<\/jats:italic>,<jats:italic>Ureaplasma urealyticum<\/jats:italic>,<jats:italic>Mycoplasma genitalium<\/jats:italic>, and<jats:italic>Mycoplasma hominis<\/jats:italic>(VDL06);<jats:italic>Chlamydia trachomatis<\/jats:italic>,<jats:italic>Trichomonas vaginalis<\/jats:italic>,<jats:italic>Treponema pallidum<\/jats:italic>, and group B streptococci (VDL07); and adenovirus species A to E (VDL09). The mPCRs were used to test 233 cervical swabs from 175 women attending a sexual-health clinic in Sydney, Australia, during 2006 and 2007. The agents detected alone or in combination in all cervical swabs (percentage of total swabs) included CMV (6.0), EV (2.1), EBV (2.6), VZV (4.7), HSV-1 (2.6), HSV-2 (0.8), HSV-2 and VZV (0.4),<jats:italic>U. parvum<\/jats:italic>(57.0),<jats:italic>U. urealyticum<\/jats:italic>(6.1),<jats:italic>M. genitalium<\/jats:italic>(1.3),<jats:italic>M. hominis<\/jats:italic>(13.7),<jats:italic>C. trachomatis<\/jats:italic>(0.4),<jats:italic>T. vaginalis<\/jats:italic>(3.4), and group B streptococci (0.4). Adenovirus species A to E and<jats:italic>T. pallidum<\/jats:italic>were not detected. These assays are adaptable for routine diagnostic laboratories and provide an opportunity to measure the true prevalence of microorganisms potentially associated with cervicitis and other genital infections.<\/jats:p>","DOI":"10.1128\/jcm.01873-08","type":"journal-article","created":{"date-parts":[[2009,3,5]],"date-time":"2009-03-05T02:37:02Z","timestamp":1236220622000},"page":"1358-1363","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":56,"title":["Multiplex PCR Testing Detection of Higher-than-Expected Rates of Cervical<i>Mycoplasma<\/i>,<i>Ureaplasma<\/i>, and<i>Trichomonas<\/i>and Viral Agent Infections in Sexually Active Australian Women"],"prefix":"10.1128","volume":"47","author":[{"given":"Christopher J.","family":"McIver","sequence":"first","affiliation":[{"name":"Virology Division, Microbiology Department (SEALS), Prince of Wales Hospital"},{"name":"School of Medical Sciences"},{"name":"School of Biotechnology and Biomolecular Sciences, University of New South Wales"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nikolas","family":"Rismanto","sequence":"additional","affiliation":[{"name":"Virology Division, Microbiology Department (SEALS), Prince of Wales Hospital"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine","family":"Smith","sequence":"additional","affiliation":[{"name":"Virology Division, Microbiology Department (SEALS), Prince of Wales Hospital"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zin Wai","family":"Naing","sequence":"additional","affiliation":[{"name":"Virology Division, Microbiology Department (SEALS), Prince of Wales Hospital"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ben","family":"Rayner","sequence":"additional","affiliation":[{"name":"Virology Division, Microbiology Department (SEALS), Prince of Wales Hospital"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. 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