{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,7]],"date-time":"2026-03-07T21:36:41Z","timestamp":1772919401550,"version":"3.50.1"},"reference-count":36,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T00:00:00Z","timestamp":1747180800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T00:00:00Z","timestamp":1747180800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["1R01AI143653-01A1)"],"award-info":[{"award-number":["1R01AI143653-01A1)"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R01 AI61019"],"award-info":[{"award-number":["R01 AI61019"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["U19 AI061972"],"award-info":[{"award-number":["U19 AI061972"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2025,5,14]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:sec>\n            <jats:title\/>\n            <jats:p>\n              <jats:italic toggle=\"yes\">Mycoplasma genitalium<\/jats:italic>\n              is a sexually transmitted pathogen that can cause a range of reproductive tract diseases in both men and women. To disentangle the relationship between\n              <jats:italic toggle=\"yes\">M. genitalium<\/jats:italic>\n              infection(s) and subsequent reproductive health complications at the population level, accurate serological tools are needed. The major challenge in developing specific\n              <jats:italic toggle=\"yes\">M. genitalium<\/jats:italic>\n              serological tests is the extensive cross-reactivity with the closely related ubiquitous respiratory tract pathogen,\n              <jats:italic toggle=\"yes\">Mycoplasma pneumoniae<\/jats:italic>\n              . In this report, we describe the development of an immunoblot assay based on a recombinant fragment of the\n              <jats:italic toggle=\"yes\">M. genitalium<\/jats:italic>\n              MG075 protein present in lipid-associated membrane extracts. A sensitivity of 87.1% was achieved based on the testing antibody responses in sera from 101 adults with PCR-confirmed\n              <jats:italic toggle=\"yes\">M. genitalium<\/jats:italic>\n              infection. A specificity of 95.2% was obtained through the evaluation of sera from 166 children under 15 years of age with and without\n              <jats:italic toggle=\"yes\">M. pneumoniae<\/jats:italic>\n              infection, who were unlikely to have been exposed to sexually transmitted\n              <jats:italic toggle=\"yes\">M. genitalium<\/jats:italic>\n              . The development of a serological assay capable of accurately distinguishing\n              <jats:italic toggle=\"yes\">M. genitalium<\/jats:italic>\n              and\n              <jats:italic toggle=\"yes\">M. pneumoniae<\/jats:italic>\n              will enable a better understanding of associations between\n              <jats:italic toggle=\"yes\">M. genitalium<\/jats:italic>\n              and adverse reproductive sequelae.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>IMPORTANCE<\/jats:title>\n            <jats:p>\n              <jats:italic toggle=\"yes\">Mycoplasma genitalium<\/jats:italic>\n              is the second most common sexually transmitted bacterial infection after chlamydia. The long-term consequences of the infection are still under investigation, but reliable tools for monitoring exposure by detection of antibodies have been lacking specificity due to the presence of cross-reacting antibodies to the closely related\n              <jats:italic toggle=\"yes\">Mycoplasma pneumoniae<\/jats:italic>\n              . Here, we describe a novel diagnostic antigen with promising sensitivity (87%) and specificity (95%) based on testing of sera from patients with PCR-confirmed\n              <jats:italic toggle=\"yes\">M. genitalium<\/jats:italic>\n              infection and children with and without\n              <jats:italic toggle=\"yes\">M. pneumoniae<\/jats:italic>\n              infection, respectively. The MG075F1 antigen was expressed in\n              <jats:italic toggle=\"yes\">Escherichia coli<\/jats:italic>\n              , and the recombinant antigen was used in a western line-blot. Due to the insolubility of the antigen, harsh denaturing conditions were needed, making an enzyme-linked immunosorbent assay (ELISA) format impossible. Future work should explore shorter fragments or protein engineering to allow for assay designs better suited for high-throughput screening.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/jcm.01876-24","type":"journal-article","created":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T13:04:24Z","timestamp":1745413464000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":1,"title":["Detection of human IgG antibodies against\n            <i>Mycoplasma genitalium<\/i>\n            using a recombinant MG075 antigen"],"prefix":"10.1128","volume":"63","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5150-1153","authenticated-orcid":true,"given":"Anna Marie Overgaard","family":"Kildemoes","sequence":"first","affiliation":[{"name":"Reproductive Microbiology, Department of Bacteria, Parasites & Fungi, Statens Serum Institut","place":["Copenhagen, Denmark"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olivia Sureya Seier\u00f8","family":"Rai","sequence":"additional","affiliation":[{"name":"Reproductive Microbiology, Department of Bacteria, Parasites & Fungi, Statens Serum Institut","place":["Copenhagen, Denmark"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elisabeth Probst Lyng","family":"Westermann","sequence":"additional","affiliation":[{"name":"Reproductive Microbiology, Department of Bacteria, Parasites & Fungi, Statens Serum Institut","place":["Copenhagen, Denmark"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Henrik Frederik Bekkevold","family":"Johansen","sequence":"additional","affiliation":[{"name":"Reproductive Microbiology, Department of Bacteria, Parasites & Fungi, Statens Serum Institut","place":["Copenhagen, Denmark"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eva","family":"Bj\u00f8rnelius","sequence":"additional","affiliation":[{"name":"Department of Dermatovenereology, Karolinska University Hospital Huddinge","place":["Stockholm, Sweden"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carin","family":"Anagrius","sequence":"additional","affiliation":[{"name":"Department of Venereology","place":["Falu lasarett, Sweden"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liang","family":"Ma","sequence":"additional","affiliation":[{"name":"Critical Care Medicine Department, NIH Clinical Center, National Institutes of Health","place":["Bethesda, USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ida","family":"Rosenkrands","sequence":"additional","affiliation":[{"name":"Infectious Disease Immunology, Center for vaccine research, Statens Serum Institut","place":["Copenhagen, 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