{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T02:21:57Z","timestamp":1790821317711,"version":"4.1.0"},"reference-count":19,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2017,3,1]],"date-time":"2017-03-01T00:00:00Z","timestamp":1488326400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2017,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>During measles outbreaks, it is important to be able to rapidly distinguish between measles cases and vaccine reactions to avoid unnecessary outbreak response measures such as case isolation and contact investigations. We have developed a real-time reverse transcription-PCR (RT-PCR) method specific for genotype A measles virus (MeV) (MeVA RT-quantitative PCR [RT-qPCR]) that can identify measles vaccine strains rapidly, with high throughput, and without the need for sequencing to determine the genotype. We have evaluated the method independently in three measles reference laboratories using two platforms, the Roche LightCycler 480 system and the Applied Biosystems (ABI) 7500 real-time PCR system. In comparison to the standard real-time RT-PCR method, the MeVA RT-qPCR showed 99.5% specificity for genotype A and 94% sensitivity for both platforms. The new assay was able to detect RNA from five currently used vaccine strains, AIK-C, CAM-70, Edmonston-Zagreb, Moraten, and Shanghai-191. The MeVA RT-qPCR assay has been used successfully for measles surveillance in reference laboratories, and it could be readily deployed to national and subnational laboratories on a wide scale.<\/jats:p>","DOI":"10.1128\/jcm.01879-16","type":"journal-article","created":{"date-parts":[[2016,11,17]],"date-time":"2016-11-17T04:10:30Z","timestamp":1479355830000},"page":"735-743","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":57,"title":["Rapid Identification of Measles Virus Vaccine Genotype by Real-Time PCR"],"prefix":"10.1128","volume":"55","author":[{"given":"Felicia","family":"Roy","sequence":"first","affiliation":[{"name":"Viral Exanthemata and STD Section, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lillian","family":"Mendoza","sequence":"additional","affiliation":[{"name":"Viral Exanthemata and STD Section, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joanne","family":"Hiebert","sequence":"additional","affiliation":[{"name":"Viral Exanthemata and STD Section, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rebecca J.","family":"McNall","sequence":"additional","affiliation":[{"name":"Division of Viral Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bettina","family":"Bankamp","sequence":"additional","affiliation":[{"name":"Division of Viral Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah","family":"Connolly","sequence":"additional","affiliation":[{"name":"Division of Viral Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA"},{"name":"Emory University, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amy","family":"L\u00fcdde","sequence":"additional","affiliation":[{"name":"Unit Measles, Mumps, Rubella, and Viruses Affecting Immunocompromised Patients, Robert Koch Institute, Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicole","family":"Friedrich","sequence":"additional","affiliation":[{"name":"Unit Measles, Mumps, Rubella, and Viruses Affecting Immunocompromised Patients, Robert Koch Institute, Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annette","family":"Mankertz","sequence":"additional","affiliation":[{"name":"Unit Measles, Mumps, Rubella, and Viruses Affecting Immunocompromised Patients, Robert Koch Institute, Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul A.","family":"Rota","sequence":"additional","affiliation":[{"name":"Division of Viral Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alberto","family":"Severini","sequence":"additional","affiliation":[{"name":"Viral Exanthemata and STD Section, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada"},{"name":"Dept. of Medical Microbiology, Faculty of Health Sciences, University of Manitoba, Winnipeg, Manitoba, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jir111"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1093\/ofid\/ofv048"},{"key":"e_1_3_3_4_2","first-page":"153","article-title":"Measles outbreak\u2013California, December 2014-February 2015","volume":"64","author":"Zipprich J","year":"2015","unstructured":"Zipprich J Winter K Hacker J Xia D Watt J Harriman K , Centers for Disease Control and Prevention (CDC). 2015. Measles outbreak\u2013California, December 2014-February 2015. MMWR Morb Mortal Wkly Rep64:153\u2013154.","journal-title":"MMWR Morb Mortal Wkly Rep"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tmaid.2014.10.022"},{"key":"e_1_3_3_6_2","first-page":"373","article-title":"Measles - United States, January 4\u2013April 2, 2015","volume":"64","author":"Clemmons NS","year":"2015","unstructured":"Clemmons NS Gastanaduy PA Fiebelkorn AP Redd SB Wallace GS , Centers for Disease Control and Prevention (CDC). 2015. Measles - United States, January 4\u2013April 2, 2015. MMWR Morb Mortal Wkly Rep64:373\u2013376.","journal-title":"MMWR Morb Mortal Wkly Rep"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2015.07.027"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid2109.150391"},{"key":"e_1_3_3_9_2","first-page":"ACS-3","article-title":"Guidelines for the prevention and control of measles outbreaks in Canada","volume":"39","author":"Measles and Rubella Elimination Working Group","year":"2013","unstructured":"Measles and Rubella Elimination Working Group. 2013. Guidelines for the prevention and control of measles outbreaks in Canada. Can Commun Dis Rep39:ACS-3.","journal-title":"Can Commun Dis Rep"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1525-1470.2005.22208.x"},{"key":"e_1_3_3_11_2","first-page":"265","article-title":"Expanded Programme on Immunization (EPI). Standardization of the nomenclature for describing the genetic characteristics of wild-type measles viruses","volume":"73","year":"1998","unstructured":". 1998. Expanded Programme on Immunization (EPI). Standardization of the nomenclature for describing the genetic characteristics of wild-type measles viruses. Wkly Epidemiol Rec73:265\u2013269.","journal-title":"Wkly Epidemiol Rec"},{"key":"e_1_3_3_12_2","first-page":"373","article-title":"Genetic diversity of wild-type measles viruses and the global measles nucleotide surveillance database (MeaNS)","volume":"90","year":"2015","unstructured":". 2015. Genetic diversity of wild-type measles viruses and the global measles nucleotide surveillance database (MeaNS). Wkly Epidemiol Rec90:373\u2013380.","journal-title":"Wkly Epidemiol Rec"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmii.2015.10.004"},{"key":"e_1_3_3_14_2","unstructured":"Lowry\nR\n. VassarStats: website for statistical computation. http:\/\/vassarstats.net."},{"key":"e_1_3_3_15_2","first-page":"474","article-title":"Global distribution of measles and rubella genotypes\u2013update","volume":"81","year":"2006","unstructured":". 2006. Global distribution of measles and rubella genotypes\u2013update. Wkly Epidemiol Rec81:474\u2013479.","journal-title":"Wkly Epidemiol Rec"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/0168-1702(94)90025-6"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2005.10.006"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-7799(02)00038-0"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.2807\/1560-7917.ES2013.18.49.20649"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1093\/pch\/17.4.e32"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.01879-16","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.01879-16","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T10:07:26Z","timestamp":1646474846000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.01879-16"}},"subtitle":[],"editor":[{"given":"Yi-Wei","family":"Tang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2017,3]]},"references-count":19,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2017,3]]}},"alternative-id":["10.1128\/JCM.01879-16"],"URL":"https:\/\/doi.org\/10.1128\/jcm.01879-16","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,3]]},"assertion":[{"value":"2016-09-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-11-07","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-02-22","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}