{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,22]],"date-time":"2026-03-22T18:04:21Z","timestamp":1774202661144,"version":"3.50.1"},"reference-count":30,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2020,4,23]],"date-time":"2020-04-23T00:00:00Z","timestamp":1587600000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["5R44AI055229"],"award-info":[{"award-number":["5R44AI055229"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["5R44AI058375"],"award-info":[{"award-number":["5R44AI058375"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009139","name":"Deutsches Zentrum f\u00fcr Infektionsforschung","doi-asserted-by":"publisher","award":["TTU03.702"],"award-info":[{"award-number":["TTU03.702"]}],"id":[{"id":"10.13039\/100009139","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2020,4,23]]},"abstract":"<jats:p>\n            Microscopy and rapid diagnostic tests (RDTs) are the main diagnostic tools for malaria but fail to detect low-density parasitemias that are important for maintaining malaria transmission. To complement existing diagnostic methods, an isothermal reverse transcription-recombinase polymerase amplification and lateral flow assay (RT-RPA) was developed. We compared the performance with that of ultrasensitive reverse transcription-quantitative PCR (uRT-qPCR) using nucleic acid extracts from blood samples (\n            <jats:italic>n<\/jats:italic>\n            \u2009=\u2009114) obtained after standardized controlled human malaria infection (CHMI) with\n            <jats:named-content content-type=\"genus-species\">Plasmodium falciparum<\/jats:named-content>\n            sporozoites.\n          <\/jats:p>","DOI":"10.1128\/jcm.01879-19","type":"journal-article","created":{"date-parts":[[2020,2,24]],"date-time":"2020-02-24T12:05:51Z","timestamp":1582545951000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":36,"title":["Recombinase Polymerase Amplification and Lateral Flow Assay for Ultrasensitive Detection of Low-Density Plasmodium falciparum Infection from Controlled Human Malaria Infection Studies and Naturally Acquired Infections"],"prefix":"10.1128","volume":"58","author":[{"given":"Albert","family":"Lalremruata","sequence":"first","affiliation":[{"name":"Institut f\u00fcr Tropenmedizin, Universit\u00e4t T\u00fcbingen and German Center for Infection Research, T\u00fcbingen, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"The Trong","family":"Nguyen","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Tropenmedizin, Universit\u00e4t T\u00fcbingen and German Center for Infection Research, T\u00fcbingen, Germany"},{"name":"Vietnamese\u2014German Center of Excellence in Medical Research, Hanoi, Vietnam"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matthew B. B.","family":"McCall","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Tropenmedizin, Universit\u00e4t T\u00fcbingen and German Center for Infection Research, T\u00fcbingen, Germany"},{"name":"Centre de Recherches M\u00e9dicales de Lambar\u00e9n\u00e9 and African Partner Institution, German Center for Infection Research, Lambar\u00e9n\u00e9, Gabon"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ghyslain","family":"Mombo-Ngoma","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Tropenmedizin, Universit\u00e4t T\u00fcbingen and German Center for Infection Research, T\u00fcbingen, Germany"},{"name":"Centre de Recherches M\u00e9dicales de Lambar\u00e9n\u00e9 and African Partner Institution, German Center for Infection Research, Lambar\u00e9n\u00e9, Gabon"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Selidji T.","family":"Agnandji","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Tropenmedizin, Universit\u00e4t T\u00fcbingen and German Center for Infection Research, T\u00fcbingen, Germany"},{"name":"Centre de Recherches M\u00e9dicales de Lambar\u00e9n\u00e9 and African Partner Institution, German Center for Infection Research, Lambar\u00e9n\u00e9, Gabon"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ay\u00f4la A.","family":"Adegnika","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Tropenmedizin, Universit\u00e4t T\u00fcbingen and German Center for Infection Research, T\u00fcbingen, Germany"},{"name":"Centre de Recherches M\u00e9dicales de Lambar\u00e9n\u00e9 and African Partner Institution, German Center for Infection Research, Lambar\u00e9n\u00e9, Gabon"},{"name":"Department of Parasitology, Leiden University Medical Center, Leiden, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bertrand","family":"Lell","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Tropenmedizin, Universit\u00e4t T\u00fcbingen and German Center for Infection Research, T\u00fcbingen, Germany"},{"name":"Centre de Recherches M\u00e9dicales de Lambar\u00e9n\u00e9 and African Partner Institution, German Center for Infection Research, Lambar\u00e9n\u00e9, Gabon"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Ramharter","sequence":"additional","affiliation":[{"name":"Centre de Recherches M\u00e9dicales de Lambar\u00e9n\u00e9 and African Partner Institution, German Center for Infection Research, Lambar\u00e9n\u00e9, Gabon"},{"name":"Department of Tropical Medicine, Bernhard Nocht Institute for Tropical Medicine, University Medical Center Hamburg-Eppendorf, Hamburg, Germany"},{"name":"Department of Medicine, University Medical Center Hamburg-Eppendorf, Hamburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephen L.","family":"Hoffman","sequence":"additional","affiliation":[{"name":"Sanaria Inc., Rockville, Maryland, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter G.","family":"Kremsner","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Tropenmedizin, Universit\u00e4t T\u00fcbingen and German Center for Infection Research, T\u00fcbingen, Germany"},{"name":"Centre de Recherches M\u00e9dicales de Lambar\u00e9n\u00e9 and African Partner Institution, German Center for Infection Research, Lambar\u00e9n\u00e9, Gabon"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Benjamin","family":"Mordm\u00fcller","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Tropenmedizin, Universit\u00e4t T\u00fcbingen and German Center for Infection Research, T\u00fcbingen, Germany"},{"name":"Centre de Recherches M\u00e9dicales de Lambar\u00e9n\u00e9 and African Partner Institution, German Center for Infection Research, Lambar\u00e9n\u00e9, Gabon"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","volume-title":"Policy recommendation on malaria diagnostics in low transmission settings","author":"World Health Organization","year":"2014","unstructured":"World Health Organization. 2014. Policy recommendation on malaria diagnostics in low transmission settings. World Health Organization, Geneva, Switzerland."},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12936-016-1434-z"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(18)30177-4"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12936-015-0541-6"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature21060"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(17)30139-1"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.1002455"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.1002452"},{"key":"e_1_3_2_10_2","volume-title":"Last Modif 1","author":"World Health Organization","year":"2018","unstructured":"World Health Organization. 2018. Intermittent preventive treatment in pregnancy (IPTp). Last Modif 1. World Health Organization, Geneva, Switzerland."},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(18)30064-1"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.0040204"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciy1087"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1111\/tmi.13357"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12936-015-0628-0"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1586\/14737159.2014.915748"},{"key":"e_1_3_2_17_2","volume-title":"Bridging the gaps in malaria R&D: an analysis of funding\u2014from basic research and product development to research for implementation","author":"PATH","year":"2018","unstructured":"PATH. 2018. Bridging the gaps in malaria R&D: an analysis of funding\u2014from basic research and product development to research for implementation. PATH, Seattle, WA."},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12936-016-1454-8"},{"key":"e_1_3_2_19_2","volume-title":"Mass drug administration, mass screening and treatment and focal screening and treatment for malaria","author":"World Health Organization","year":"2015","unstructured":"World Health Organization. 2015. Mass drug administration, mass screening and treatment and focal screening and treatment for malaria. World Health Organization, Geneva, Switzerland."},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12879-015-0887-y"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/s1471-4922(03)00091-6"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1186\/1475-2875-11-29"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.1001942"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2007.07.057"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.17-0245"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(18)30411-0"},{"key":"e_1_3_2_27_2","unstructured":"Mpina MG Raso J Deal A Pupu LA Nyakarungu EL Davis M. d C Schindler T Urbano V Mtoro A Hamad A Lopez MSA Pasialo BN. 2019 Sensitivity and specificity of a novel highly sensitive rapid diagnostic test for detecting low density Plasmodium falciparum infection during a controlled human malaria infection study in Equatorial Guinea abstr P-292 p 89 Abstr ASTMH 68th Annual Meeting. National Harbor MD."},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1080\/14737159.2018.1431529"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.17-0225"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0169037"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12936-019-2979-4"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.01879-19","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.01879-19","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T10:46:49Z","timestamp":1646477209000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.01879-19"}},"subtitle":[],"editor":[{"given":"Bobbi S.","family":"Pritt","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2020,4,23]]},"references-count":30,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2020,4,23]]}},"alternative-id":["10.1128\/JCM.01879-19"],"URL":"https:\/\/doi.org\/10.1128\/jcm.01879-19","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,4,23]]},"assertion":[{"value":"2019-11-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-02-06","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-04-23","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e01879-19"}}