{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T01:19:24Z","timestamp":1792113564196,"version":"4.4.0"},"reference-count":32,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2018,4,1]],"date-time":"2018-04-01T00:00:00Z","timestamp":1522540800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31570015"],"award-info":[{"award-number":["31570015"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2018,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            This study aimed to validate the effectiveness of matrix-assisted laser desorption ionization\u2013time of flight mass spectrometry (MALDI-TOF MS)-based identification of filamentous fungi of the order Mucorales. A total of 111 isolates covering six genera preserved at the Research Center for Medical Mycology of Peking University were selected for MALDI-TOF MS analysis. We emphasized the study of 23 strains of\n            <jats:named-content content-type=\"genus-species\">Mucor irregularis<\/jats:named-content>\n            predominantly isolated from patients in China. We first used the Bruker Filamentous Fungi library (v1.0) to identify all 111 isolates. To increase the identification rate, we created a compensatory in-house database, the Beijing Medical University (BMU) database, using 13 reference strains covering 6 species, including\n            <jats:named-content content-type=\"genus-species\">M. irregularis<\/jats:named-content>\n            ,\n            <jats:named-content content-type=\"genus-species\">Mucor hiemalis<\/jats:named-content>\n            ,\n            <jats:named-content content-type=\"genus-species\">Mucor racemosus<\/jats:named-content>\n            ,\n            <jats:named-content content-type=\"genus-species\">Cunninghamella bertholletiae<\/jats:named-content>\n            ,\n            <jats:named-content content-type=\"genus-species\">Cunninghamella phaeospora<\/jats:named-content>\n            , and\n            <jats:named-content content-type=\"genus-species\">Cunninghamella echinulata<\/jats:named-content>\n            . All 111 isolates were then identified by MALDI-TOF MS using a combination of the Bruker library and BMU database. MALDI-TOF MS identified 55 (49.5%) and 74 (66.7%) isolates at the species and genus levels, respectively, using the Bruker Filamentous Fungi library v1.0 alone. A combination of the Bruker library and BMU database allowed MALDI-TOF MS to identify 90 (81.1%) and 111 (100%) isolates at the species and genus levels, respectively, with a significantly increased accuracy rate. MALDI-TOF MS poorly identified Mucorales when the Bruker library was used alone due to its lack of some fungal species. In contrast, this technique perfectly identified\n            <jats:named-content content-type=\"genus-species\">M. irregularis<\/jats:named-content>\n            after main spectrum profiles (MSPs) of relevant reference strains were added to the Bruker library. With an expanded Bruker library, MALDI-TOF MS is an effective tool for the identification of pathogenic Mucorales.\n          <\/jats:p>","DOI":"10.1128\/jcm.01886-17","type":"journal-article","created":{"date-parts":[[2018,2,2]],"date-time":"2018-02-02T15:26:01Z","timestamp":1517585161000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":40,"title":["Species Identification and Delineation of Pathogenic Mucorales by Matrix-Assisted Laser Desorption Ionization\u2013Time of Flight Mass Spectrometry"],"prefix":"10.1128","volume":"56","author":[{"given":"Jin","family":"Shao","sequence":"first","affiliation":[{"name":"Department of Dermatology, Peking University First Hospital, Beijing, China"},{"name":"Beijing Key Laboratory of Molecular Diagnosis on Dermatoses, Beijing, China"},{"name":"Research Center for Medical Mycology, Peking University, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhe","family":"Wan","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Peking University First Hospital, Beijing, China"},{"name":"Beijing Key Laboratory of Molecular Diagnosis on Dermatoses, Beijing, China"},{"name":"Research Center for Medical Mycology, Peking University, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruoyu","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Peking University First Hospital, Beijing, China"},{"name":"Beijing Key Laboratory of Molecular Diagnosis on Dermatoses, Beijing, China"},{"name":"Research Center for Medical Mycology, Peking University, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Peking University First Hospital, Beijing, China"},{"name":"Beijing Key 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