{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T13:19:56Z","timestamp":1789910396658,"version":"4.0.1"},"reference-count":16,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2013,12,1]],"date-time":"2013-12-01T00:00:00Z","timestamp":1385856000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2013,12]]},"abstract":"ABSTRACT<\/jats:title>\n \n The Verigene Gram-positive blood culture (BC-GP) assay (Nanosphere, Northbrook, IL) is a molecular method for the rapid identification of Gram-positive organisms and resistance markers directly from blood culture bottles. A total of 148 VersaTREK REDOX 1 40-ml aerobic bottles demonstrating Gram-positive bacteria were tested. Results were compared with those from conventional biochemical and matrix-assisted laser desorption ionization\u2013time of flight (MALDI-TOF) identifications. We obtained isolates of methicillin-resistant\n Staphylococcus aureus<\/jats:named-content>\n (MRSA) (24), methicillin-susceptible\n Staphylococcus aureus<\/jats:named-content>\n (MSSA) (14), methicillin-resistant\n Staphylococcus epidermidis<\/jats:named-content>\n (MRSE) (17), methicillin-susceptible\n Staphylococcus epidermidis<\/jats:named-content>\n (MSSE) (9), other coagulase-negative staphylococci (19),\n Streptococcus salivarius<\/jats:named-content>\n (5),\n Streptococcus parasanguinis<\/jats:named-content>\n (2),\n Streptococcus sanguinis<\/jats:named-content>\n (1),\n Streptococcus cristatus<\/jats:named-content>\n (1), the\n Streptococcus bovis<\/jats:named-content>\n group (5),\n Streptococcus agalactiae<\/jats:named-content>\n (9), the\n Streptococcus anginosus<\/jats:named-content>\n group (1),\n Streptococcus pneumoniae<\/jats:named-content>\n (6), vancomycin-resistant\n Enterococcus faecium<\/jats:named-content>\n (VRE FCM) (16), vancomycin-susceptible\n Enterococcus faecalis<\/jats:named-content>\n (3),\n Aerococcus viridans<\/jats:named-content>\n (2),\n Bacillus<\/jats:named-content>\n (6),\n Corynebacterium<\/jats:named-content>\n (8),\n Lactobacillus<\/jats:named-content>\n (2),\n Micrococcus<\/jats:named-content>\n (2),\n Neisseria mucosa<\/jats:named-content>\n (1),\n Escherichia coli<\/jats:named-content>\n (3),\n Candida tropicalis<\/jats:named-content>\n (1),\n Propionibacterium<\/jats:named-content>\n (1), and\n Rothia<\/jats:named-content>\n (1). Overall agreement with the culture results was 95%. A total of 137 of 138 (99%) monomicrobial cultures were concordant. We tested 9 polymicrobial samples and found 33% agreement. A chart review of 31 patients with MRSA, MSSA, or VRE demonstrated that the Nanosphere BC-GP assay might have led to more appropriate antibiotic selection for these patients an average of 42 h earlier. Additionally, contact isolation could have been initiated an average of 37 h earlier for patients with MRSA or VRE. The BC-GP assay may have a positive impact on patient care, health care costs, and antibiotic stewardship.\n <\/jats:p>","DOI":"10.1128\/jcm.01889-13","type":"journal-article","created":{"date-parts":[[2013,9,19]],"date-time":"2013-09-19T03:31:48Z","timestamp":1379561508000},"page":"3988-3992","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":74,"title":["Evaluation of the Nanosphere Verigene Gram-Positive Blood Culture Assay with the VersaTREK Blood Culture System and Assessment of Possible Impact on Selected Patients"],"prefix":"10.1128","volume":"51","author":[{"given":"Stacy G.","family":"Beal","sequence":"first","affiliation":[{"name":"Department of Pathology, UT Southwestern Medical Center, Dallas, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jane","family":"Ciurca","sequence":"additional","affiliation":[{"name":"St. Paul University Hospital, Dallas, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Geremy","family":"Smith","sequence":"additional","affiliation":[{"name":"St. Paul University Hospital, Dallas, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey","family":"John","sequence":"additional","affiliation":[{"name":"St. Paul University Hospital, Dallas, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesca","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Pathology, UT Southwestern Medical Center, Dallas, Texas, USA"},{"name":"Department of Internal Medicine, Division of Infectious Diseases, UT Southwestern Medical Center, Dallas, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher D.","family":"Doern","sequence":"additional","affiliation":[{"name":"Department of Pathology, UT Southwestern Medical Center, Dallas, Texas, USA"},{"name":"Department of Pediatrics, UT Southwestern Medical Center, Dallas, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rita M.","family":"Gander","sequence":"additional","affiliation":[{"name":"Department of Pathology, UT Southwestern Medical Center, Dallas, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa022139"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1097\/00003246-200107000-00002"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1097\/CCM.0b013e3181cc4824"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.06169-11"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1086\/424520"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1086\/656623"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02150-09"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijmm.2013.03.002"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.2147\/TCRM.S2838"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00043-09"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02982-12"},{"key":"e_1_3_2_13_2","volume-title":"Verigene Gram-positive blood culture nucleic acid test (BC-GP) package insert","author":"Nanosphere, Inc","year":"2012","unstructured":"Nanosphere, Inc. 2012. Verigene Gram-positive blood culture nucleic acid test (BC-GP) package insert. Nanosphere Inc., Northbrook, IL."},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1007\/s40291-013-0021-z"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02680-12"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00831-13"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00801-08"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.01889-13","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.01889-13","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T11:28:09Z","timestamp":1646479689000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.01889-13"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,12]]},"references-count":16,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2013,12]]}},"alternative-id":["10.1128\/JCM.01889-13"],"URL":"https:\/\/doi.org\/10.1128\/jcm.01889-13","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,12]]},"assertion":[{"value":"2013-07-16","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-09-12","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-11-15","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}