{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T02:16:45Z","timestamp":1791166605501,"version":"4.1.0"},"reference-count":30,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2011,3,1]],"date-time":"2011-03-01T00:00:00Z","timestamp":1298937600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2011,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The aim of the present study was to assess the diagnostic efficacy of a combination of two quantitative\n            <jats:named-content content-type=\"genus-species\">Aspergillus<\/jats:named-content>\n            PCR assays, targeting a mitochondrial and a ribosomal target, in patients with risk factors for invasive aspergillosis (IA) and positive galactomannan (GM) antigen. Forty-four patients with hematological malignancies and risk factors for IA according to revised European Organization for Research on Treatment of Cancer and the Mycoses Study Group criteria (EORTC\/MSG) criteria and presenting at least two sequential GM-positive sera were included in the study. Mitochondrial PCR was carried out prospectively on all GM-positive serum samples. Ribosomal PCR was carried out retrospectively on frozen stored sera. The sensitivities of mitochondrial and ribosomal PCRs were 58% and 50%, respectively. The diagnostic test performance was improved by using a combination of both PCR assays and by considering a patient PCR positive when at least two positive results were obtained. The sensitivity, specificity, and positive and negative likelihood ratios were 65%, 94%, and 11.8 and 0.37, respectively. A significant association between fatal outcome at 90 days and positive results of ribosomal PCR assays was observed (adjusted hazard ratio = 8.2; 95% confidence interval [CI] = 1.0 to 65.8;\n            <jats:italic>P<\/jats:italic>\n            = 0.048). Our results showed that the combination of two PCR assays targeting mitochondrial and ribosomal\n            <jats:named-content content-type=\"genus-species\">Aspergillus<\/jats:named-content>\n            DNA improves the sensitivity of PCR in the diagnosis of IA in hematological patients with risk factors and positive GM results. This study also confirms that a positive PCR result is associated with a poor prognosis in these patients and should lead to specific antifungal therapy being introduced immediately.\n          <\/jats:p>","DOI":"10.1128\/jcm.01904-10","type":"journal-article","created":{"date-parts":[[2011,1,13]],"date-time":"2011-01-13T03:21:49Z","timestamp":1294888909000},"page":"1058-1063","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":32,"title":["Ribosomal and Mitochondrial DNA Target for Real-Time PCR Diagnosis of Invasive Aspergillosis"],"prefix":"10.1128","volume":"49","author":[{"given":"L.","family":"Millon","sequence":"first","affiliation":[{"name":"CNRS-Univ Franche-Comte, UMR 6249 Chrono-Environnement, 25030 Besan\u00e7on, France"},{"name":"Centre Hospitalier Universitaire, Parasitologie-Mycologie, 25030 Besan\u00e7on, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.","family":"Grenouillet","sequence":"additional","affiliation":[{"name":"CNRS-Univ Franche-Comte, UMR 6249 Chrono-Environnement, 25030 Besan\u00e7on, France"},{"name":"Centre Hospitalier Universitaire, Parasitologie-Mycologie, 25030 Besan\u00e7on, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.","family":"Legrand","sequence":"additional","affiliation":[{"name":"Centre Hospitalier Universitaire, H\u00e9matologie Clinique, 25030 Besan\u00e7on, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Loewert","sequence":"additional","affiliation":[{"name":"Centre Hospitalier Universitaire, Parasitologie-Mycologie, 25030 Besan\u00e7on, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. P.","family":"Bellanger","sequence":"additional","affiliation":[{"name":"CNRS-Univ Franche-Comte, UMR 6249 Chrono-Environnement, 25030 Besan\u00e7on, France"},{"name":"Centre Hospitalier Universitaire, Parasitologie-Mycologie, 25030 Besan\u00e7on, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"Gbaguidi-Haore","sequence":"additional","affiliation":[{"name":"CNRS-Univ Franche-Comte, UMR 6249 Chrono-Environnement, 25030 Besan\u00e7on, France"},{"name":"Centre Hospitalier Universitaire, Hygi\u00e8ne Hospitali\u00e8re, 25030 Besan\u00e7on, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.","family":"Scherer","sequence":"additional","affiliation":[{"name":"CNRS-Univ Franche-Comte, UMR 6249 Chrono-Environnement, 25030 Besan\u00e7on, France"},{"name":"Centre Hospitalier Universitaire, Parasitologie-Mycologie, 25030 Besan\u00e7on, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"Henon","sequence":"additional","affiliation":[{"name":"Centre Hospitalier Universitaire, Pharmacie Centrale, 25030 Besan\u00e7on, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Rohrlich","sequence":"additional","affiliation":[{"name":"Centre Hospitalier Universitaire, H\u00e9matologie Clinique, 25030 Besan\u00e7on, France"},{"name":"Inserm, U645, Besan\u00e7on, Univ Franche-Comte, IFR 133, F-25020 France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.","family":"Deconinck","sequence":"additional","affiliation":[{"name":"Centre Hospitalier Universitaire, H\u00e9matologie Clinique, 25030 Besan\u00e7on, France"},{"name":"Inserm, U645, Besan\u00e7on, Univ Franche-Comte, IFR 133, F-25020 France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1086\/323335"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.47212-0"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.0007-1048.2002.03337.x"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0507.2008.01512.x"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.2.844-846.2004"},{"key":"e_1_3_2_7_2","first-page":"986","article-title":"Invasive fungal infections in patients with hematologic malignancies in a tertiary care cancer center: an autopsy study over a 15-year period (1989\u20132003)","volume":"91","author":"Chamilos G.","year":"2006","unstructured":"ChamilosG.. 2006. Invasive fungal infections in patients with hematologic malignancies in a tertiary care cancer center: an autopsy study over a 15-year period (1989\u20132003). Haematologica 91:986\u2013989.","journal-title":"Haematologica"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.6.2224-2227.2002"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-7012(01)00212-3"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.329.7458.168"},{"key":"e_1_3_2_11_2","doi-asserted-by":"crossref","first-page":"1813","DOI":"10.1086\/588660","article-title":"Revised definitions of invasive fungal disease from the European Organization for Research and Treatment of Cancer\/Invasive Fungal Infections Cooperative Group and the National Institute of Allergy and Infectious Diseases Mycoses Study Group (EORTC\/MSG) Consensus Group","volume":"46","author":"De Pauw B.","year":"2008","unstructured":"De PauwB.. 2008. 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