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The diagnosis is achieved on a clinical, epidemiological, and pathological basis, supported by positive parasitological exams and demonstration of leishmanin delayed-type hypersensitivity. Serological assays are not routinely used in the diagnosis because many are considered to have low sensitivity and the particular\n            <jats:named-content content-type=\"genus-species\">Leishmania<\/jats:named-content>\n            species causing the disease can lead to variable performance. In the present study, we generated recombinant versions of two highly conserved\n            <jats:named-content content-type=\"genus-species\">Leishmania<\/jats:named-content>\n            proteins,\n            <jats:named-content content-type=\"genus-species\">Leishmania<\/jats:named-content>\n            (\n            <jats:named-content content-type=\"genus-species\">Viannia<\/jats:named-content>\n            )\n            <jats:named-content content-type=\"genus-species\">braziliensis<\/jats:named-content>\n            -derived Lb8E and Lb6H, and evaluated both in enzyme-linked immunosorbent assays (ELISA). Recombinant Lb6H (rLb6H) had better performance and reacted with 100.0% of the ATL and 89.4% of the VL samples. These reactions with rLb6H were highly specific (98.5%) when compared against those for samples from healthy control individuals. We then assessed rLb6H against sera from ATL patients infected with different species of\n            <jats:named-content content-type=\"genus-species\">Leishmania<\/jats:named-content>\n            prevalent in Brazil [\n            <jats:named-content content-type=\"genus-species\">Leishmania<\/jats:named-content>\n            (\n            <jats:named-content content-type=\"genus-species\">Leishmania<\/jats:named-content>\n            )\n            <jats:named-content content-type=\"genus-species\">amazonensis<\/jats:named-content>\n            ,\n            <jats:italic>L<\/jats:italic>\n            . (\n            <jats:named-content content-type=\"genus-species\">Viannia<\/jats:named-content>\n            )\n            <jats:named-content content-type=\"genus-species\">braziliensis<\/jats:named-content>\n            , and\n            <jats:italic>L<\/jats:italic>\n            . (\n            <jats:italic>V<\/jats:italic>\n            .)\n            <jats:named-content content-type=\"genus-species\">guyanensis<\/jats:named-content>\n            ] and samples from patients with other infectious diseases. In analyses of 500 sera, ELISA using rLb6H detected all 219 ATL samples (sensitivity of 100.0%) with an overall specificity of 93.9% (considering healthy individuals and other infectious diseases patients). Only a minority of samples from Chagas disease patients possessed antibodies against rLb6H, and all of these responses were low (with a highest reactivity index of 2.2). Taken together, our data support further evaluation of rLb6H and the potential for its routine use in the serological diagnosis of ATL.\n          <\/jats:p>","DOI":"10.1128\/jcm.01904-16","type":"journal-article","created":{"date-parts":[[2016,12,8]],"date-time":"2016-12-08T03:00:43Z","timestamp":1481166043000},"page":"495-503","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":30,"title":["Use of Recombinant Antigens for Sensitive Serodiagnosis of American Tegumentary Leishmaniasis Caused by Different Leishmania Species"],"prefix":"10.1128","volume":"55","author":[{"given":"Camila Massae","family":"Sato","sequence":"first","affiliation":[{"name":"Instituto de Medicina Tropical de S\u00e3o Paulo, Universidade de S\u00e3o Paulo, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Carmen Arroyo","family":"Sanchez","sequence":"additional","affiliation":[{"name":"Instituto de Medicina Tropical de S\u00e3o Paulo, Universidade de S\u00e3o Paulo, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beatriz Julieta","family":"Celeste","sequence":"additional","affiliation":[{"name":"Instituto de Medicina Tropical de S\u00e3o Paulo, Universidade de S\u00e3o Paulo, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Malcolm S.","family":"Duthie","sequence":"additional","affiliation":[{"name":"Infectious Disease Research Institute, Seattle, Washington, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey","family":"Guderian","sequence":"additional","affiliation":[{"name":"Infectious Disease Research Institute, Seattle, Washington, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven G.","family":"Reed","sequence":"additional","affiliation":[{"name":"Infectious Disease Research Institute, Seattle, Washington, 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