{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T00:11:06Z","timestamp":1789863066501,"version":"4.0.1"},"reference-count":16,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2011,5,1]],"date-time":"2011-05-01T00:00:00Z","timestamp":1304208000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2011,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Tuberculosis (TB) is a disease of major public health concern worldwide, especially in developing countries. In addition, the human immunodeficiency virus (HIV) epidemic has increased the incidence of infection with nontuberculous mycobacteria (NTM). Rapid, accurate, and simple methods for differentiation of\n            <jats:named-content content-type=\"genus-species\">Mycobacterium tuberculosis<\/jats:named-content>\n            complex (MTBC) isolates from NTM is greatly needed for successful control of the TB epidemic. This study was done to evaluate the clinical performance of the BD MGIT TBc identification test (TBc ID) for rapid identification of MTBC in samples from broth cultures. A total of 229 Ziehl-Neelsen (ZN) stain-positive MGIT cultures were tested using the TBc ID test, and the results were compared with those of the AccuProbe MTBC identification test (GenProbe, San Diego, CA). The agreement between the TBc ID test and the AccuProbe assay was 96% (kappa = 0.92; confidence interval [CI] = 0.869 to 0.971). The sensitivity, specificity, and negative and positive predictive values of the TBc ID test compared to the AccuProbe assay were 100%, 92.4%, 100%, and 92.2%, respectively. After additional molecular testing, the agreement between the two methods increased to 97.8% (kappa = 0.96; CI = 0.917 to 0.994), and the specificity and positive predictive value increased to 95.6% and 95.7%, respectively. The TBc ID test is a simple, sensitive, and specific test for identification of MTBC in samples from acid-fast bacillus (AFB) smear-positive cultures. The TBc ID test could be a good alternative to the AccuProbe test in TB diagnostic laboratories.\n          <\/jats:p>","DOI":"10.1128\/jcm.01906-10","type":"journal-article","created":{"date-parts":[[2011,3,17]],"date-time":"2011-03-17T00:54:24Z","timestamp":1300323264000},"page":"1939-1942","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":21,"title":["Evaluation of TBc Identification Immunochromatographic Assay for Rapid Identification of Mycobacterium tuberculosis Complex in Samples from Broth Cultures"],"prefix":"10.1128","volume":"49","author":[{"given":"Halima M.","family":"Said","sequence":"first","affiliation":[{"name":"Department of Medical Microbiology, Faculty of Health Science, University of Pretoria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nazir","family":"Ismail","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Faculty of Health Science, University of Pretoria"},{"name":"National Health Laboratory Service, Tshwane Academic Division, Pretoria, South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ayman","family":"Osman","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Faculty of Health Science, University of Pretoria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chrisna","family":"Velsman","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Faculty of Health Science, University of Pretoria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. A.","family":"Hoosen","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Faculty of Health Science, University of Pretoria"},{"name":"National Health Laboratory Service, Tshwane Academic Division, Pretoria, South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.37.11.3693-3697.1999"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.26.1.50-53.1988"},{"key":"e_1_3_2_4_2","first-page":"1","article-title":"Use of an immunochromatographic kit for the rapid detection of Mycobacterium tuberculosis from broth cultures","volume":"13","author":"Ismail N. A.","year":"2009","unstructured":"IsmailN. A. BabaK. PomboD. HoosenA. A.. 2009. Use of an immunochromatographic kit for the rapid detection of Mycobacterium tuberculosis from broth cultures. Int. J. Tuberc. Lung Dis. 13:1\u20133.","journal-title":"Int. J. Tuberc. Lung Dis."},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.5858\/2008-132-1333-NMI"},{"key":"e_1_3_2_6_2","first-page":"28","article-title":"Rapid diagnosis of mycobacterial diseases, and their implications on clinical management","volume":"53","author":"Johansen I. S.","year":"2006","unstructured":"JohansenI. S. 2006. Rapid diagnosis of mycobacterial diseases, and their implications on clinical management. Dan. Med. Bull. 53:28\u201345.","journal-title":"Dan. Med. Bull."},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.26.10.2120-2123.1988"},{"key":"e_1_3_2_8_2","first-page":"541","article-title":"MPB 64 mycobacterial antigen: a new skin test reagent through patch method for rapid diagnosis of active tuberculosis","volume":"2","author":"Nakamura R. M.","year":"1998","unstructured":"NakamuraR. M. . 1998. MPB 64 mycobacterial antigen: a new skin test reagent through patch method for rapid diagnosis of active tuberculosis. Int. J. Tuberc. Lung. Dis. 2:541\u2013546.","journal-title":"Int. J. Tuberc. Lung. Dis."},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2008.05.023"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.62.5.2058-2064.1994"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01253-08"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(06)70549-7"},{"key":"e_1_3_2_13_2","first-page":"59","article-title":"Rapid identification of Mycobacterium tuberculosis by an immunochromatography assay using anti-MPB64 monoclonal antibodies","volume":"1","author":"Tomiyama T.","year":"1997","unstructured":"TomiyamaT. MastuoK. AbeC.. 1997. Rapid identification of Mycobacterium tuberculosis by an immunochromatography assay using anti-MPB64 monoclonal antibodies. Int. J. Tuberc. Lung Dis. 1:59.","journal-title":"Int. J. Tuberc. Lung Dis."},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2007.06.010"},{"key":"e_1_3_2_15_2","first-page":"102","volume-title":"Abstr. 30th Annu. Cong. Eur. Soc. Microbacteriol.","author":"Warns M.","year":"2009","unstructured":"WarnsM. . 2009. Evaluation of the MGIT TBc ID test vs. two commercially available rapid immunoassays for M. tuberculosis complex organism detection from liquid and solid culture, p. 102. Abstr. 30th Annu. Cong. Eur. Soc. Microbacteriol., Porto, Portugal."},{"key":"e_1_3_2_16_2","unstructured":"World Health Organization. 2007. Global tuberculosis control. WHO Geneva Switzerland. http:\/\/www.who.int\/tb\/publications\/global_report\/2007\/key_findings\/en\/. Accessed 12 October 2009."},{"key":"e_1_3_2_17_2","unstructured":"World Health Organization. 2009. Global tuberculosis control: epidemiology strategy financing. WHO Geneva Switzerland. http:\/\/www.who.int\/tb\/publications\/global_report\/en\/. Accessed 12 October 2009."}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.01906-10","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.01906-10","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T12:56:24Z","timestamp":1646484984000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.01906-10"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,5]]},"references-count":16,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2011,5]]}},"alternative-id":["10.1128\/JCM.01906-10"],"URL":"https:\/\/doi.org\/10.1128\/jcm.01906-10","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,5]]},"assertion":[{"value":"2010-09-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2011-03-03","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2011-04-27","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}