{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T02:11:03Z","timestamp":1790475063957,"version":"4.1.0"},"reference-count":30,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2014,11,1]],"date-time":"2014-11-01T00:00:00Z","timestamp":1414800000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2014,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The molecular diagnosis of toxoplasmosis essentially relies upon laboratory-developed methods and suffers from lack of standardization, hence the large diversity of performances between laboratories. Moreover, quantifications of parasitic loads differ among centers, a fact which prevents the possible prediction of the severity of this disease as a function of parasitic loads. The objectives of this multicentric study performed in eight proficient laboratories of the Molecular Biology Pole of the French National Reference Center for Toxoplasmosis (NRC-T) were (i) to assess the suitability of a lyophilized preparation of\n            <jats:named-content content-type=\"genus-species\">Toxoplasma gondii<\/jats:named-content>\n            as a common standard for use in this PCR-based molecular diagnosis and (ii) to make this standard available to the community. High-quality written procedures were used for the production and qualification of this standard. Three independent batches of this standard, containing concentrations ranging from 10\n            <jats:sup>4<\/jats:sup>\n            to 0.01\n            <jats:named-content content-type=\"genus-species\">T. gondii<\/jats:named-content>\n            genome equivalents per PCR, were first assessed: the linear dynamic range was \u22656 log, the intra-assay coefficients of variation (CV) from a sample containing 10\n            <jats:named-content content-type=\"genus-species\">T. gondii<\/jats:named-content>\n            organisms per PCR were 0.3% to 0.42%, and the interassay CV over a 2-week period was 0.76% to 1.47%. A further assessment in eight diagnostic centers showed that the standard is stable, robust, and reliable. These lyophilized standards can easily be produced at a larger scale when needed and can be made widely available at the national level. To our knowledge, this is the first quality control assessment of a common standard which is usable both for self-evaluation in laboratories and for accurate quantification of parasitic loads in\n            <jats:named-content content-type=\"genus-species\">T. gondii<\/jats:named-content>\n            prenatal infections.\n          <\/jats:p>","DOI":"10.1128\/jcm.01906-14","type":"journal-article","created":{"date-parts":[[2014,9,4]],"date-time":"2014-09-04T17:44:53Z","timestamp":1409852693000},"page":"3952-3959","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":29,"title":["Characterization and Multicentric Validation of a Common Standard for Toxoplasma gondii Detection Using Nucleic Acid Amplification Assays"],"prefix":"10.1128","volume":"52","author":[{"given":"Emmanuelle","family":"Varlet-Marie","sequence":"first","affiliation":[{"name":"CHU (University Hospital Centre) of Montpellier\/University Montpellier I, Laboratoire de Parasitologie-Mycologie, Montpellier, France"},{"name":"P\u00f4le Biologie Mol\u00e9culaire du Centre National de R\u00e9f\u00e9rence de la Toxoplasmose, Montpellier, France"},{"name":"University Montpellier I, Faculty of Pharmacy, Laboratoire de Biophysique &amp; Bioanalyse, Montpellier, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yvon","family":"Sterkers","sequence":"additional","affiliation":[{"name":"CHU (University Hospital Centre) of Montpellier\/University Montpellier I, Laboratoire de Parasitologie-Mycologie, Montpellier, France"},{"name":"P\u00f4le Biologie Mol\u00e9culaire du Centre National de R\u00e9f\u00e9rence de la Toxoplasmose, Montpellier, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie-Pierre","family":"Brenier-Pinchart","sequence":"additional","affiliation":[{"name":"P\u00f4le Biologie Mol\u00e9culaire du Centre National de R\u00e9f\u00e9rence de la Toxoplasmose, Montpellier, France"},{"name":"CHU A. Michallon\/University Joseph Fourier Grenoble I, Laboratoire de Parasitologie-Mycologie, D\u00e9partement des Agents Infectieux, Grenoble, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sophie","family":"Cassaing","sequence":"additional","affiliation":[{"name":"P\u00f4le Biologie Mol\u00e9culaire du Centre National de R\u00e9f\u00e9rence de la Toxoplasmose, Montpellier, France"},{"name":"CHU of Toulouse, Service de Parasitologie-Mycologie, Toulouse, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fr\u00e9d\u00e9ric","family":"Dalle","sequence":"additional","affiliation":[{"name":"P\u00f4le Biologie Mol\u00e9culaire du Centre National de R\u00e9f\u00e9rence de la Toxoplasmose, Montpellier, France"},{"name":"CHU of Dijon, Laboratoire de Parasitologie-Mycologie, Faculty of Medicine, Universit\u00e9 de Bourgogne, UMR 1347 Aggro\u00e9cologie, Dijon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurence","family":"Delhaes","sequence":"additional","affiliation":[{"name":"P\u00f4le Biologie Mol\u00e9culaire du Centre National de R\u00e9f\u00e9rence de la Toxoplasmose, Montpellier, France"},{"name":"CHU of Lille\/Universit\u00e9 Nord de France (UDSL), Service de Parasitologie-Mycologie, Faculty of Medicine, Institut Pasteur de Lille, Lille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Denis","family":"Filisetti","sequence":"additional","affiliation":[{"name":"P\u00f4le Biologie Mol\u00e9culaire du Centre National de R\u00e9f\u00e9rence de la Toxoplasmose, Montpellier, France"},{"name":"H\u00f4pitaux Universitaires de Strasbourg\/University of Strasbourg, Laboratoire de Parasitologie et Mycologie M\u00e9dicales, Strasbourg, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Herv\u00e9","family":"Pelloux","sequence":"additional","affiliation":[{"name":"P\u00f4le Biologie Mol\u00e9culaire du Centre National de R\u00e9f\u00e9rence de la Toxoplasmose, Montpellier, France"},{"name":"CHU A. Michallon\/University Joseph Fourier Grenoble I, Laboratoire de Parasitologie-Mycologie, D\u00e9partement des Agents Infectieux, Grenoble, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F\u00e9riel","family":"Touafek","sequence":"additional","affiliation":[{"name":"P\u00f4le Biologie Mol\u00e9culaire du Centre National de R\u00e9f\u00e9rence de la Toxoplasmose, Montpellier, France"},{"name":"AP-HP, Groupe Hospitalier Piti\u00e9-Salp\u00eatri\u00e8re, Laboratoire de Parasitologie-Mycologie, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H\u00e9l\u00e8ne","family":"Yera","sequence":"additional","affiliation":[{"name":"P\u00f4le Biologie Mol\u00e9culaire du Centre National de R\u00e9f\u00e9rence de la Toxoplasmose, Montpellier, France"},{"name":"University Paris Descartes, AP-HP, Groupe Hospitalier Cochin, Laboratoire de Parasitologie-Mycologie, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick","family":"Bastien","sequence":"additional","affiliation":[{"name":"CHU (University Hospital Centre) of Montpellier\/University Montpellier I, Laboratoire de Parasitologie-Mycologie, Montpellier, France"},{"name":"P\u00f4le Biologie Mol\u00e9culaire du Centre National de R\u00e9f\u00e9rence de la Toxoplasmose, Montpellier, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1086\/590149"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0035-9203(02)90078-7"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00918-12"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02258-07"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0223(200102)21:2<85::AID-PD18>3.0.CO;2-1"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2003.09.039"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1998.tb13151.x"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2010.03101.x"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cca.2006.06.016"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02500-09"},{"key":"e_1_3_2_12_2","first-page":"43","article-title":"Screening programme for congenital toxoplasmosis in France","volume":"84","author":"Thulliez P","year":"1992","unstructured":"ThulliezP. 1992. 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