{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T04:24:23Z","timestamp":1792988663957,"version":"4.5.0"},"reference-count":25,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2010,5,1]],"date-time":"2010-05-01T00:00:00Z","timestamp":1272672000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2010,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            In earlier studies we used molecular methods to identify the major bacterial consortia associated with advanced dentin caries. These consortia are dominated by bacteria from the families\n            <jats:italic>Lactobacillaceae<\/jats:italic>\n            ,\n            <jats:italic>Streptococcaceae<\/jats:italic>\n            ,\n            <jats:italic>Veillonellaceae<\/jats:italic>\n            (formerly\n            <jats:italic>Acidaminococcaceae<\/jats:italic>\n            ),\n            <jats:italic>Eubacteriaceae<\/jats:italic>\n            , and\n            <jats:italic>Lachnospiraceae<\/jats:italic>\n            from the phylum\n            <jats:italic>Firmicutes<\/jats:italic>\n            ;\n            <jats:italic>Coriobacteriaceae<\/jats:italic>\n            ,\n            <jats:italic>Bifidobacteriaceae<\/jats:italic>\n            , and\n            <jats:italic>Propionibacteriaceae<\/jats:italic>\n            from the phylum\n            <jats:italic>Actinobacteria<\/jats:italic>\n            ; and\n            <jats:italic>Prevotellaceae<\/jats:italic>\n            from the phylum\n            <jats:italic>Bacteroidetes<\/jats:italic>\n            , as well as fusobacteria. The phases of infection of vital pulp tissue by dentin microorganisms remain obscure. In the present study, fluorescence\n            <jats:italic>in situ<\/jats:italic>\n            hybridization was performed on sections of tissue embedded in resin. Probes for 16S rRNA corresponding to the major taxa of bacteria in carious dentin were used to provide information on the characteristics of pulp infection. Lactobacilli were prominent in 7 of 8 pulps determined to be at a limited stage of infection. Established infection (6 pulps) showed a more complex profile, with lactobacilli persisting in all of the lesions and with invasion of the necrotic regions of tissue by\n            <jats:italic>Bacteroidetes<\/jats:italic>\n            , fusobacteria,\n            <jats:italic>Lachnospiraceae<\/jats:italic>\n            , and\n            <jats:italic>Coriobacteriaceae<\/jats:italic>\n            in particular. Advanced infections (7 pulps) were characterized by mixed anaerobic species, with a strong representation by\n            <jats:italic>Coriobacteriaceae<\/jats:italic>\n            and\n            <jats:italic>Lachnospiraceae<\/jats:italic>\n            . Lactobacilli were not represented at this stage. Typically, groups of organisms were spatially isolated within the pulp tissue. Analysis indicated that lactobacilli could invade vital pulp tissue to achieve a very high biomass that was not associated with a detectable local inflammatory infiltrate. The findings establish that invasion of the dental pulp can be associated with a pronounced selection from the complex microbial populations within carious dentin, suggesting specific pathogenicity.\n          <\/jats:p>","DOI":"10.1128\/jcm.01912-09","type":"journal-article","created":{"date-parts":[[2010,3,4]],"date-time":"2010-03-04T02:54:02Z","timestamp":1267671242000},"page":"1732-1740","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":30,"title":["Lactobacilli Are Prominent in the Initial Stages of Polymicrobial Infection of Dental Pulp"],"prefix":"10.1128","volume":"48","author":[{"given":"Mangala A.","family":"Nadkarni","sequence":"first","affiliation":[{"name":"Institute of Dental Research, Westmead Millennium Institute and Westmead Centre for Oral Health, Westmead, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mary R.","family":"Simonian","sequence":"additional","affiliation":[{"name":"Institute of Dental Research, Westmead Millennium Institute and Westmead Centre for Oral Health, Westmead, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Derek W. S.","family":"Harty","sequence":"additional","affiliation":[{"name":"Institute of Dental Research, Westmead Millennium Institute and Westmead Centre for Oral Health, Westmead, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hans","family":"Zoellner","sequence":"additional","affiliation":[{"name":"Department of Oral Pathology, University of Sydney, Sydney, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicholas A.","family":"Jacques","sequence":"additional","affiliation":[{"name":"Institute of Dental Research, Westmead Millennium Institute and Westmead Centre for Oral Health, Westmead, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neil","family":"Hunter","sequence":"additional","affiliation":[{"name":"Institute of Dental Research, Westmead Millennium Institute and Westmead Centre for Oral Health, Westmead, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.172.2.762-770.1990"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.7.3128-3136.2004"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-33-2-426"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.2.843-849.2005"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.3.1270-1273.2003"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/0168-1605(94)90117-1"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.177.12.8785"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2591.1992.tb00941.x"},{"key":"e_1_3_2_10_2","first-page":"29","volume":"179","year":"2002","unstructured":"Hugenholtz, P., G. 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