{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T02:11:04Z","timestamp":1790475064937,"version":"4.1.0"},"reference-count":29,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2015,1,1]],"date-time":"2015-01-01T00:00:00Z","timestamp":1420070400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2015,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The detection of\n            <jats:named-content content-type=\"genus-species\">Toxoplasma gondii<\/jats:named-content>\n            in amniotic fluid is an essential tool for the prenatal diagnosis of congenital toxoplasmosis and is currently essentially based on the use of PCR. Although some consensus is emerging, this molecular diagnosis suffers from a lack of standardization and an extreme diversity of laboratory-developed methods. Commercial kits for the detection of\n            <jats:named-content content-type=\"genus-species\">T. gondii<\/jats:named-content>\n            by PCR were recently developed and offer certain advantages; however, they must be assessed in comparison with optimized reference PCR assays. The present multicentric study aimed to compare the performances of the Bio-Evolution\n            <jats:named-content content-type=\"genus-species\">T. gondii<\/jats:named-content>\n            detection kit and laboratory-developed PCR assays set up in eight proficient centers in France. The study compared 157 amniotic fluid samples and found concordances of 99% and 100% using 76\n            <jats:named-content content-type=\"genus-species\">T. gondii<\/jats:named-content>\n            -infected samples and 81 uninfected samples, respectively. Moreover, taking into account the classification of the European Research Network on Congenital Toxoplasmosis, the overall diagnostic sensitivity of all assays was identical and calculated to be 86% (54\/63); specificity was 100% for all assays. Finally, the relative quantification results were in good agreement between the kit and the laboratory-developed assays. The good performances of this commercial kit are probably in part linked to the use of a number of good practices: detection in multiplicate, amplification of the repetitive DNA target rep529, and the use of an internal control for the detection of PCR inhibitors. The only drawbacks noted at the time of the study were the absence of uracil-\n            <jats:italic>N<\/jats:italic>\n            -glycosylase and small defects in the reliability of the production of different reagents.\n          <\/jats:p>","DOI":"10.1128\/jcm.01913-14","type":"journal-article","created":{"date-parts":[[2014,10,23]],"date-time":"2014-10-23T02:07:39Z","timestamp":1414030059000},"page":"29-34","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":27,"title":["Multicentric Comparative Assessment of the Bio-Evolution Toxoplasma gondii Detection Kit with Eight Laboratory-Developed PCR Assays for Molecular Diagnosis of Congenital Toxoplasmosis"],"prefix":"10.1128","volume":"53","author":[{"given":"Denis","family":"Filisetti","sequence":"first","affiliation":[{"name":"Institut de Parasitologie et de Pathologie Tropicale, Universit\u00e9 de Strasbourg, Laboratoire de Parasitologie et Mycologie M\u00e9dicale, H\u00f4pitaux Universitaires de Strasbourg, Strasbourg, France"},{"name":"P\u00f4le Biologie Mol\u00e9culaire du Centre National de R\u00e9f\u00e9rence de la Toxoplasmose, Montpellier, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yvon","family":"Sterkers","sequence":"additional","affiliation":[{"name":"Centre Hospitalier Universitaire (CHU) of Montpellier\/University Montpellier I, Laboratoire de Parasitologie-Mycologie, UMR CNRS 5290\/IRD 224\/UM, Montpellier, France"},{"name":"P\u00f4le Biologie Mol\u00e9culaire du Centre National de R\u00e9f\u00e9rence de la Toxoplasmose, Montpellier, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie-Pierre","family":"Brenier-Pinchart","sequence":"additional","affiliation":[{"name":"P\u00f4le Biologie Mol\u00e9culaire du Centre National de R\u00e9f\u00e9rence de la Toxoplasmose, Montpellier, France"},{"name":"CHU A. Michallon\/University Joseph Fourier Grenoble I, Laboratoire de Parasitologie-Mycologie, D\u00e9partement des Agents Infectieux, Grenoble, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sophie","family":"Cassaing","sequence":"additional","affiliation":[{"name":"P\u00f4le Biologie Mol\u00e9culaire du Centre National de R\u00e9f\u00e9rence de la Toxoplasmose, Montpellier, France"},{"name":"CHU of Toulouse, Service de Parasitologie-Mycologie, Toulouse, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fr\u00e9d\u00e9ric","family":"Dalle","sequence":"additional","affiliation":[{"name":"P\u00f4le Biologie Mol\u00e9culaire du Centre National de R\u00e9f\u00e9rence de la Toxoplasmose, Montpellier, France"},{"name":"CHU of Dijon, Laboratoire de Parasitologie-Mycologie, Faculty of Medicine, Dijon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurence","family":"Delhaes","sequence":"additional","affiliation":[{"name":"P\u00f4le Biologie Mol\u00e9culaire du Centre National de R\u00e9f\u00e9rence de la Toxoplasmose, Montpellier, France"},{"name":"CHU of Lille\/University Nord de France (UDSL), Service de Parasitologie-Mycologie, Faculty of Medicine, Institut Pasteur de Lille, Lille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Herv\u00e9","family":"Pelloux","sequence":"additional","affiliation":[{"name":"P\u00f4le Biologie Mol\u00e9culaire du Centre National de R\u00e9f\u00e9rence de la Toxoplasmose, Montpellier, France"},{"name":"CHU A. Michallon\/University Joseph Fourier Grenoble I, Laboratoire de Parasitologie-Mycologie, D\u00e9partement des Agents Infectieux, Grenoble, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F\u00e9riel","family":"Touafek","sequence":"additional","affiliation":[{"name":"P\u00f4le Biologie Mol\u00e9culaire du Centre National de R\u00e9f\u00e9rence de la Toxoplasmose, Montpellier, France"},{"name":"AP-HP, Groupe Hospitalier Piti\u00e9-Salp\u00eatri\u00e8re, Laboratoire de Parasitologie-Mycologie, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emmanuelle","family":"Varlet-Marie","sequence":"additional","affiliation":[{"name":"Centre Hospitalier Universitaire (CHU) of Montpellier\/University Montpellier I, Laboratoire de Parasitologie-Mycologie, UMR CNRS 5290\/IRD 224\/UM, Montpellier, France"},{"name":"P\u00f4le Biologie Mol\u00e9culaire du Centre National de R\u00e9f\u00e9rence de la Toxoplasmose, Montpellier, France"},{"name":"University Montpellier 1, Faculty of Pharmacy, Laboratoire de Biophysique &amp; Bioanalyse, Montpellier, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H\u00e9l\u00e8ne","family":"Yera","sequence":"additional","affiliation":[{"name":"P\u00f4le Biologie Mol\u00e9culaire du Centre National de R\u00e9f\u00e9rence de la Toxoplasmose, Montpellier, France"},{"name":"Universit\u00e9 Paris-Descartes, AP-HP, Groupe Hospitalier Cochin, Laboratoire de Parasitologie-Mycologie, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ermano","family":"Candolfi","sequence":"additional","affiliation":[{"name":"Institut de Parasitologie et de Pathologie Tropicale, Universit\u00e9 de Strasbourg, Laboratoire de Parasitologie et Mycologie M\u00e9dicale, H\u00f4pitaux Universitaires de Strasbourg, Strasbourg, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick","family":"Bastien","sequence":"additional","affiliation":[{"name":"Centre Hospitalier Universitaire (CHU) of Montpellier\/University Montpellier I, Laboratoire de Parasitologie-Mycologie, UMR CNRS 5290\/IRD 224\/UM, Montpellier, France"},{"name":"P\u00f4le Biologie Mol\u00e9culaire du Centre National de R\u00e9f\u00e9rence de la Toxoplasmose, Montpellier, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00918-12"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/pd.1970140309"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-0528.1996.tb09805.x"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0035-9203(02)90078-7"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02258-07"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1590\/S0074-02762009000200038"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1097\/AOG.0b013e3181d57b09"},{"key":"e_1_3_3_9_2","first-page":"43","article-title":"Screening programme for congenital toxoplasmosis in France","volume":"84","author":"Thulliez P","year":"1992","unstructured":"Thulliez P. 1992. 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