{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T01:31:40Z","timestamp":1773797500748,"version":"3.50.1"},"reference-count":38,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2006,8,1]],"date-time":"2006-08-01T00:00:00Z","timestamp":1154390400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2006,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            In Brazil,\n            <jats:italic>Salmonella enterica<\/jats:italic>\n            serovar Infantis resistant to various antimicrobials, including cephalosporins, has been identified as an etiological agent of severe gastroenteritis in hospitalized children since 1994. In this study, 35 serovar Infantis strains, isolated from children admitted to four different Rio de Janeiro, Brazil, hospitals between 1996 and 2001, were characterized by pulsed-field gel electrophoresis (PFGE) and antimicrobial susceptibility testing in order to determine their genetic relatedness and antimicrobial resistance profiles. Thirty-four serovar Infantis strains were resistant to at least two antibiotic classes, and all 35 strains were susceptible to fluoroquinolones, cephamycin, and carbapenem. Extended-spectrum beta-lactamase (ESBL) screening by double-disk diffusion indicated that 32 serovar Infantis strains (91.4%) produced beta-lactamases that were inhibited by clavulanic acid. Antimicrobial resistance gene profiles were determined by PCR for a subset of 11 multidrug-resistant serovar Infantis strains, and putative ESBLs were detected by isoelectric focusing. Ten serovar Infantis strains carried\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>TEM<\/jats:sub>\n            ,\n            <jats:italic>catI<\/jats:italic>\n            ,\n            <jats:italic>ant(3<\/jats:italic>\n            \"\n            <jats:italic>)Ia<\/jats:italic>\n            and\/or\n            <jats:italic>ant(3<\/jats:italic>\n            \"\n            <jats:italic>)Ib<\/jats:italic>\n            ,\n            <jats:italic>sulI<\/jats:italic>\n            and\/or\n            <jats:italic>sulII<\/jats:italic>\n            , and\n            <jats:italic>tet<\/jats:italic>\n            (D) genes as well as an integron-associated\n            <jats:italic>aac(6<\/jats:italic>\n            \u2032\n            <jats:italic>)-Iq<\/jats:italic>\n            cassette. Eight strains possessed at least four different beta-lactamases with pI profiles that confirmed the presence of both ESBLs and non-ESBLs. Our PFGE profiles indicated that 33 serovar Infantis strains isolated from Rio de Janeiro hospitals came from the same genetic lineage.\n          <\/jats:p>","DOI":"10.1128\/jcm.01916-05","type":"journal-article","created":{"date-parts":[[2006,8,5]],"date-time":"2006-08-05T01:54:18Z","timestamp":1154742858000},"page":"2767-2772","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":42,"title":["Clonality and Antimicrobial Resistance Gene Profiles of Multidrug- Resistant\n            <i>Salmonella enterica<\/i>\n            Serovar Infantis Isolates from Four Public Hospitals in Rio de Janeiro, Brazil"],"prefix":"10.1128","volume":"44","author":[{"given":"E. L.","family":"Fonseca","sequence":"first","affiliation":[{"name":"Bacteriology Department, Oswaldo Cruz Institute - FIOCRUZ, Rio de Janeiro, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"O. L.","family":"Mykytczuk","sequence":"additional","affiliation":[{"name":"National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada"},{"name":"Department of Medical Microbiology, Faculty of Medicine, University of Manitoba, Winnipeg, Manitoba, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. D.","family":"Asensi","sequence":"additional","affiliation":[{"name":"Bacteriology Department, Oswaldo Cruz Institute - FIOCRUZ, Rio de Janeiro, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E. M. F.","family":"Reis","sequence":"additional","affiliation":[{"name":"Bacteriology Department, Oswaldo Cruz Institute - FIOCRUZ, Rio de Janeiro, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L. R.","family":"Ferraz","sequence":"additional","affiliation":[{"name":"Public Health Laboratory, Brasilia, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F. L.","family":"Paula","sequence":"additional","affiliation":[{"name":"Evandro Chagas Institute, Para, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L. K.","family":"Ng","sequence":"additional","affiliation":[{"name":"National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada"},{"name":"Department of Medical Microbiology, Faculty of Medicine, University of Manitoba, Winnipeg, Manitoba, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D. P.","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"Bacteriology Department, Oswaldo Cruz Institute - FIOCRUZ, Rio de Janeiro, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","unstructured":"Ag\u00eancia Nacional de Vigil\u00e2ncia Sanit\u00e1ria (ANVISA). 2005. Manual de procedimentos b\u00e1sicos em microbiologia cl\u00ednica para o controle de infec\u00e7\u00e3o hospitalar. [Online.] http:\/\/www.hu.usp.br\/arquivos\/Manualccih_2005.pdf\n."},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/282864a0"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(05)80360-2"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/25.17.3389"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1089\/mdr.2000.6.77"},{"key":"e_1_3_2_7_2","first-page":"149","volume":"25","year":"1994","unstructured":"Asensi, M. D., and E. Hofer. 1994. Serovars and multiple drug resistant Salmonella sp. isolated from children in Rio de Janeiro - Brazil. Rev. Microbiol. S\u00e3o Paulo25:149-153.","journal-title":"S\u00e3o Paulo"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.38.5.1172"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.14.4.933-951.2001"},{"key":"e_1_3_2_10_2","first-page":"1400","volume":"143","year":"1980","unstructured":"Bradley, D. E., D. E. Taylor, and D. R. Cohen. 1980. Specification of surface mating systems among conjugative drug resistance plasmids in Escherichia coli K-12. J. Bacteriol.143:1400-1470.","journal-title":"J. Bacteriol."},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.39.6.1211"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0149-2918(02)80062-5"},{"key":"e_1_3_2_13_2","first-page":"120","year":"1972","unstructured":"Costa, G. A., and E. Hofer. 1972. Isolamento e identifica\u00e7\u00e3o de Enterobact\u00e9rias, p. 120. Instituto Oswaldo Cruz - FIOCRUZ, Rio de Janeiro, Brazil.","journal-title":"Isolamento e identifica\u00e7\u00e3o de Enterobact\u00e9rias"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1590\/S0036-46652000000400005"},{"key":"e_1_3_2_15_2","unstructured":"Edelstain M. and L. Stratchounski. 1998. Development of single-strand conformational polymorphism (SSCP) PCR method for discriminatory detection of genes coding for TEM-family \u03b2-lactamases. Program Abstr. 38th Intersci. Conf. Antimicrob. Agents Chemother. poster E-96."},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.37.12.2699"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0891-5520(05)70249-X"},{"key":"e_1_3_2_18_2","unstructured":"Reference deleted."},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.39.1.185"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(86)90315-X"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.24.6.835"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/0147-619X(80)90101-8"},{"key":"e_1_3_2_23_2","unstructured":"Minist\u00e9rio da Sa\u00fade. 1998. Portaria n\u00b0 2.616 de 12 de maio de 1998. Disp\u00f5e sobre a obrigatoriedade da manuten\u00e7\u00e3o do \u201cPrograma Nacional de Controle de Infec\u00e7\u00e3o Hospitalar.\u201d Minist\u00e9rio da Sa\u00fade Brazilia Brazil."},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkh347"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj2720505"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj2520173"},{"key":"e_1_3_2_27_2","unstructured":"Performance standards for antimicrobial susceptibility testing; 30th informational supplement 2004 vol. 24"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.43.12.3018"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1086\/318832"},{"key":"e_1_3_2_30_2","unstructured":"Persing D. H. T. F. Smith F. C. Tenover and T. J. White (ed.). 1993. Diagnostic molecular microbiology: principles and applications. American Society for Microbiology Washington D.C."},{"key":"e_1_3_2_31_2","unstructured":"Poppof M. Y. 2001. Antigenic formulas of the Salmonella serovars. WHO Collaborating Center for Reference and Research for Salmonella Serovars Institut Pasteur Paris France."},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1067\/mpd.2002.127279"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.32.11.1684"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.34.4.642"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.33.9.2233-2239.1995"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.32.9.1416"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.44.10.2777-2783.2000"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.16.3.430-450.2003"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1348-0421.1992.tb02109.x"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.01916-05","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.01916-05","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T05:21:40Z","timestamp":1646457700000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.01916-05"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,8]]},"references-count":38,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2006,8]]}},"alternative-id":["10.1128\/JCM.01916-05"],"URL":"https:\/\/doi.org\/10.1128\/jcm.01916-05","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,8]]},"assertion":[{"value":"2005-09-13","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2006-05-08","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2006-08-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}