{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T20:22:13Z","timestamp":1791663733354,"version":"4.3.4"},"reference-count":27,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2018,4,1]],"date-time":"2018-04-01T00:00:00Z","timestamp":1522540800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2018,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Rapid and accurate laboratory tests are important for the timely diagnosis and treatment of central nervous system infections. The FilmArray meningitis\/encephalitis (ME) panel (BioFire Diagnostics, Salt Lake City, UT) is an FDA-cleared, multiplex molecular panel that allows the detection of 14 pathogens (bacterial [\n            <jats:italic>n<\/jats:italic>\n            = 6], viral [\n            <jats:italic>n<\/jats:italic>\n            = 7], and fungal [\n            <jats:italic>n<\/jats:italic>\n            = 1] pathogens) from cerebrospinal fluid (CSF). In this study, we evaluated the performance characteristics of the FilmArray ME panel using clinical, residual CSF samples (\n            <jats:italic>n<\/jats:italic>\n            = 291) that tested positive by a routine method(s) (e.g., bacterial culture, individual real-time PCR assay) for a pathogen represented on the ME panel. Of note, a subset (\n            <jats:italic>n<\/jats:italic>\n            = 76) of the CSF specimens was collected during the prevaccine era and had been characterized as positive for a bacterial pathogen. The FilmArray ME panel demonstrated an overall percent positive agreement (PPA) of 97.5% (78\/80) for bacterial pathogens, 90.1% (145\/161) for viruses, and 52% (26\/50) for\n            <jats:named-content content-type=\"genus-species\">Cryptococcus<\/jats:named-content>\n            <jats:italic>neoformans\/C. gattii<\/jats:italic>\n            . Despite the low overall agreement (52%) between the ME panel and antigen testing for detection of\n            <jats:italic>C. neoformans\/C. gattii<\/jats:italic>\n            , the percent positive agreement of the FilmArray assay for\n            <jats:italic>C. neoformans\/C. gattii<\/jats:italic>\n            was 92.3% (12\/13) when the results were compared directly to the results of routine fungal smear or culture. The FilmArray ME panel offers a rapid (\u223c60-min), syndrome-based approach for the detection of select meningitis and encephalitis pathogens.\n          <\/jats:p>","DOI":"10.1128\/jcm.01927-17","type":"journal-article","created":{"date-parts":[[2018,2,8]],"date-time":"2018-02-08T14:38:08Z","timestamp":1518100688000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":161,"title":["Evaluation of a Commercial Multiplex Molecular Panel for Diagnosis of Infectious Meningitis and Encephalitis"],"prefix":"10.1128","volume":"56","author":[{"given":"Rachael M.","family":"Liesman","sequence":"first","affiliation":[{"name":"Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, Minnesota, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angela P.","family":"Strasburg","sequence":"additional","affiliation":[{"name":"Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, Minnesota, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angela K.","family":"Heitman","sequence":"additional","affiliation":[{"name":"Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, Minnesota, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elitza S.","family":"Theel","sequence":"additional","affiliation":[{"name":"Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, Minnesota, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6344-4141","authenticated-orcid":false,"given":"Robin","family":"Patel","sequence":"additional","affiliation":[{"name":"Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, Minnesota, USA"},{"name":"Division of Infectious Diseases, Department of Medicine, Mayo Clinic, Rochester, Minnesota, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew J.","family":"Binnicker","sequence":"additional","affiliation":[{"name":"Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, Minnesota, 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