{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T17:14:50Z","timestamp":1792689290337,"version":"4.5.0"},"reference-count":34,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2010,5,1]],"date-time":"2010-05-01T00:00:00Z","timestamp":1272672000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2010,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The GenoType MTBDR\n            <jats:italic>sl<\/jats:italic>\n            test rapidly detects resistance to ethambutol, fluoroquinolones, and second-line aminoglycosides (amikacin and kanamycin) and cyclic peptide (capreomycin) in\n            <jats:italic>Mycobacterium tuberculosis<\/jats:italic>\n            . A set of 41 multidrug-resistant (MDR)\n            <jats:italic>M. tuberculosis<\/jats:italic>\n            strains, 8 extensively drug-resistant (XDR)\n            <jats:italic>M. tuberculosis<\/jats:italic>\n            strains, and 3 non-MDR\n            <jats:italic>M. tuberculosis<\/jats:italic>\n            strains were tested by the MTBDR\n            <jats:italic>sl<\/jats:italic>\n            test and by DNA sequencing of the resistance-determining regions in\n            <jats:italic>gyrA<\/jats:italic>\n            and\n            <jats:italic>gyrB<\/jats:italic>\n            (fluoroquinolones [FQ]),\n            <jats:italic>rpsL<\/jats:italic>\n            (streptomycin),\n            <jats:italic>rrs<\/jats:italic>\n            and\n            <jats:italic>tlyA<\/jats:italic>\n            (aminoglycosides and\/or cyclic peptide), and\n            <jats:italic>embB<\/jats:italic>\n            (ethambutol). The sensitivity and specificity of the MTBDR\n            <jats:italic>sl<\/jats:italic>\n            test were as follows: 87% and 96%, respectively, for fluoroquinolones; 100% for both for amikacin; 77% and 100%, respectively, for kanamycin, 80% and 98%, respectively, for capreomycin; and 57% and 92%, respectively, for ethambutol. Analysis of the discrepant results indicated that three FQ-resistant strains (including one XDR strain) with mutations in\n            <jats:italic>gyrB<\/jats:italic>\n            were missed by the MTBDR\n            <jats:italic>sl<\/jats:italic>\n            test and that one FQ-susceptible strain, identified as resistant by the MTBDR\n            <jats:italic>sl<\/jats:italic>\n            test, had a double mutation (T80A-A90G) in GyrA that did not confer resistance to FQ. Five strains (including two XDR strains) without mutations in\n            <jats:italic>rrs<\/jats:italic>\n            were monoresistant to aminoglycosides or cyclic peptide and were missed by the MTBDR\n            <jats:italic>sl<\/jats:italic>\n            test. Finally, 12\/28 ethambutol-resistant strains had no mutation at codon 306 in\n            <jats:italic>embB<\/jats:italic>\n            , while 2\/24 ethambutol-susceptible strains had such a mutation. In conclusion, the MTBDR\n            <jats:italic>sl<\/jats:italic>\n            test efficiently detects the most common mutations involved in resistance to fluoroquinolones, aminoglycosides\/cyclic peptide, and ethambutol and accurately assesses susceptibility to amikacin. However, due to mutations not included in the test (particularly in\n            <jats:italic>gyrB<\/jats:italic>\n            ) or resistance mechanisms not yet characterized (particularly those related to ethambutol resistance and to monoresistance to aminoglycosides or cyclic peptide), the wild-type results yielded by the MTBDR\n            <jats:italic>sl<\/jats:italic>\n            test should be confirmed by drug susceptibility testing.\n          <\/jats:p>","DOI":"10.1128\/jcm.01947-09","type":"journal-article","created":{"date-parts":[[2010,3,25]],"date-time":"2010-03-25T03:25:10Z","timestamp":1269487510000},"page":"1683-1689","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":154,"title":["Detection by GenoType MTBDR\n            <i>sl<\/i>\n            Test of Complex Mechanisms of Resistance to Second-Line Drugs and Ethambutol in Multidrug-Resistant\n            <i>Mycobacterium tuberculosis<\/i>\n            Complex Isolates"],"prefix":"10.1128","volume":"48","author":[{"given":"Florence","family":"Brossier","sequence":"first","affiliation":[{"name":"National Reference Centre for Mycobacteria, Ho\u0302pital Pitie\u0301-Salpe\u0302trie\u0300re, AP-HP, Paris, France"},{"name":"Universite\u0301 Pierre et Marie Curie (UPMC), Universite\u0301 Paris 06, Faculte\u0301 de Me\u0301decine Pitie\u0301-Salpe\u0302trie\u0300re, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicolas","family":"Veziris","sequence":"additional","affiliation":[{"name":"National Reference Centre for Mycobacteria, Ho\u0302pital Pitie\u0301-Salpe\u0302trie\u0300re, AP-HP, Paris, France"},{"name":"Universite\u0301 Pierre et Marie Curie (UPMC), Universite\u0301 Paris 06, Faculte\u0301 de Me\u0301decine Pitie\u0301-Salpe\u0302trie\u0300re, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandra","family":"Aubry","sequence":"additional","affiliation":[{"name":"National Reference Centre for Mycobacteria, Ho\u0302pital Pitie\u0301-Salpe\u0302trie\u0300re, AP-HP, Paris, France"},{"name":"Universite\u0301 Pierre et Marie Curie (UPMC), Universite\u0301 Paris 06, Faculte\u0301 de Me\u0301decine Pitie\u0301-Salpe\u0302trie\u0300re, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vincent","family":"Jarlier","sequence":"additional","affiliation":[{"name":"National Reference Centre for Mycobacteria, Ho\u0302pital Pitie\u0301-Salpe\u0302trie\u0300re, AP-HP, Paris, France"},{"name":"Universite\u0301 Pierre et Marie Curie (UPMC), Universite\u0301 Paris 06, Faculte\u0301 de Me\u0301decine Pitie\u0301-Salpe\u0302trie\u0300re, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wladimir","family":"Sougakoff","sequence":"additional","affiliation":[{"name":"National Reference Centre for Mycobacteria, Ho\u0302pital Pitie\u0301-Salpe\u0302trie\u0300re, AP-HP, Paris, France"},{"name":"Universite\u0301 Pierre et Marie Curie (UPMC), Universite\u0301 Paris 06, Faculte\u0301 de Me\u0301decine Pitie\u0301-Salpe\u0302trie\u0300re, Paris, France"},{"name":"Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale (INSERM), Unite\u0301 Mixte de Recherche en Sante\u0301 (UMRS 872, Equipe 12 Pitie\u0301-Salpe\u0302trie\u0300re), Laboratoire de Recherche Mole\u0301culaire sur les Antibiotiques (LRMA), Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.42.5.1295"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.50.1.104-112.2006"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01054-06"},{"key":"e_1_3_2_5_2","first-page":"260","volume":"13","year":"2009","unstructured":"Brossier, F., N. Veziris, V. Jarlier, and W. Sougakoff. 2009. Performance of MTBDRplus for detecting high\/low levels of Mycobacterium tuberculosis resistance to isoniazid. Int. J. Tuber. Lung Dis.13:260-265.","journal-title":"Int. J. Tuber. Lung Dis."},{"key":"e_1_3_2_6_2","first-page":"217","volume":"27","year":"1963","unstructured":"Canetti, G., N. Rist, and J. Grosset. 1963. Measurement of sensitivity of the tuberculous bacillus to antibacillary drugs by the method of proportions. Methodology, resistance criteria, results and interpretation. Rev. Tuberc. Pneumol. (Paris)27:217-272.","journal-title":"Methodology, resistance criteria, results and interpretation. Rev. Tuberc. Pneumol. (Paris)"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.200308-1159OC"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.48.2.596-601.2004"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.3.1311-1315.2003"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00050-09"},{"key":"e_1_3_2_11_2","unstructured":"GenoType MTBDRsl: instruction manual. 2009"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.49.9.3794-3802.2005"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00521-07"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00081-09"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.38.2.238"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2005.02.004"},{"key":"e_1_3_2_17_2","first-page":"634","volume":"128","year":"2008","unstructured":"Jain, A., R. Mondal, S. Srivastava, R. Prasad, K. Singh, and R. C. Ahuja. 2008. Novel mutations in embB gene of ethambutol resistant isolates of Mycobacterium tuberculosis: a preliminary report. Indian J. Med. Res.128:634-639.","journal-title":"Indian J. Med. Res."},{"key":"e_1_3_2_18_2","first-page":"68","volume":"10","year":"2006","unstructured":"Johnson, R., A. M. Jordaan, L. Pretorius, E. Engelke, G. van der Spuy, C. Kewley, M. Bosman, P. D. van Helden, R. Warren, and T. C. Victor. 2006. Ethambutol resistance testing by mutation detection. Int. J. Tuber. Lung Dis.10:68-73.","journal-title":"Int. J. Tuber. Lung Dis."},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.00155708"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.40.8.1768"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.47.9.2971-2973.2003"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00944-06"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.49.8.3192-3197.2005"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.49.2.571-577.2005"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.10.3810-3813.2002"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.50.5.1900-1902.2006"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01486-07"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.40.4.1024"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.38.4.773"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm0597-567"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.2.636-641.2001"},{"key":"e_1_3_2_32_2","unstructured":"Guidelines for drug susceptibility testing for second-line anti-tuberculosis drugs for DOTS-Plus. 2001"},{"key":"e_1_3_2_33_2","doi-asserted-by":"crossref","unstructured":"Anti-tuberculosis drug resistance in the world. 2008","DOI":"10.1016\/S8756-3452(08)70748-5"},{"key":"e_1_3_2_34_2","unstructured":"Guidelines for the programmatic management of drug-resistant tuberculosis: emergency update 2008. 2008"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1086\/315708"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.01947-09","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.01947-09","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T08:45:10Z","timestamp":1646469910000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.01947-09"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,5]]},"references-count":34,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2010,5]]}},"alternative-id":["10.1128\/JCM.01947-09"],"URL":"https:\/\/doi.org\/10.1128\/jcm.01947-09","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,5]]},"assertion":[{"value":"2009-10-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2010-03-16","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2010-05-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}