{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T16:33:05Z","timestamp":1791909185484,"version":"4.3.4"},"reference-count":20,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2007,3,1]],"date-time":"2007-03-01T00:00:00Z","timestamp":1172707200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2007,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            We evaluated a new real-time PCR-based prototype assay for the detection of\n            <jats:italic>Chlamydia trachomatis<\/jats:italic>\n            and\n            <jats:italic>Neisseria gonorrhoeae<\/jats:italic>\n            developed by Abbott Molecular Inc. This assay is designed to be performed on an Abbott\n            <jats:italic>m<\/jats:italic>\n            2000 real-time instrument system, which consists of an\n            <jats:italic>m<\/jats:italic>\n            2000\n            <jats:italic>sp<\/jats:italic>\n            instrument for sample preparation and an\n            <jats:italic>m<\/jats:italic>\n            2000\n            <jats:italic>rt<\/jats:italic>\n            instrument for real-time PCR amplification and detection. The limit of detection of this prototype assay was determined to be 20 copies of target DNA for both\n            <jats:italic>C. trachomatis<\/jats:italic>\n            and\n            <jats:italic>N. gonorrhoeae<\/jats:italic>\n            , using serially diluted linearized plasmids. No cross-reactivity could be detected when 55 nongonococcal\n            <jats:italic>Neisseria<\/jats:italic>\n            isolates and 3 non-\n            <jats:italic>C. trachomatis Chlamydia<\/jats:italic>\n            isolates were tested at 1 million genome equivalents per reaction. Concordance with the Roche Amplicor, BDProbeTec ET, and Gen-Probe APTIMA Combo 2 tests was assessed using unlinked\/deidentified surplus clinical specimens previously analyzed with these tests. For\n            <jats:italic>C. trachomatis<\/jats:italic>\n            , concordance for positive results ranged from 93.7% to 100%, while concordance for negative results ranged from 98.2% to 100%. For\n            <jats:italic>N. gonorrhoeae<\/jats:italic>\n            , concordance for positive and negative results ranged from 91.4% to 100% and 99.3% to 100%, respectively. A workflow analysis of the prototype assay was conducted to obtain information on throughput under laboratory conditions. At 48 samples\/run, the time to first result for both\n            <jats:italic>C. trachomatis<\/jats:italic>\n            and\n            <jats:italic>N. gonorrhoeae<\/jats:italic>\n            was 4.5 h. A total of 135 patient specimens could be analyzed in 8.9 h, with 75 min of hands-on time. This study demonstrated the technical and clinical feasibility of the new Abbott real-time PCR\n            <jats:italic>C. trachomatis<\/jats:italic>\n            \/\n            <jats:italic>N. gonorrhoeae<\/jats:italic>\n            assay.\n          <\/jats:p>","DOI":"10.1128\/jcm.01956-06","type":"journal-article","created":{"date-parts":[[2007,1,4]],"date-time":"2007-01-04T06:30:41Z","timestamp":1167892241000},"page":"747-751","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":47,"title":["Characteristics of the\n            <i>m<\/i>\n            2000 Automated Sample Preparation and Multiplex Real-Time PCR System for Detection of\n            <i>Chlamydia trachomatis<\/i>\n            and\n            <i>Neisseria gonorrhoeae<\/i>"],"prefix":"10.1128","volume":"45","author":[{"given":"R.","family":"Marshall","sequence":"first","affiliation":[{"name":"Abbott Molecular Inc., Des Plaines, Illinois"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Chernesky","sequence":"additional","affiliation":[{"name":"McMaster University\/St. Joseph's Healthcare, Hamilton, Ontario, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Jang","sequence":"additional","affiliation":[{"name":"McMaster University\/St. Joseph's Healthcare, Hamilton, Ontario, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E. 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P.","family":"Cartwright","sequence":"additional","affiliation":[{"name":"University of Minnesota Medical School and Hennepin County Medical Center, Minneapolis, Minnesota"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.","family":"Howell-Adams","sequence":"additional","affiliation":[{"name":"Abbott Molecular Inc., Des Plaines, Illinois"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Ho","sequence":"additional","affiliation":[{"name":"Abbott Molecular Inc., Des Plaines, Illinois"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Welk","sequence":"additional","affiliation":[{"name":"Abbott Molecular Inc., Des Plaines, Illinois"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Lai-Zhang","sequence":"additional","affiliation":[{"name":"Abbott Molecular Inc., Des Plaines, Illinois"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Brashear","sequence":"additional","affiliation":[{"name":"Abbott Molecular Inc., Des Plaines, Illinois"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.","family":"Diedrich","sequence":"additional","affiliation":[{"name":"Abbott Molecular Inc., Des Plaines, Illinois"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Otis","sequence":"additional","affiliation":[{"name":"Abbott Molecular Inc., Des Plaines, Illinois"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.","family":"Webb","sequence":"additional","affiliation":[{"name":"Abbott Molecular Inc., Des Plaines, Illinois"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Robinson","sequence":"additional","affiliation":[{"name":"Abbott Molecular Inc., Des Plaines, 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