{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T02:11:02Z","timestamp":1790475062927,"version":"4.1.0"},"reference-count":23,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2012,12,1]],"date-time":"2012-12-01T00:00:00Z","timestamp":1354320000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2012,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Toxoplasmosis is a worldwide infection that may cause severe disease and is regarded as a serious health problem in France. Detection of the parasite by molecular methods is crucial for diagnosing the disease. The extreme diversity of methods and performances of\n            <jats:named-content content-type=\"genus-species\">Toxoplasma<\/jats:named-content>\n            PCR assays makes the use of commercial PCR kits an attractive alternative, as they offer a chance for standardization. We compared the performances of three molecular methods for the detection of\n            <jats:named-content content-type=\"genus-species\">Toxoplasma gondii<\/jats:named-content>\n            DNA in amniotic fluid: a commercial method using nested PCR and two laboratory-developed methods, one using conventional PCR and the other one real-time PCR. This evaluation was based upon a\n            <jats:named-content content-type=\"genus-species\">T. gondii<\/jats:named-content>\n            DNA serial dilution assay, three amniotic fluid samples spiked with\n            <jats:named-content content-type=\"genus-species\">T. gondii<\/jats:named-content>\n            at different concentrations, and a clinical cohort of 33 amniotic fluid samples. The\n            <jats:named-content content-type=\"genus-species\">T. gondii<\/jats:named-content>\n            DNA serial dilution assay showed a much lower sensitivity for the commercial kit than for the laboratory-developed methods. Moreover, out of 12 proven congenital toxoplasmosis cases, 91.7% were detected by the laboratory-developed assays, whereas only 50% were detected by the commercial kit. A lack of sensitivity of the method, partly due to the presence of PCR inhibitors, was the main drawback of the commercial method. This study emphasizes that commercial PCR diagnostic kits do not systematically perform better than carefully optimized laboratory-developed methods. There is a need for thorough evaluation of such kits by proficient groups, as well as for performance standards that commercial kits can be tested against to improve confidence in those selected by health care providers.\n          <\/jats:p>","DOI":"10.1128\/jcm.01959-12","type":"journal-article","created":{"date-parts":[[2012,10,4]],"date-time":"2012-10-04T05:36:11Z","timestamp":1349328971000},"page":"3977-3982","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":31,"title":["Comparative Assessment of a Commercial Kit and Two Laboratory-Developed PCR Assays for Molecular Diagnosis of Congenital Toxoplasmosis"],"prefix":"10.1128","volume":"50","author":[{"given":"Christelle","family":"Morelle","sequence":"first","affiliation":[{"name":"University Hospital Centre of Montpellier and University Montpellier I, Department of Parasitology-Mycology, Faculty of Medicine, Montpellier, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emmanuelle","family":"Varlet-Marie","sequence":"additional","affiliation":[{"name":"University Hospital Centre of Montpellier and University Montpellier I, Department of Parasitology-Mycology, Faculty of Medicine, Montpellier, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie-Pierre","family":"Brenier-Pinchart","sequence":"additional","affiliation":[{"name":"Parasitology-Mycology, University Hospital A. Michallon and University Joseph Fourier Grenoble I, Grenoble, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sophie","family":"Cassaing","sequence":"additional","affiliation":[{"name":"Department of Parasitology-Mycology, University Hospital Center of Toulouse, Toulouse, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Herv\u00e9","family":"Pelloux","sequence":"additional","affiliation":[{"name":"Parasitology-Mycology, University Hospital A. Michallon and University Joseph Fourier Grenoble I, Grenoble, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick","family":"Bastien","sequence":"additional","affiliation":[{"name":"University Hospital Centre of Montpellier and University Montpellier I, Department of Parasitology-Mycology, Faculty of Medicine, Montpellier, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yvon","family":"Sterkers","sequence":"additional","affiliation":[{"name":"University Hospital Centre of Montpellier and University Montpellier I, Department of Parasitology-Mycology, Faculty of Medicine, Montpellier, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0035-9203(02)90078-7"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2006.01642.x"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02258-07"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1590\/S0074-02762009000200038"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1645\/GE-2405.1"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1051\/parasite\/2007142149"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.44.3.720-724.2006"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.4.1719-1722.2004"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.8.2929-2932.2000"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2005.01332.x"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.45566-0"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199409153311102"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0020-7519(99)00170-8"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cca.2006.06.016"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01701522"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2009.02809.x"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2334-3-7"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2003.09.039"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.2999980"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02500-09"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2010.03101.x"},{"key":"e_1_3_2_23_2","first-page":"43","article-title":"Screening programme for congenital toxoplasmosis in France","volume":"84","author":"Thulliez P","year":"1992","unstructured":"ThulliezP. 1992. 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