{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T00:01:27Z","timestamp":1783036887986,"version":"3.54.6"},"reference-count":34,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2018,6,1]],"date-time":"2018-06-01T00:00:00Z","timestamp":1527811200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2018,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Histoplasmosis is an important cause of mortality in patients with AIDS, especially in countries with limited access to antiretroviral therapies and diagnostic tests. However, many disseminated infections in Latin America go undiagnosed. A simple, rapid method to detect\n            <jats:named-content content-type=\"genus-species\">Histoplasma capsulatum<\/jats:named-content>\n            infection in regions where histoplasmosis is endemic would dramatically decrease the time to diagnosis and treatment, reducing morbidity and mortality. The aim of this study was to validate a commercial monoclonal\n            <jats:named-content content-type=\"genus-species\">Histoplasma<\/jats:named-content>\n            galactomannan (HGM) enzyme-linked immunosorbent assay (Immuno-Mycologics [IMMY], Norman, OK, USA) in two cohorts of people living with HIV\/AIDS (PLHIV). We analyzed urine samples from 589 people (466 from Guatemala and 123 from Colombia), including 546 from PLHIV and 43 from non-PLHIV controls. Sixty-three of these people (35 from Guatemala and 28 from Colombia) had confirmed histoplasmosis by isolation of\n            <jats:named-content content-type=\"genus-species\">H. capsulatum<\/jats:named-content>\n            . Using the standard curve provided by the quantitative commercial test, the sensitivity was 98% (95% confidence interval [CI], 95 to 100%) and the specificity was 97% (95% CI, 96 to 99%) (cutoff = 0.5 ng\/ml). Semiquantitative results, using a calibrator of 12.5 ng\/ml of\n            <jats:named-content content-type=\"genus-species\">Histoplasma<\/jats:named-content>\n            galactomannan to calculate an enzyme immunoassay index value (EIV) for the samples, showed a sensitivity of 95% (95% CI, 89 to 100%) and a specificity of 98% (95% CI, 96 to 99%) (cutoff \u2265 2.6 EIV). This relatively simple-to-perform commercial antigenuria test showed a high performance with reproducible results in both countries, suggesting that it can be used to detect progressive disseminated histoplasmosis in PLHIV in a wide range of clinical laboratories in countries where histoplasmosis is endemic.\n          <\/jats:p>","DOI":"10.1128\/jcm.01959-17","type":"journal-article","created":{"date-parts":[[2018,3,22]],"date-time":"2018-03-22T10:04:18Z","timestamp":1521713058000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":74,"title":["Multicenter Validation of Commercial Antigenuria Reagents To Diagnose Progressive Disseminated Histoplasmosis in People Living with HIV\/AIDS in Two Latin American Countries"],"prefix":"10.1128","volume":"56","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8749-9809","authenticated-orcid":false,"given":"Diego H.","family":"C\u00e1ceres","sequence":"first","affiliation":[{"name":"Medical and Experimental Mycology Group, Corporaci\u00f3n para Investigaciones Biol\u00f3gicas (CIB), Medell\u00edn, Colombia"},{"name":"Mycotic Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Blanca E.","family":"Samayoa","sequence":"additional","affiliation":[{"name":"Asociaci\u00f3n de Salud Integral, Guatemala City, Guatemala"},{"name":"Facultad de Ciencias Qu\u00edmicas y Farmacia, Universidad de San Carlos de Guatemala, Guatemala City, Guatemala"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Narda G.","family":"Medina","sequence":"additional","affiliation":[{"name":"Asociaci\u00f3n de Salud Integral, Guatemala City, Guatemala"},{"name":"Cl\u00ednica Familiar Luis \u00c1ngel Garc\u00eda, Hospital General San Juan de Dios, Guatemala City, Guatemala"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angela M.","family":"Tob\u00f3n","sequence":"additional","affiliation":[{"name":"Medical and Experimental Mycology Group, Corporaci\u00f3n para Investigaciones Biol\u00f3gicas (CIB), Medell\u00edn, Colombia"},{"name":"Hospital La Mar\u00eda, Medell\u00edn, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brenda J.","family":"Guzm\u00e1n","sequence":"additional","affiliation":[{"name":"Cl\u00ednica Familiar Luis \u00c1ngel Garc\u00eda, Hospital General San Juan de Dios, Guatemala City, Guatemala"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danicela","family":"Mercado","sequence":"additional","affiliation":[{"name":"Asociaci\u00f3n de Salud Integral, Guatemala City, Guatemala"},{"name":"Cl\u00ednica Familiar Luis \u00c1ngel Garc\u00eda, Hospital General San Juan de Dios, Guatemala City, Guatemala"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angela","family":"Restrepo","sequence":"additional","affiliation":[{"name":"Medical and Experimental Mycology Group, Corporaci\u00f3n para Investigaciones Biol\u00f3gicas (CIB), Medell\u00edn, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tom","family":"Chiller","sequence":"additional","affiliation":[{"name":"Mycotic Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eduardo E.","family":"Arathoon","sequence":"additional","affiliation":[{"name":"Asociaci\u00f3n de Salud Integral, Guatemala City, Guatemala"},{"name":"Cl\u00ednica Familiar Luis \u00c1ngel Garc\u00eda, Hospital General San Juan de Dios, Guatemala City, Guatemala"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6641-1924","authenticated-orcid":false,"given":"Beatriz L.","family":"G\u00f3mez","sequence":"additional","affiliation":[{"name":"Medical and Experimental Mycology Group, Corporaci\u00f3n para Investigaciones Biol\u00f3gicas (CIB), Medell\u00edn, Colombia"},{"name":"School of Medicine and Health Sciences, Universidad del Rosario, Bogota, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/s40475-015-0044-0"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.3109\/13693786.2011.577821"},{"key":"e_1_3_2_4_2","volume-title":"Enfermedades infecciosas de Homo sapiens","author":"Tobon A","year":"2015","unstructured":"Tobon A, Gomez BL. 2015. Histoplasmosis. In Vesga O, V\u00e9lez LA, Leiderman E, Restrepo A (ed), Enfermedades infecciosas de Homo sapiens, 1st ed. Fondo Editorial CIB, Medellin, Colombia.","edition":"1"},{"key":"e_1_3_2_5_2","first-page":"2949","volume-title":"Principles and practice of infectious diseases","author":"Deepe GS","year":"2015","unstructured":"Deepe GS. 2015. Histoplasma capsulatum, p 2949\u20132962. In Bennett JE, Dolin R, Blaser MJ (ed). Principles and practice of infectious diseases, 8th ed. 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