{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,24]],"date-time":"2026-03-24T16:16:19Z","timestamp":1774368979312,"version":"3.50.1"},"reference-count":65,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2020,12,17]],"date-time":"2020-12-17T00:00:00Z","timestamp":1608163200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000016","name":"U.S. Department of Health and Human Services","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000016","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2020,12,17]]},"abstract":"<jats:p>\n            There are over 40 species within the genus\n            <jats:italic>Entamoeba<\/jats:italic>\n            , eight of which infect humans. Of these, four species (\n            <jats:named-content content-type=\"genus-species\">Entamoeba histolytica<\/jats:named-content>\n            ,\n            <jats:italic>E. dispar<\/jats:italic>\n            ,\n            <jats:italic>E. moshkovskii<\/jats:italic>\n            , and\n            <jats:italic>E. bangladeshi<\/jats:italic>\n            ) are morphologically indistinguishable from each other, and yet differentiation is important for appropriate treatment decisions. Here, we developed a hydrolysis probe-based tetraplex real-time PCR assay that can simultaneously detect and differentiate these four species in clinical samples.\n          <\/jats:p>","DOI":"10.1128\/jcm.01986-20","type":"journal-article","created":{"date-parts":[[2020,10,27]],"date-time":"2020-10-27T14:34:57Z","timestamp":1603809297000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":10,"title":["A Real-Time PCR Assay for Simultaneous Detection and Differentiation of Four Common\n            <i>Entamoeba<\/i>\n            Species That Infect Humans"],"prefix":"10.1128","volume":"59","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9808-3227","authenticated-orcid":false,"given":"Ibne Karim M.","family":"Ali","sequence":"first","affiliation":[{"name":"Free-Living and Intestinal Amebas (FLIA) Laboratory, Waterborne Disease Prevention Branch, Division of Foodborne, Waterborne, and Environmental Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shantanu","family":"Roy","sequence":"additional","affiliation":[{"name":"Free-Living and Intestinal Amebas (FLIA) Laboratory, Waterborne Disease Prevention Branch, Division of Foodborne, Waterborne, and Environmental Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(12)61728-0"},{"key":"e_1_3_2_3_2","first-page":"97","article-title":"World Health Organization\/Pan American Health Organization\/UNESCO report of a consultation of experts on amebiasis","volume":"72","author":"Anonymous","year":"1997","unstructured":"Anonymous. 1997. 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