{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:29:44Z","timestamp":1787016584676,"version":"build-2736575974"},"reference-count":52,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2017,3,1]],"date-time":"2017-03-01T00:00:00Z","timestamp":1488326400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"Research Fund of Hospital District of Southwest Finland"},{"DOI":"10.13039\/100007797","name":"University of Helsinki","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100007797","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100005430","name":"American Society for Microbiology","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100005430","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2017,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Reliable distinction of\n                    <jats:named-content content-type=\"genus-species\">Streptococcus pneumoniae<\/jats:named-content>\n                    and viridans group streptococci is important because of the different pathogenic properties of these organisms. Differentiation between\n                    <jats:named-content content-type=\"genus-species\">S. pneumoniae<\/jats:named-content>\n                    and closely related\n                    <jats:named-content content-type=\"genus-species\">Sreptococcus<\/jats:named-content>\n                    <jats:italic>mitis<\/jats:italic>\n                    species group streptococci has always been challenging, even when using such modern methods as 16S rRNA gene sequencing or matrix-assisted laser desorption ionization\u2013time of flight (MALDI-TOF) mass spectrometry. In this study, a novel algorithm combined with an enhanced database was evaluated for differentiation between\n                    <jats:named-content content-type=\"genus-species\">S. pneumoniae<\/jats:named-content>\n                    and\n                    <jats:named-content content-type=\"genus-species\">S. mitis<\/jats:named-content>\n                    species group streptococci. One hundred one clinical\n                    <jats:named-content content-type=\"genus-species\">S. mitis<\/jats:named-content>\n                    species group streptococcal strains and 188 clinical\n                    <jats:named-content content-type=\"genus-species\">S. pneumoniae<\/jats:named-content>\n                    strains were identified by both the standard MALDI Biotyper database alone and that combined with a novel algorithm. The database update from 4,613 strains to 5,627 strains drastically improved the differentiation of\n                    <jats:named-content content-type=\"genus-species\">S. pneumoniae<\/jats:named-content>\n                    and\n                    <jats:named-content content-type=\"genus-species\">S. mitis<\/jats:named-content>\n                    species group streptococci: when the new database version containing 5,627 strains was used, only one of the 101\n                    <jats:named-content content-type=\"genus-species\">S. mitis<\/jats:named-content>\n                    species group isolates was misidentified as\n                    <jats:named-content content-type=\"genus-species\">S. pneumoniae<\/jats:named-content>\n                    , whereas 66 of them were misidentified as\n                    <jats:named-content content-type=\"genus-species\">S. pneumoniae<\/jats:named-content>\n                    when the earlier 4,613-strain MALDI Biotyper database version was used. The updated MALDI Biotyper database combined with the novel algorithm showed even better performance, producing no misidentifications of the\n                    <jats:named-content content-type=\"genus-species\">S. mitis<\/jats:named-content>\n                    species group strains as\n                    <jats:named-content content-type=\"genus-species\">S. pneumoniae<\/jats:named-content>\n                    . All\n                    <jats:named-content content-type=\"genus-species\">S. pneumoniae<\/jats:named-content>\n                    strains were correctly identified as\n                    <jats:named-content content-type=\"genus-species\">S. pneumoniae<\/jats:named-content>\n                    with both the standard MALDI Biotyper database and the standard MALDI Biotyper database combined with the novel algorithm. This new algorithm thus enables reliable differentiation between pneumococci and other\n                    <jats:named-content content-type=\"genus-species\">S. mitis<\/jats:named-content>\n                    species group streptococci with the MALDI Biotyper.\n                  <\/jats:p>","DOI":"10.1128\/jcm.01990-16","type":"journal-article","created":{"date-parts":[[2017,1,4]],"date-time":"2017-01-04T21:40:28Z","timestamp":1483566028000},"page":"914-922","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":45,"title":["Improved Differentiation of Streptococcus pneumoniae and Other S. mitis Group Streptococci by MALDI Biotyper Using an Improved MALDI Biotyper Database Content and a Novel Result Interpretation Algorithm"],"prefix":"10.1128","volume":"55","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2767-9127","authenticated-orcid":false,"given":"Inka","family":"Harju","sequence":"first","affiliation":[{"name":"Clinical Microbiology Laboratory, Turku University Hospital, Turku, Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christoph","family":"Lange","sequence":"additional","affiliation":[{"name":"Bruker Daltonik GmbH, Bremen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Markus","family":"Kostrzewa","sequence":"additional","affiliation":[{"name":"Bruker Daltonik GmbH, Bremen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Maier","sequence":"additional","affiliation":[{"name":"Bruker Daltonik GmbH, Bremen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaisu","family":"Rantakokko-Jalava","sequence":"additional","affiliation":[{"name":"Clinical Microbiology Laboratory, Turku University Hospital, Turku, Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marjo","family":"Haanper\u00e4","sequence":"additional","affiliation":[{"name":"Bacterial Infections Unit, National Institute for Health and Welfare, Helsinki, Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1128\/9781555816728.ch20","volume-title":"Manual of clinical microbiology","author":"Spellerberg B","year":"2011","unstructured":"Spellerberg B Brandt C . 2011. 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