{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,18]],"date-time":"2026-05-18T14:34:08Z","timestamp":1779114848327,"version":"3.51.4"},"reference-count":30,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2021,1,21]],"date-time":"2021-01-21T00:00:00Z","timestamp":1611187200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"Sante Publique France"},{"DOI":"10.13039\/501100005897","name":"Soci\u00e9t\u00e9 Fran\u00e7aise de Dermatologie et de Pathologie Sexuellement Transmissible","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005897","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006688","name":"Association de Recherche en Virologie et Dermatologie","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006688","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2021,1,21]]},"abstract":"<jats:p>\n            We evaluated the utility of the commercial Allplex genital ulcer real-time PCR multiplex assay for detecting\n            <jats:named-content content-type=\"genus-species\">Treponema pallidum<\/jats:named-content>\n            (TPA), herpes simplex virus 1 (HSV-1) and 2 (HSV-2), and\n            <jats:named-content content-type=\"genus-species\">Chlamydia trachomatis<\/jats:named-content>\n            serovar L (lymphogranuloma venereum [LGV]) DNA in mucosal and genital ulcers in the context of suspected syphilis. In total, 374 documented genital and mucosal ulcers from patients with and without syphilis presenting at several sexually transmitted infection (STI) centers in France from October 2010 to December 2016 were analyzed at the National Reference Center (CNR) for Bacterial STIs at Cochin Hospital in Paris. TPA detection results were compared with the final diagnosis based on a combination of clinical examination, serological results, and in-house nested PCR (nPCR).\n          <\/jats:p>","DOI":"10.1128\/jcm.01994-20","type":"journal-article","created":{"date-parts":[[2020,11,10]],"date-time":"2020-11-10T14:16:25Z","timestamp":1605017785000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":21,"title":["Use of a Multiplex PCR Assay To Assess the Presence of Treponema pallidum in Mucocutaneous Ulcerations in Patients with Suspected Syphilis"],"prefix":"10.1128","volume":"59","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1583-0456","authenticated-orcid":false,"given":"P. 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