{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T14:09:14Z","timestamp":1792591754989,"version":"4.5.0"},"reference-count":31,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2018,5,1]],"date-time":"2018-05-01T00:00:00Z","timestamp":1525132800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2018,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    One reason for increased pertussis incidence is the adaptation of\n                    <jats:named-content content-type=\"genus-species\">Bordetella pertussis<\/jats:named-content>\n                    to vaccine-induced immunity by modulating its genomic structure. This study, EUpert IV, includes 265 isolates collected from nine European countries during 2012 to 2015 (\n                    <jats:italic>n<\/jats:italic>\n                    = 265) and compares the results to previous EUpert I to III studies (1998 to 2009). The analyses included genotyping, serotyping, pulsed-field gel electrophoresis (PFGE), and multilocus variable-number tandem-repeat analysis (MLVA). Genotyping results showed only small variations among the common virulence genes of\n                    <jats:named-content content-type=\"genus-species\">B. pertussis<\/jats:named-content>\n                    . The frequencies of serotypes Fim2 and Fim3 varied among the four collections. Genomic analyses showed that MLVA type 27 increased to 80% between the periods of 1998 to 2001 and 2012 to 2015. Two PFGE profiles, BpSR3 (29.4%) and BpSR10 (27.2%), constituted more than 50% of the circulating isolates in the present collection. Our study indicates that the European\n                    <jats:named-content content-type=\"genus-species\">B. pertussis<\/jats:named-content>\n                    population is changing and became more homogenous after the introduction of acellular pertussis vaccines.\n                  <\/jats:p>","DOI":"10.1128\/jcm.01998-17","type":"journal-article","created":{"date-parts":[[2018,3,1]],"date-time":"2018-03-01T08:02:00Z","timestamp":1519891320000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":39,"title":["Surveillance of Circulating Bordetella pertussis Strains in Europe during 1998 to 2015"],"prefix":"10.1128","volume":"56","author":[{"given":"Alex-Mikael","family":"Barkoff","sequence":"first","affiliation":[{"name":"Department of Medical Microbiology and Immunology, University of Turku, Turku, 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