{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T01:34:08Z","timestamp":1777426448230,"version":"3.51.4"},"reference-count":32,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2013,1,1]],"date-time":"2013-01-01T00:00:00Z","timestamp":1356998400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2013,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Although malaria remains one of the leading infectious diseases in the world, the decline in malaria transmission in some area makes it possible to consider elimination of the disease. As countries approach elimination, malaria diagnosis needs to change from diagnosing ill patients to actively detecting infections in all carriers, including asymptomatic and low-parasite-load patients. However, few of the current diagnostic methods have both the throughput and the sensitivity required. We adopted a sandwich RNA hybridization assay to detect genus\n            <jats:named-content content-type=\"genus-species\">Plasmodium<\/jats:named-content>\n            18S rRNA directly from whole-blood samples from\n            <jats:named-content content-type=\"genus-species\">Plasmodium falciparum<\/jats:named-content>\n            and\n            <jats:named-content content-type=\"genus-species\">Plasmodium vivax<\/jats:named-content>\n            patients without RNA isolation. We tested the assay with 202 febrile patients from areas where malaria is endemic, using 20 \u03bcl of each blood sample in a 96-well plate format with a 2-day enzyme-linked immunosorbent assay (ELISA)-like work flow. The results were compared with diagnoses obtained using microscopy, a rapid diagnostic test (RDT), and genus-specific real-time PCR. Our assay identified all 66 positive samples diagnosed by microscopy, including 49 poorly stored samples that underwent multiple freeze-thaw cycles due to resource limitation. The assay uncovered three false-negative samples by microscopy and four false-negative samples by RDT and agreed completely with real-time PCR diagnosis. There was no negative sample by our assay that would show a positive result when tested with other methods. The detection limit of our assay for\n            <jats:named-content content-type=\"genus-species\">P. falciparum<\/jats:named-content>\n            was 0.04 parasite\/\u03bcl. The assay's simple work flow, high throughput, and sensitivity make it suitable for active malaria screening.\n          <\/jats:p>","DOI":"10.1128\/jcm.02010-12","type":"journal-article","created":{"date-parts":[[2012,10,25]],"date-time":"2012-10-25T04:29:53Z","timestamp":1351139393000},"page":"125-130","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":17,"title":["A Novel, Sensitive Assay for High-Throughput Molecular Detection of Plasmodia for Active Screening of Malaria for Elimination"],"prefix":"10.1128","volume":"51","author":[{"given":"Zhibin","family":"Cheng","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and School of Basic Medicine, Peking Union Medical College, Beijing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaodong","family":"Sun","sequence":"additional","affiliation":[{"name":"Yunnan Institute of Parasitic Diseases, Puer, Yunnan, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ye","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Parasitology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and School of Basic Medicine, Peking Union Medical College, Beijing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heng","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Parasitology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and School of Basic Medicine, Peking Union Medical College, Beijing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhi","family":"Zheng","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and School of Basic Medicine, Peking Union Medical College, Beijing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.1000406"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(10)61500-0"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.0050038"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1186\/1475-2875-9-254"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1186\/1475-2875-9-30"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1186\/1475-2875-7-17"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1086\/599317"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1603\/0022-2585-41.2.201"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1996.54.325"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2007.76.470"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.15.1.66-78.2002"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1086\/644781"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/0166-6851(93)90050-8"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.12.5636-5643.2004"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00276-11"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1186\/1475-2875-5-80"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0013733"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.1000396"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(09)70247-6"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.39073.496829.AE"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.c930"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.39395.696065.47"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.1001142"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.1000400"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2005.065078"},{"key":"e_1_3_2_27_2","volume-title":"Assessment of therapeutic efficacy of antimalarial drugs for uncomplicated falciparum malaria in areas with intense transmission","author":"World Health Organization","year":"1996","unstructured":"World Health Organization. 1996. Assessment of therapeutic efficacy of antimalarial drugs for uncomplicated falciparum malaria in areas with intense transmission. World Health Organization, Geneva, Switzerland."},{"key":"e_1_3_2_28_2","unstructured":"World Health Organization.\n2010.\nInformation note on recommended selection criteria for malaria rapid diagnostic tests (RDTs).\nhttp:\/\/www.who.int\/malaria\/diagnosis_treatment\/diagnosis\/RDT_selection_criteria.pdf.\nAccessed 9 October 2011."},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/15.5.348"},{"key":"e_1_3_2_30_2","first-page":"78","volume-title":"Bruce-Chwatts essential malariology","author":"Gilles HM","year":"1993","unstructured":"GillesHM . 1993. Diagnostic methods in malaria, p 78\u201399. In GillesHM WarrellDA (ed), Bruce-Chwatts essential malariology, 3rd ed. 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Accessed 9 October 2011."}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.02010-12","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.02010-12","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T11:29:44Z","timestamp":1646479784000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.02010-12"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,1]]},"references-count":32,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2013,1]]}},"alternative-id":["10.1128\/JCM.02010-12"],"URL":"https:\/\/doi.org\/10.1128\/jcm.02010-12","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,1]]},"assertion":[{"value":"2012-08-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2012-10-15","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-12-26","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}